Yueyuan Chen

Studies on chemical fingerprints of Siraitia grosvenorii fruits (Luo Han Guo) by HPLC

A novel, efficient, and accurate fingerprinting method using high performance liquid chromatography–photodiode array detection has been developed and optimized for the investigation and demonstration of the variance in chemical properties among Siraitia grosvenorii fruits from different origins. The effects of growth stages, cultivated varieties, collection locations, and fruit portions of the herb on chromatographic fingerprints were examined. Eleven compounds were identified on chromatograms by comparing the retention time and UV spectrum of each peak separately with those of external references. The results revealed that chromatographic fingerprints, combining similarity or hierarchical clustering analysis along with reference compounds, could efficiently identify and distinguish S. grosvenorii fruits from different sources, which provided helpful clues for studying the plants’ secondary metabolites and benefitted quality control.

New Pregnane Glycosides from Brucea javanica and Their Antifeedant Activity

Three new pregnane glycosides, 3‐O‐β‐D‐glucopyranosyl‐(1→2)‐α‐L‐arabinopyranosyl‐(20R)‐pregn‐5‐ene‐3β,20‐diol (1), 3‐O‐α‐L‐arabinopyranosyl‐(20R)‐pregn‐5‐ene‐3β,20‐diol‐20‐O‐β‐D‐glucopyranoside (2), 3‐O‐α‐L‐arabinopyranosyl‐(20R)‐pregn‐5‐ene‐3β,20‐diol‐20‐O‐β‐D‐glucopyranosyl‐(1→2)‐β‐D‐glucopyranoside (3) were isolated along with four known compounds, 4–7, from the leaves and stems of Brucea javanica. Their structures were determined by detailed analyses of 1D‐ and 2D‐NMR spectroscopic data. All of the compounds isolated from Brucea javanica were tested for the antifeedant activities against the larva of Pieris rapae. Compounds 1, 3, and 5 showed significant antifeedant activities after 72 h incubation.

Preparative isolation and purification of five phenylethanoid glycosides from Chirita eburnea

0009-3130/11/4704-0615 2011 Springer Science+Business Media, Inc. Department of Phytochemistry, Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and Chinese Academy of Science, Guilin 541006, Guangxi, P. R. China, fax: 86 773 3550067, e-mail: phytoldp@hotmail.com. Published in Khimiya Prirodnykh Soedinenii, No. 4, pp. 545–547, July–August, 2011. Original article submitted March 25, 2010. Chemistry of Natural Compounds, Vol. 47, No. 4, September, 2011 [Russian original No. 4, July–August, 2011]

Phenolic Compounds from the Leaves of Castanopsis fargesii.

In the course of a phytochemical and chemotaxonomical investigation of Castanopsis species (Fagaceae), three new phenolic compounds, (3R,1′S)-[1′-(6″-O-galloyl-β-d-gluco-pyranosyl)oxyethyl]-3-hydroxy-dihydrofuran-2(3H)-one (1), (2R,3S)-2-[2′-(galloyl)oxyethyl]-dihydroxybutanoic acid (2), and (3S,4S)-3-hydroxymethyl-3,4-dihydro-5,6,7-trihydroxy-4-(4′-hydroxy-3′-methoxyphenyl)-1H-[2]-benzopyran-1-one (3) were isolated from the fresh leaves of Castanopsis fargesii. In addition, a known phenolic glycoside, gentisic acid 5-O-α-l-rhamnopyranosyl-(1→2)-β-d-glucopyranoside (4) was also isolated and identified. Their structures were elucidated by means of spectroscopic methods including one- and two-dimensional NMR techniques.