Yusuke Kimura

Influence of multinerve-sparing, robot-assisted radical prostatectomy on the recovery of erection in Japanese patients

To evaluate in Japanese patients their sexual function after robot‐assisted radical prostatectomy (RARP) and to investigate the influence of the multinerve‐sparing (NS) grade on their sexual function.

MP94-08 GROUP III METABOTROPIC GLUTAMATE RECEPTOR-MEDIATED REGULATION OF MICTURITION REFLEX IN URETHANE-ANESTHETIZED RATS

analysis BMI (OR: 1.28 per unit, 95%CI: 1.04-1.58; p 1⁄4 0.019) and severe nocturia (6.26, 95%CI: 1.71-22.92, p 1⁄4 0.006) were independent predictive factors for NP, while only severe nocturia (3.77, 95%CI: 1.2011.83, p 1⁄4 0.023) was an independent predictive factor for a reduced BC. CONCLUSIONS: Patients with NP presented a different BC between day-time and night-time. Severe nocturia ( 3 episodes per night) predicts the presence of NP and a reduced BC. Our data suggest that in patients with severe nocturia both conditions should be considered and managed.

MP97-18 LONGITUDINAL HEALTH-RELATED QUALITY OF LIFE AFTER ROBOT-ASSISTED RADICAL PROSTATECTOMY

The IH recurrence rate was lower in the IPOM group by a clinically relevant but not statistically significant difference (4% vs. 9%; p1⁄40.58) CONCLUSIONS: In patients undergoing RALP and IH repair, IPOM repair is feasible, and may be associated with better perioperative outcomes and safety profile than suture repair. The effectiveness of IPOM repair was superior but not significantly. A prospective comparison against the gold-standard technique is warranted.

MP07-15 ALTERATIONS IN OXIDATIVE STRESS PARAMETERS IN THE TESTIS AND EPIDIDYMIS IN A NICOTINE-EXPOSED RAT MODEL. CAN NICOTINE-ABSTINENCE OVERCOME THE OXIDATIVE DAMAGE?

INTRODUCTION AND OBJECTIVES: Voltage-dependent anion channel (VDAC), a channel protein, exists in the outer mitochondrial membrane of somatic cells and is involved in multiple physiological and pathophysiological processes. Up until now, little has been known about VDAC in male germ cells. In the present study, the relationship between VDAC and human sperm motility was explored. METHODS: Highly motile human spermatozoa were incubated in vitro with anti-VDAC antibody. Total sperm motility, straight line velocity (VSL), curvilinear velocity (VCL), and average path velocity (VAP) were recorded. Intracellular free calcium concentration ([Ca2þ]i), pH value (pHi), and ATP content were determined. RESULTS: Co-incubation with anti-VDAC antibody reduced VSL, VCL, and VAP of spermatozoa. Co-incubation further reduced [Ca2þ]i. Anti-VDAC antibody did not significantly alter total sperm motility, pHi and intracellular ATP content. CONCLUSIONS: The data suggest that co-incubation with antiVDAC antibody reduces sperm motility through inhibition of Ca2þ transmembrane flow. In this way, VDAC participates in the modulation of human sperm motility through mediating Ca2þ transmembrane transport and exchange.

MP94-16 OXIDATIVE STRESS-RELATED ALTERATIONS IN THE BLADDER OF A SHORT-PERIOD DIABETES TYPE-2 RAT MODEL

INTRODUCTION AND OBJECTIVES: Bladder smooth muscle (BSM) caveolae are cholesterol-enriched membrane microdomains that augment or attenuate detrusor functional responses by differentially regulating specific receptor-activated signaling pathways. However, BSM contractions induced by cholinergic activation in the rat are not altered by depletion of caveolae, unlike other smooth muscle systems in which muscarinic receptor signaling is evidently mediated by caveolae. Although this discrepancy may reflect the highly specific regulation imparted by caveolae among different tissues and species, a differential regulation of muscarinic M3 and M2 receptor subtypes in the bladder cannot be excluded. This study examined the functional and molecular relationship between caveolae and muscarinic acetylcholine receptor (mAChR) subtypes M3 and M2. METHODS: BSM tissue strips were prepared from Sprague Dawley rat bladders after removing the mucosa. Tissue was suspended in organ baths for isometric tension studies. Dose response curves to carbachol (CCh, 1nM-10mM) were generated at baseline, as well as in the presence of 4-DAMP (10nM) or AFDX (0.1mM) to inactive M3 or M2 receptors respectively. Responses to CCh were repeated after incubation with methyl-b-cyclodextrin (mbCD, 15mM), an agent that disrupts caveolae by depleting membrane cholesterol. Interaction between caveolin-1 (Cav-1, a protein required for caveolae biogenesis) and M3 or M2 mAChR subtype were investigated by co-immunoprecipitation. RESULTS: Compared to baseline responses, 4-DAMP decreased CCh-induced contractions at each dose. After mbCD treatment and in the presence of 4-DAMP, contractile responses to CCh were significantly enhanced. AFDX had little effect on CCh doseresponse curves. However, the subsequent disruption of caveolae in the presence of AFDX attenuated significantly contractions induced by CCh. Immunoreactive bands corresponding to M3 and M2 mAChR subtypes were detected in Cav-1 immunoprecipitates. CONCLUSIONS: The opposite effect of mbCD on CCh responses in the presence of M2 or M3 antagonists suggests that caveolae negatively regulate M2and positively regulate M3-mediated signaling respectively, but this interaction is masked when only the aggregate effect of CCh is examined. Molecular interaction of Cav-1 and mAChRs is consistent with their localization within caveolae. Changes in the balance among caveolin-mAChR interactions, due to loss of caveolae or changes in mAChR subtype expression, may alter responses to cholinergic activation or the efficacy of anti-muscarinic agents in the bladder.