Yun-Zhang Sun

Prebiotics and Fish Immune Response: A Review of Current Knowledge and Future Perspectives

It is now a well-documented fact that there is a distinct relation between fish nutrition and health status. Intensification of aquaculture practice to meet market demand causes stress and elevates the risk of the disease outbreak. Therefore, provisions of proper diets as well as appropriate feeding regimens are of high importance in intensive aquaculture. Considering the negative impacts raised by prophylactic and therapeutic use of antibiotic in aquaculture, administration of dietary immunostimulant has been suggested as an alternative to antimicrobial agents. In this sense, functional dietary supplements, including pre-, pro- and synbiotic received increasing attention as an environment-friendly strategy for improving fish health. During the past years, administration of prebiotics in the diet of different fish species revealed promising results in immune response. In the present review article, the topic of the administration of dietary prebiotics in aquaculture is addressed with a special focus on recent findings regarding the effects of prebiotics on fish immune response and possible mode of action. Furthermore, the present study covers the gap in existing knowledge and suggests issues that merit further investigation.

Probiotic Bacillus pumilus SE5 shapes the intestinal microbiota and mucosal immunity in grouper Epinephelus coioides

The health benefits of probiotics are thought to occur, at least in part, through an improved intestinal microbial balance in fish, although the molecular mechanisms whereby probiotics modulate the intestinal microbiota by means of activation of mucosal immunity are rarely explored. In this study, the effects of viable and heat-inactivated probiotic Bacillus pumilus SE5 on the intestinal dominant microbial community and mucosal immune gene expression were evaluated. The fish were fed for 60 d with 3 different diets: control (without probiotic), and diets T1 and T2 supplemented with 1.0 × 10⁸ cells g⁻¹ viable and heat-inactivated B. pumilus SE5, respectively. Upregulated expression of TLR1, TLR2 and IL-8, but not MyD88 was observed in fish fed the viable probiotic, while elevated expression of TLR2, IL-8 and TGF-β1, but not MyD88 was observed in fish fed the heat-inactivated B. pumilus SE5. The induced activation of intestinal mucosal immunity, especially the enhanced expression of antibacterial epinecidin-1, was consistent with the microbial data showing that several potentially pathogenic bacterial species such as Psychroserpens burtonensis and Pantoea agglomerans were suppressed by both the viable and heat-inactivated probiotic B. pumilus SE5. These results lay the foundation for future studies on the molecular interactions between probiotics, intestinal microbiota and mucosal immunity in fish.

Probiotics as Means of Diseases Control in Aquaculture, a Review of Current Knowledge and Future Perspectives

Along with the intensification of culture systems to meet the increasing global demands, there was an elevated risk for diseases outbreak and substantial loss for farmers. In view of several drawbacks caused by prophylactic administration of antibiotics, strict regulations have been established to ban or minimize their application in aquaculture. As an alternative to antibiotics, dietary administration of feed additives has received increasing attention during the past three decades. Probiotics, prebiotics, synbiotics and medicinal plants were among the most promising feed supplements for control or treatments of bacterial, viral and parasitic diseases of fish and shellfish. The present review summarizes and discusses the topic of potential application of probiotics as a means of disease control with comprehensive look at the available literature. The possible mode of action of probiotics (Strengthening immune response, competition for binding sites, production of antibacterial substances, and competition for nutrients) in providing protection against diseases is described. Besides, we have classified different pathogens and separately described the effects of probiotics as protective strategy. Furthermore, we have addressed the gaps of existing knowledge as well as the topics that merit further investigations. Overall, the present review paper revealed potential of different probiont to be used as protective agent against various pathogens.