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Dive into the research topics where Yun Ji Hong is active.

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Featured researches published by Yun Ji Hong.


Tuberculosis | 2013

Simultaneous detection of Mycobacterium tuberculosis complex and nontuberculous mycobacteria in respiratory specimens

Sang Mee Hwang; Mi Suk Lim; Yun Ji Hong; Taek Soo Kim; Kyoung Un Park; Junghan Song; Jae Ho Lee; Eui Chong Kim

Many nontuberculous mycobacteria (NTM) species have clinical significance, and the rapid and reliable identification of Mycobacterium tuberculosis complex (MTBC) and NTM species is important. We evaluated the simultaneous detection of MTBC and NTM in respiratory specimens. MTBC and NTM were simultaneously detected and identified by laboratory-developed (LDT) real-time PCR, multiplex real-time PCR/melting curve analysis, rpoB PCR restriction fragment length polymorphisms and the AdvanSure Mycobacteria GenoBlot assay (LG Life Sciences). Eighty-five respiratory specimens from 69 patients showed simultaneous detection of MTBC and NTM. A line probe assay showed 70.6% concordance with LDT. Ten patients (14.5%) had a history of tuberculosis, and eight patients (11.6%) had been previously diagnosed with bronchiectasis. Mixed cultures were present one time in 57 patients (82.6%) and repeatedly in 12 patients (17.4%). MTBC was more frequent in 44 patients (63.8%), and NTM was isolated in seven patients (10.1%). The commonly detected NTM species in the mixed cultures were Mycobacterium intracellulare (29.0%) and Mycobacterium abscessus (29.0%). Co-isolation caused a failure of antitubercular drug susceptibility testing in 2 patients (2.9%). Molecular methods allow MTBC and NTM species to be simultaneously identified in respiratory specimens. NTM isolated with MTBC has clinical significance in some patients and should not be ignored.


Journal of Clinical Laboratory Analysis | 2015

Comparison of xTAG Respiratory Virus Panel and Verigene Respiratory Virus Plus for Detecting Influenza Virus and Respiratory Syncytial Virus

Sang Mee Hwang; Mi Suk Lim; Minsuk Han; Yun Ji Hong; Taek Soo Kim; Hye Ryun Lee; Eun Young Song; Kyoung Un Park; Junghan Song; Eui Chong Kim

Nucleic acid amplification tests have allowed simultaneous detection of multiple respiratory viruses.


BioMed Research International | 2014

Detection of herpes simplex and varicella-zoster virus in clinical specimens by multiplex real-time PCR and melting curve analysis.

Yun Ji Hong; Mi Suk Lim; Sang Mee Hwang; Taek Soo Kim; Kyoung Un Park; Junghan Song; Eui Chong Kim

Herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2), and varicella-zoster virus (VZV) are common agents resulting in various forms of clinical manifestation from skin vesicle to disseminated viral infection. The aim of the present study was to develop a real-time PCR and melting curve analysis which detect and differentiate HSV-1, HSV-2, and VZV, to compare with PCR-RFLP using clinical specimens, and to introduce the 4-year experience in the clinical laboratory. Three pairs of primers for HSV-1, HSV-2, and VZV were designed. Primers for human endogenous retrovirus-3 (HERV-3), an internal control, were adopted. A hundred selected specimens and many clinical specimens were tested for methods comparison and assay validation. Increased sensitivity and specificity were obtained from real-time PCR. In review of results of clinical specimens submitted to clinical laboratory, a total of 46 of 3,513 specimens were positive in cerebrospinal fluids, blood, skin vesicles, genital swabs, aqueous humor, and ear discharge. Thus, this method could be a rapid and accurate alternative to virus culture and other molecular tests for detection and typing of HSV-1, HSV-2, and VZV.


Journal of Clinical Microbiology | 2011

Usefulness of Three-Channel Multiplex Real-Time PCR and Melting Curve Analysis for Simultaneous Detection and Identification of the Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria

Yun Ji Hong; Young Hoon Chung; Taek Soo Kim; Sang Hoon Song; Kyoung Un Park; Jae Joon Yim; Junghan Song; Jae Ho Lee; Eui Chong Kim

ABSTRACT We attempted to determine the benefits of three-channel multiplex real-time PCR and melting curve analysis not only in detecting and distinguishing between nontuberculous mycobacteria (NTM) and the Mycobacterium tuberculosis complex but also in identifying NTM to the species level.


BioMed Research International | 2013

Human platelet antigen genotyping and expression of CD109 (human platelet antigen 15) mRNA in various human cell types.

Sang Mee Hwang; Mi Jung Kim; Ho Eun Chang; Yun Ji Hong; Taek Soo Kim; Eun Young Song; Kyoung Un Park; Junghan Song; Kyou-Sup Han

CD109 gene encodes a glycosylphosphatidylinositol-linked glycoprotein found in a subset of platelets and endothelial cell, and human platelet antigen (HPA) 15 is found on CD109. We evaluated the HPA genotype and/or the CD109 mRNA expression on two peripheral blood stem cells (PBSC), two peripheral bloods (PB), 12 granulocyte products, natural killer (NK)-92, B-lymphocyte (CO88BV59-1), K-562 leukemia cell line, human embryonic stem cell (hESC), and human fibroblasts (HF). HPA genotyping was performed by SNaPshot assay and CD109 mRNA expression was evaluated by real-time PCR with SYBR green and melting curve analysis. Genotype HPA-15a/-15a was found in PBSC#1 and two granulocyte products, and HPA-15a/-15b was found in PBSC#2, eight granulocyte products, NK-92, K-562, hESC, and HF, and HPA-15b/-15b was found in two granulocyte products. CD109 mRNA expression was highly increased in HF and increased in CD34+ and CD34− PBSCs and some granulocyte products, compared to the PB. However, the increase of expression level varied among the PBSC and granulocyte products. The CD109 mRNA expression of NK-92, K-562, hESC, and CO 88BV59-1 was not detected. HPA genotype was evaluated in various cells and the expression of CD109, which contains HPA 15, was different among cell lines and high in HF and PBSCs.


Annals of Hematology | 2016

Genotyping of 22 blood group antigen polymorphisms and establishing a national recipient registry in the Korean population.

Yun Ji Hong; Yousun Chung; Sang Mee Hwang; Jeong Su Park; Jeong-Ran Kwon; Young Sill Choi; Jun Nyun Kim; Dong Han Lee; So-Yong Kwon; Nam-Sun Cho; Eun Young Song; Kyoung Un Park; Junghan Song; Kyou Sup Han

It is often difficult for standard blood banks in Korea to supply adequate amounts of blood for patients with rare phenotype. Moreover, the definition of a blood in need is ambiguous, and much remains to be learned. In this study, we determined the prevalence of various red blood cell (RBC) antigens from a donor viewpoint and estimated the demand for specific antigen-negative blood from a patient viewpoint. Our data will aid the establishment of a Rare Blood Program in Korea (KRBP). RBC genotyping of 419 blood donors was performed using a Lifecodes RBC/RBC-R typing kit (Immucor, Norcross, GA). A national recipient registry website has been established. Each hospital-based blood bank voluntarily enters data on antibodies detected and identified and the outcomes of specific antigen testing. We calculated the availabilities of specific antigen-negative blood components based on these registry data and predicted the prevalence of RBC antigens via RBC genotyping. The prevalences of various RBC antigens in the D-negative population were determined for the first time, and the Cartwright, Scianna, Dombrock, Colton, Landsteiner-Wiener, Cromer, and Knops blood group systems were identified. The availabilities of specific antigen-negative units differed when calculations were based on serotyping or genotyping, especially in the D-negative group. Data on the prevalences of various blood antigens are essential for estimating the availabilities of blood components that are appropriate for use by patients expressing relevant antibodies. Then, blood banks would be able to efficiently supply safe blood products.


Korean Journal of Laboratory Medicine | 2012

A case of imported Plasmodium malariae malaria.

Yun Ji Hong; Sun Young Yang; Kyunghoon Lee; Taek Soo Kim; Hong Bin Kim; Kyoung Un Park; Junghan Song; Eui Chong Kim

Malaria, the most common vector-borne parasite infection worldwide, results from infection by Plasmodium species. Approximately 80% of malaria cases are caused by P. vivax, which is broadly distributed from tropical to temperate regions; P. falciparum is the second most common infectious species. P. malariae and P. ovale are responsible for a relatively small proportion of malaria cases. Here, we report the case of a 23-yr-old Korean woman who acquired a P. malariae infection while visiting the Republic of Ghana in West Africa for business. She was diagnosed with P. malariae malaria on the basis of peripheral blood smear (PBS) and species-specific conventional and real-time PCR assays for 18S rRNA. She was treated with hydroxychloroquine, and the resulting PBS examination on day 2 suggested that negative conversion occurred. At her 1-month follow-up, however, both the PBS examination and molecular test for malaria demonstrated recurrent parasitemia. We started rescue therapy with mefloquine, and the patient recovered successfully. This is an important finding suggesting possible late recrudescence of a chloroquine-resistant P. malariae strain identified not only by its morphological features, but also by molecular tests.


Transfusion | 2014

Proposal of standardized guidelines for the production and quality control of autologous serum eye drops in Korea: based on a nationwide survey

Hye Ryun Lee; Yun Ji Hong; Soie Chung; Sang Mee Hwang; Taek Soo Kim; Eun Young Song; Kyoung Un Park; Junghan Song; Kyou Sup Han

Autologous serum eye drops (ASEDs) have been used to treat many eye diseases. However, there are no standardized guidelines for the production and quality control (QC) of ASEDs in Korea. Our aim was to propose standardized guidelines for the production and QC of ASEDs.


international conference on micro electro mechanical systems | 2013

Multi nozzle electrohydrodynamic inkjet printing head by batch fabrication

Kyoung Il Lee; B. Lim; Hyun-Jeong Lee; Seong Hyun Kim; Churl Seung Lee; Jin Woo Cho; Sung Soo Chung; Yun Ji Hong

We report a multi nozzle electrohydrodynamic (EHD) printing head by using batch fabrication for the first time. Inks can be ejected from a glass nozzle by applied large electric field. Tiny nozzles with a outer diameter of 100 microns are fabricated by sand blasting on a glass wafer. A stable continuous jetting with a commercial carbon black ink was observed. Continuous lines with a width of 30 microns on a silicon wafer were printed simultaneously with a DC bias voltage of 1.7 kV at the stage speed of 500 mm/s. Separated dot patterns with a diameter of 20 microns were printed with various jetting frequency up to 10 kHz on a super-hydropobic teflon coated silicon wafer. Also continuous line width can be reduced to 20 microns on a hydrophobic surface.


Clinica Chimica Acta | 2016

Genotyping of 19 red cell antigens, including RHD, using liquid bead arrays

Ho Eun Chang; Yun Ji Hong; Hyung Suk Kim; Sang Mee Hwang; Jeong Su Park; Seong-Wook Lee; Eun Young Song; Kyoung Un Park; Junghan Song; Kyou-Sup Han

BACKGROUND Traditionally, the determination of blood group has been performed using serological techniques. Due to the drawbacks of using antisera, molecular typing of blood groups has been introduced. However, the commonly used genotyping assays in blood banks have limitations because the panels for these were based on genetic variations in Caucasians. METHODS We developed a multiplex human erythrocyte antigen genotyping assay using allele-specific primer extensions and hybridization with bead microarrays. Single nucleotide polymorphisms (SNP) were genotyped for the 18 red cell antigens and wild-type RHD, DEL, and complete deletion were examined for RHD. The cut-off of median fluorescence intensity (MFI) for each allele was optimized. In the evaluation stage, the results of 87 samples were compared with those from other genotyping methods, and 26 serologic results were also compared. RESULTS The cut-off values were determined using -1 and -2 standard deviation (SD) of the minimum adjusted MFI for SNP detection. Complete deletion was determined with raw MFI+2SD. In comparison with the other methods, the kappa values were 0.984 overall. Compared with serologic methods, our assay showed discrepancy in 2 S/s, 3 C/c, and 3 Dia/Dib antigens. CONCLUSIONS Our method reliably predicts the presence or the absence of the 19 antigens.

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Kyoung Un Park

Seoul National University Bundang Hospital

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Junghan Song

Seoul National University Bundang Hospital

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Sang Mee Hwang

Seoul National University

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Taek Soo Kim

Seoul National University

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Eun Young Song

Seoul National University

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Eui Chong Kim

Seoul National University

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Jeong Su Park

Seoul National University

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Kyou Sup Han

Seoul National University

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Kyou-Sup Han

Seoul National University

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Hyung Suk Kim

Seoul National University Hospital

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