Yuqin Shen

The Influence of Processing Parameters on the Mechanical Properties of Selectively Laser Melted Stainless Steel Microlattice Structures

The rapid manufacturing process of selective laser melting has been used to produce a series of stainless steel 316L microlattice structures. Laser power and laser exposure time are the two processing parameters used for manufacturing the lattice structures and, therefore, control the quality and mechanical properties of microlattice parts. An evaluation of the lattice material was undertaken by manufacturing a range of struts, representative of the individual trusses of the microlattices, as well as, microlattice block structures. Low laser powers were shown to result in significantly lower strand strengths due to the presence of inclusions of unmelted powder in the strut cross-sections. Higher laser powers resulted in struts that were near to full density as the measured strengths were comparable to the bulk 316L values. Uniaxial compression tests on microlattice blocks highlighted the effect of manufacturing parameters on the mechanical properties of these structures and a linear relationship was found between the plateau stress and elastic modulus relative to the measured relative density.

The Mechanical Properties of Sandwich Structures Based on Metal Lattice Architectures

A range of metallic lattice structures were manufactured using the selective laser melting (SLM) rapid prototyping technique. The lattices were based assemblies of repeating unit-cells with their strands oriented at 0°, ±45°, and 90° to the vertical when viewed from the front. Mechanical tests on the strands and the lattice blocks showed that these systems exhibit a high level of reproducibility in terms of their basic mechanical properties. An examination of the compression failure mechanisms showed that the [±45°] and [±45°, 90°] lattices failed in bending and stretching modes of failure, whereas the [0°, ±45°] lattices failed as a result of buckling of the vertical pillars. Sandwich structures were manufactured by binding woven carbon-fiber reinforced plastic to the lattice structures. Subsequent three-point bend tests on these structures identified the principal failure mechanisms under flexural loading conditions. Here, cell crushing, hinge rotation, and gross plastic deformation in the strands were observed directly under the point of loading. Low-velocity impact tests were conducted on the sandwich beams and a simple energy-balance model was used to understand how energy is absorbed by the sandwich structures. The model suggests that the majority of the incident energy of the projectile was absorbed in indentation effects, predominantly in the core material, directly under the steel indenter.

Geometrical effects in the impact response of the aluminium honeycomb sandwich structures

Low-velocity impact tests have been conducted on sandwich beams and panels based on [0°, 90°] glass fibre-reinforced epoxy skins with an aluminium honeycomb core. The sandwich beams were placed on simple supports positioned at different separations to investigate the effect of target size on their impact response. Two thicknesses (13 and 26 mm) of honeycomb core were investigated. Following impact, damage within the beams was assessed by sectioning the samples and observing them under a low-power microscope. An energy-balance model was used to predict the maximum impact force for subsequent comparison with the experimental results. The energy-balance model was used to partition the energy absorbed during the impact event, giving an understanding of the energy absorption process. Finally, a limited number of tests have been conducted on square sandwich panels in order to investigate the effect of impact loading on their indentation behaviour. An examination of the cross-sections of impacted samples highlighted regions of core buckling and local plastic folding. At higher energies, damage in the form of localized fibre fracture was observed in the top skin close to the point of impact. The energy-balance model predicted the elastic response of the sandwich beams with reasonable success. The accuracy of the model decreased as damage became more extensive in the beams. It has been shown that the level of permanent indentation in both the beams and the square panels collapsed onto a single curve when the data are plotted against maximum impact force.

VE/VCO2 slope and its prognostic value in patients with chronic heart failure.

The minute ventilation/carbon dioxide production (VE/VCO2) slope has been widely demonstrated to have strong prognostic value in patients with chronic heart failure (CHF), and the risk of mortality is believed to increase when the VE/VCO2 slope is >32.8; however, there is little evidence concerning the prognostic value of the VE/VCO2 slope in Chinese patients. In the present study, the prognostic value of the VE/VCO2 slope was investigated in patients with CHF. A total of 258 subjects underwent symptom-limited cardiopulmonary exercise testing (CPET) and were divided into CHF (113 males and 16 females; LVEF <0.49) and control (106 males and 23 females) groups. The cardiac-related events over a median 33.7-month follow-up period subsequent to the CPET were evaluated using receiver operating characteristic curve analysis. The VE/VCO2 slope was significantly different between the CHF and control groups (P<0.001). The area under the curve (AUC) for the VE/VCO2 slope in predicting cardiac-related mortalities in the patients with CHF was 0.670 (P<0.05), and the sensitivity and specificity of the VE/VCO2 slope were 0.667 and 0.620, respectively. The optimal threshold of the VE/VCO2 slope for predicting cardiac-related mortalities in patients with CHF was ≥39.3. The AUC for the VE/VCO2 slope in predicting cardiac-related hospitalizations in patients with CHF was 0.682 (P<0.05), and the sensitivity and specificity of the VE/VCO2 slope were 0.631 and 0.778, respectively. The optimal threshold of the VE/VCO2 slope for predicting cardiac-related hospitalizations in patients with CHF was ≥32.9. In conclusion, ventilatory efficiency decreases in patients with CHF. The VE/VCO2 slope is a strong predictor of cardiac-related mortalities in the patients with CHF analyzed.

Immune Cell Repertoire and Their Mediators in Patients with Acute Myocardial Infarction or Stable Angina Pectoris

Background: To evaluate the natural innate and adaptive immunity through gene expression and cytology levels in peripheral blood mononuclear cells in patients with acute myocardial infarction (AMI), stable angina pectoris (SAP) and controls. Methods: 210 patients with AMI, 210 with SAP, and 250 clinical controls were recruited. Whole human genome microarray analysis was performed in 20 randomly chosen subjects per group were examined to detect the expressions of complement markers, natural killer cells, T cells and B cells. The quantity of these cells and related cytokines as well as immunoglobulin levels were measured in all subjects. Results: In AMI group, the mRNA expressions of late complement component, markers of natural killer cells, CD3+, CD8+ T cells and B cells were down-regulated, while those of early complement component and CD4+T cells were up-regulated (p<0.05). In both AMI and SAP patients, the quantity of natural killer cells, CD3+, CD8+ T cells, B cells, IgM and IgG were significantly lower than those of the controls. CD4+ T cells, CH50, C3, C4, IL-2, IL-4, IL-6 and IFN-γ were significantly higher (p<0.05). Conclusions: In AMI patients, both of gene expressions related to complement, natural killer cells, CD3+, CD8+ T cells, B cells and the quantity of these immune cells decreased while cell number reduced in SAP patients. Immune function in both AMI and SAP patients decreased especially in AMI patients with declined gene and protein levels. To improve the immune system is a potential target for medical interventions and prevention in AMI.

The Prognostic Value of Peak Cardiac Power Output in Chinese Patients with Chronic Heart Failure.

Background Cardiopulmonary exercise testing has been widely used to risk stratify patients with chronic heart failure (CHF). Peak oxygen consumption (peakVO2) was regarded as a powerful predictor of survival, as it is a surrogate for peak cardiac output (CO), which by most is considered the “true” measure of heart failure. Therefore, it is reasonable to hypothesize that CO is an even stronger predictor than peak VO2. The present study is aimed to investigate the prognostic value of peak cardiac power output (peak CPO) in comparison with peakVO2 in Chinese patients with CHF. Methods Participants provided written informed consent to participate in this study. Totally 129 patients with CHF underwent symptom-limited cardiopulmonary exercise testing (CPET), with mean age 59.1±11.4 years, 87.6% male, 57.4% ischemic etiology, body mass index (BMI) 24.7±3.7 kg/m2 and LVEF 38±9%. CO was measured using an inert gas rebreathing method. The primary endpoints are cardiac deaths. Results Over median 33.7-month follow-up, 19 cardiac deaths were reported. Among peak VO2,VE/VCO2 slope and Peak CPO, their area under ROC were 0.64, 0.67, 0.68, respectively (Ρ<0.05).The optimal thresholds for predicting cardiac deaths were peak VO2≤13.4 ml.kg-1.min-1, and VE/VCO2 slope≥39.3 and peak CPO≤ 1.1 respectively by ROC analysis. Finally, in patients with a peak VO2≤13.4 ml.kg-1.min-1 those with peak CPO>1.1W had better survival than those with peak CPO ≤ 1.1W. However, by multivariate analysis adjusted for age, sex, BMI, resting heart rate, LVMI, LVEF, Peak CPO was not an independent predictor of cardiac deaths (P> 0.05). Conclusions Peak CPO was not a predictor of cardiac death in Chinese CHF patients.

Effect of Aerobic Exercise on Treg and Th17 of Rats with Ischemic Cardiomyopathy

Immune activation and inflammation participate in the progression of chronic heart failure (CHF). Th17 cells and CD4+CD25+ regulatory T (Treg) cells both come from naive Th cells and share reciprocal development pathways but exhibit opposite effects. We hypothesized that the Th17/Treg balance was impaired in patients with CHF, and exercise can improve it. Rats with ischemic cardiomyopathy were prepared by ligaturing the left anterior descending branch of the left coronary artery. Rats in training group were trained with treadmill; Th17 cells increased significantly while Treg cells significantly decreased in s by flow cytometry, and the peripheral blood level of IL-6, IL-17, and TNF-α was obviously elevated by ELISA assay. We found that Th17/Treg balance is impaired in CHF rats, suggesting Th17/Treg imbalance potentially plays a role in the pathogenesis of CHF. Exercise can improve Th17/Treg imbalance, which also improves cardiac function of CHF.