Yukihiko Miyayama

OBSERVATIONS OF PRIMORDIAL GERM CELLS IN THE TURTLE EMBRYO (CARETTA CARETTA): LIGHT AND ELECTRON MICROSCOPIC STUDIES

Primordial germ cells (PGCs) in the turtle embryo (Caretta caretta) were observed with light and transmission electron microscopes. Identification of the PGCs for light microscopy was made by the periodic acid‐Schiff (PAS) technique. PGCs were first found in the yolk‐sac endoderm through the 5th to 6th day of development. PGCs freed from the endoderm then migrated to the root area of the dorsal mesentery and the coelomic angle between the 7th and the 11th day of development, and finally settled down in the gonadal anlage by the 14th day. Turtle PGCs were characterized by a large size (16 μm in diameter) and large nuclei with distinct nucleoli, and by the presence of large numbers of lipid droplets, yolk platelets and glycogen particles in the cytoplasm. Cell organelles were well‐developed in PGCs at later stages. Amoeboid features of the PGCs were observed in the mesenchyme, indicating active locomotion. PGCs were usually surrounded or encircled by neighboring somatic cells. No intravascular PGCs were detected at any stage of development examined.