hong Yu

Chromatographic fingerprint analysis of Cephalotaxus sinensis from various sources by high-performance liquid chromatography-diodearray detection-electrospray ionization-tandem mass spectrometry

Selective and efficient analytical methods are required not only for quality assurance but also for authentication of Chinese herbal medicine. A simple, rapid and valid fingerprint method has been first carried out for the quality control of Cephalotaxus sinensis by using high-performance liquid chromatography (HPLC) coupled with photodiode array detection (DAD) and electrospray ionisation-tandem mass spectrometry (ESI-MS). The characteristic analytical fingerprints of this plant extract showed 18 common peaks, and out of these, 10 compounds involving 2 new compounds were identified by comparing the retention time, UV and ESI-MS/MS spectrum of each standard with those of each peak separated by on-line HPLC-DAD-MS/MS. Moreover, the effects of collecting locations, harvesting time, storage time, drying methods, and medicinal portions on herbal chromatographic fingerprints were examined by similarity analysis and principal component analysis (PCA) along with markers. Using the reference fingerprint along with markers, the best harvesting time, cultivation location and medicinal part were determined. The results obtained suggest that the chromatographic fingerprint combining similarity evaluation and PCA along with markers or pharmacologically active constituents can efficiently identify raw herb of Cephalotaxus sinensis from different sources, which provide helpful clues to the study of plants secondary metabolites and benefit quality control.

Appraisal of antinociceptive and anti-inflammatory potential of extract and fractions from the leaves of Torreya grandis Fort Ex. Lindl

Torreya grandis (Taxaceae) was studied for antinociceptive and anti-inflammatory effects. Leaves were extracted with 80% ethanol at 80 degrees C for 3h and fractionated with petroleum ether, chloroform, ethylacetate and n-butanol. The aqueous ethanolic extract (aq.EE), ethylacetate fraction (EaF) and butanol fraction (BtF) at the doses of (100 and 200mg/kg, i.g.) body weight were used for study. Evaluation of antinociceptive activity was carried out by acetic acid-induced writhing response and formalin-induced paw licking time in the first and second phases of mice. The paw edema induced by formalin- and xylene-induced ear edema were used to assess anti-inflammatory activity. It was found that Torreya grandis extract and fractions at the doses of (100 and 200mg/kg, i.g.) were significantly attenuated the writhing responses induced by acetic acid and second phase of pain response induced by subplantar injection of formalin in mice. In addition, these extract and fractions inhibiting the formaldehyde-induced arthritis as well as xylene-induces edema prolifically. From acute oral toxicity studies no mortality was pragmatic even at highest dose (2500 mg/kg, i.g.). Furthermore, our phytochemical studies indicated that the aq. ethanolic extract of leaves contains alkaloids, flavonoids, tannins, terpenoids and saponins. The results provide justification for the folkloric uses of Torreya grandis in the treatment of analgesic and inflammatory-based diseases across the China.

Combination of HPLC chromatogram and hypoglycemic effect identifies isoflavones as the principal active fraction of Belamcanda chinensis leaf extract in diabetes treatment.

In previous study, we demonstrated the hypoglycemic effect of aqueous extract of Belamcanda chinensis leaves in rats. Here, we separated the aqueous extract of B. chinensis leaves and investigated the spectrum-effect relationships between HPLC chromatograms and hypoglycemic activities of different isolates from B. chinensis leaf extract. Sequential solvent extraction with petroleum ether, chloroform, acetic ester and n-butanol provided several isolates showing similar hypoglycemic activities, making it difficult to discriminate the active fractions. Stepwise elution through HP20 macroporous resin by water, 40% and 95% ethanol provided isolates with distinct hypoglycemic activities, representing a simple, rapid and efficient preparative separation method. Combination of HPLC chromatogram and pharmacological effect targeted a hypoglycemic activity-related region in HPLC chromatogram. Each peak in this region was analyzed by UV spectrum scan. Most of them were flavonoids in which tectoridin and swertisin were known flavonoids with anti-diabetic activities. In together, this work provides a general model of combination of HPLC chromatography and pharmacological effect to study the spectrum-effect relationships of aqueous extract from B. chinensis leaves, which can be used to find principle components of B. chinensis on pharmacological activity.

Radical scavenging activity of various fractions of Cephalotaxus sinensis (Rehd et Wile) Li

Cephalotaxus sinensis (Rehd et Wile) Li, belongs to family Cephalotaxaceae, common in China, is an indigenous medicinal plant due to its antiascariasis, antiinflammation, anticough and antidyspepsia activity. The overindulgence of free radicals has been concerned in the development of chronic diseases and play an imperative role in the development of neurodegenerative disorders, such as Alzheimers and Parkinsons diseases. Various fractions of this plant were evaluated for their radical scavenging activity by the DPPHldr and reducing power activity assays. Also, the Folin-Ciocalteu method was used to determine the total phenolic content. The results of both methods revealed that Cephalotaxus. sinensis possesses potent antioxidant activity. The polyphenolic content and antioxidant activities of the different fractions tested decreased in the order of EaF > BtF > CfF > WtF > PtF. The radical scavenging activity of the ethyl acetate fraction higher than BHT and slightly lower than Vit-C. A positive linear correlation between antioxidant activity and contents of polyphenol was established. This information shows that ethyl acetate fraction of C. sinensis can be used as antioxidant in medicinal preparations.

Study on the influence of Konjac glucomannan on assaying domperidone in orally disintergrating tablets

To evaluate the influence of Konjac glucomannan (KGM) on assaying domperidone in orally disintergrating tablets, We established an RP-HPLC method for the determination of domperidone in dompoeridone orally disintergrating tablets. Then KGM was added to the prescription. Good resolution was obtained after the sample was destroyed by acid, alkali and heat. The results suggested that KGM had the potential to be used in drug delivery systems.

Optimization of Condition for Maximum Recovery of Antioxidants from Cephalotaxus sinensis and their FRAP Assay

In this study, Cephalotaxus sinensis (Rehd et Wile) Li (Cephalotaxaeeae), plant was examined for phenolic constituents and antioxidant capacity, to determine their potential as a source of naturat antioxidants. Total polyphenol content (TPC) were determined spectrophotometrically, after extraction of ptant material with assorted solvents at 80degC for 3 hour. Antioxidant activity of aqueous extracts and fractions were evaluated by FRAP assay. The highest antioxidant activity was observed in the extract of 80% aq. ethanol followed by fractionation with 40% (v/v) aq. ethanoi using AB-8 resin, which had soaring leveis of potyphenot. Resutts strongty suggested that potyphenot content shoufd be considered as an imperative feature of Cephalotaxus sinensis ptant and demonstrate a impending for exptoit as natural antioxidants.