Yuli Yang

Enhanced nitrogen removal by membrane-aerated nitritation-anammox in a bioelectrochemical system

A bioelectrochemical system (BES) containing membrane-aerated nitritation-anammox in its cathode has been developed for enhancing nitrogen removal. Long-term performance and microbial community structure were investigated. The BES using loop operation and external voltage achieved the highest total nitrogen removal efficiency of 94.8±7.7%, and COD removal of 98.2±3.3% at hydraulic retention time of 60h and the lumen pressure of 10psi. The energy consumption of the system was 0.90kWhkgN-1 or 0.38kWhkg COD-1. Sequencing analyses revealed that ammonia oxidizing bacteria (0.2-7.4%), anammox bacteria (0.4-10.3%), denitrifying bacteria (5.8-13.1%), and electrogenic bacteria (4.6-12.8%) were in abundance of the microbial community in the cathode chamber, and their distributions were affected by the aeration and physical locations. These results encourage further investigation of membrane-aerated nitritation-anammox in BES for optimization and potential applications with actual wastewater.

Membrane aeration as an energy-efficient method for supplying oxygen to microbial fuel cells

An active supply of oxygen will help with electricity generation in microbial fuel cells (MFCs). Although air-cathode MFCs have been developed to eliminate aeration, the MFCs with active aeration could have their niches in system scaling up and/or removal of certain contaminants that require oxygen in the cathode. In this study, an alternative aeration method based on a gas-transfer membrane has been investigated for MFC applications, in comparison with diffused aeration. The membrane-aerated microbial fuel cell (MAMFC) achieves a maximum coulombic efficiency (CE) of 55.4%, a current density of 17.3 A m−3 and COD removal efficiency of >61%. The CE of the MAMFC is higher than that of the diffused aeration MFC (DAMFC), indicating a higher conversion efficiency of substrate to electricity with membrane aeration. At the similar dissolved oxygen level of 6.61 mg O2 L−1, the MAMFC requires an energy input of 0.05 kW h m−3, significantly lower than 1.76 kW h m−3 by the DAMFC. Although both MFCs have negative energy balances under the testing conditions, the MAMFC could theoretically save 588–3485% of energy compared with the DAMFC. This study demonstrates that membrane aeration could be an energy efficient method for providing an active oxygen supply for MFC applications.

A continuous flow MFC-CW coupled with a biofilm electrode reactor to simultaneously attenuate sulfamethoxazole and its corresponding resistance genes

A continuous flow microbial fuel cell constructed wetland (MFC-CW) coupled with a biofilm electrode reactor (BER) system was constructed to remove sulfamethoxazole (SMX). The BER unit powered by the stacked MFC-CWs was used as a pretreatment unit, and effluent flowed into the MFC-CW for further degradation. The experimental results indicated that the removal rate of 2 or 4 mg/L SMX in a BER unit was nearly 90%, and the total removal rate in the coupled system was over 99%. As the hydraulic retention time (HRT) was reduced from 16 h to 4 h, the SMX removal rate in the BER decreased from 75% to 48%. However, the total removal rate in the coupled system was still over 97%. The maximum SMX removal rate in the MFC-CW, which accounted for 42%-55% of the total removal, was obtained in the anode layer. In addition, the relative abundances of sul genes detected in the systems were in the order of sulI > sulII > sulIII, and significant positive correlations of sul gene copy numbers versus SMX concentration and 16S rRNA gene copy numbers were observed. Furthermore, significant negative correlations were identified between sul genes, 16S rRNA gene copy numbers, and HRT. The abundances of the sul genes in the effluent of the MFC-CW were lower than the abundances observed in the BER effluent. High-throughput sequencing revealed that the microbial community diversity of the BER was affected by running time, power supply forms and HRT. Bio-electricity from the MFC-CW may reduce microbial community diversity and contribute to reduction of the antibiotic resistance gene (ARG) abundance in the BER. Taken together, the BER-MFC-CW coupled system is a potential tool to treat wastewater containing SMX and attenuate corresponding ARG abundance.

Simulated wastewater reduced Klebsiella michiganensis strain LH-2 viability and corresponding antibiotic resistance gene abundance in bio-electrochemical reactors

A previous study revealed that the electrolytic stimulation process in bio-electrochemical reactors (BER) can accelerate growth of sulfadiazine (SDZ) antibiotic resistant bacteria (ARB) in nutrient broth medium. However, the influence of different medium nutrient richness on the fate of ARB and the relative abundance of their corresponding antibiotic resistance genes (ARGs) in this process is unknown. Specifically, it is not clear if the fate of ARB in minimal nutrition simulated wastewater is the same as in nutrient broth under electrolytic stimulation. Therefore, in this study, nutrient broth medium and the simulated wastewater were compared to identify differences in the relative abundance of Klebsiella michiganensis LH-2 ARGs in response to the electrolytic stimulation process, as well as the fate of the strain in simulated wastewater. Lower biomass, specific growth rates and viable bacterial counts were obtained in response to the application of increasing current to simulated wastewater medium. Furthermore, the percentage of ARB lethality, which was reflected by flow cytometry analysis, increased with current in the medium. A significant positive correlation of sul genes and intI gene relative abundance versus current was also observed in nutrient broth. However, a significant negative correlation was observed in simulated wastewater because of the higher metabolic burden, which may have led to decreased ARB viability. Further investigation showed that the decrease in ARGs abundance was responsible for decreased strain tolerance to SDZ in simulated wastewater. These results reveal that minimal nutrition simulated wastewater may reduce ARB and ARGs propagation in BER.