Yue Cong

Alkaloid profiling of crude and processed Veratrum nigrum L. through simultaneous determination of ten steroidal alkaloids by HPLC–ELSD

Veratrum nigrum L., a traditional Chinese herb, has been used for treatment of hypertension, blood-stroke, excessive phlegm, epilepsy, etc. Steroidal alkaloids were well-known as both bioactive and toxic constituents of Veratrum species, the toxicity of which the traditional processing procedure can reduce. To reveal the mechanism of processing V. nigrum L., a high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) method was developed for the simultaneous determination of ten steroidal alkaloids in crude and processed V. nigrum L., comparison with ultrasound extract of crude V. nigrum L. With a Venusil XBP-C(18) analytical column, the analytes were separated efficiently using the mobile phase consisted of (A) 0.03% aqueous triethylamine (TEA) and (B) acetonitrile in a gradient program. The parameters for ELSD were set: S.C. (Spray Chamber)=35 degrees C, D.T. (Drift Tube)=70 degrees C, GAS=50 psi. All calibration curves showed good linear regression (gamma>or=0.9990) within the tested range. Additionally, reproducibility for the quantification of ten alkaloids in V. nigrum L. with intra- and inter-day variations of less than 5.0% was observed. The obtained alkaloid profiles performed by this newly established method, provided valuable information for the differentiation of crude and processed V. nigrum L. and for the explanation of the different toxicity.

A new flavonoside from Leonurus heterophyllus

In a chemical investigation on the flavone composition of Leonurus heterophyllus a new flavonoside has been isolated. By means of physico-chemical evidences and spectral analysis its structure has been established as quercetin-3-O-[3-(4-hydroxy-3,5-dimethoxybenzyl)-α-l-rhamnopyranosyl]-(1→6)-β-d-galactopyranoside (1).

Chemical constituents of radix Ranunculus ternati.

3β-Acetoxy-(20S, 22E)-dammaran-22-en-25-ol, a new triterpene, was isolated along with five known triterpenes (ursolic acid, oleanolic acid, betulinic acid, 3-epiocotillol acetate, and dimmarenediol II acetate), and α-D-glc and sucrose from Radix Ranunculus ternati All of them, except oleanolic acid and α-D-glc, were isolated from the family of Ranunculaceae for the very first time, and the NMR data of sucrose was first described. In addition, the absolute configurations of α-D-glc and the glucose component of sucrose were determined.

A study on the chemical constituents of Veratrum nigrum L. processed by rice vinegar.

One new steroid alkaloid, 12β-hydroxylveratroylzygadenine (1) and four known compounds, verdine (2), jervine (3), veramarine (4), and veratroylzygadenine (5), have been isolated from the roots and rhizomes of Veratrum nigrum L. processed by rice vinegar. Their structures were established through a combined analysis of physicochemical properties and spectroscopic evidence. The assay results revealed that compounds 1, 4, and 5 exhibited cell toxicity against human HL-60 cells with IC50 values 52.67, 52.90, and 56.51 μmol/l, respectively.

A new diterpene from the processed roots of Euphorbia Kansui

A new diterpene has been isolated from the processed roots of Euphorbia Kansui. By means of physico-chemical evidences and spectral analysis, the structure was identified as 4-O-acetyl-5-O-benzoyl-3β-hydroxy-20-deoxyingenol (1).