Yusuke Yamagishi
University of Tokyo
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Featured researches published by Yusuke Yamagishi.
ACS Chemical Biology | 2008
Yuki Goto; Atsushi Ohta; Yusuke Sako; Yusuke Yamagishi; Hiroshi Murakami; Hiroaki Suga
The initiation codon dictates that the translation initiation event exclusively begins with methionine. We report here a new technology to reprogram the initiation event, where various amino acids and those bearing N (alpha)-acyl groups can be used as an initiator for peptide synthesis. The technology is built upon the concept of genetic code reprogramming, where methionine is depleted from the translation system and the initiation codon is reassigned to the desired amino acid. We have applied this technology to the synthesis of an antitumor cyclic peptide, G7-18NATE, closed by a physiologically stable bond, and it is also extended to the custom synthesis of its analogues with various ring sizes. Significantly, cyclization occurs spontaneously upon translation of the precursor linear peptides. To demonstrate the practicality of this methodology, we also prepared a small cyclic peptide library designated by 160 distinct mRNAs. Thus, this technology offers a new means to prepare a wide array of in vivo compatible cyclic peptide libraries for the discovery of peptidic drug candidates against various therapeutic targets.
Bioorganic & Medicinal Chemistry Letters | 2009
Nobuyoshi Niwa; Yusuke Yamagishi; Hiroshi Murakami; Hiroaki Suga
We have developed a new flexizyme (a flexible de novo tRNA acylation ribozyme) system, a pair of amino-derivatized benzyl thioester (ABT) and amino flexizyme (aFx). ABT bearing the ammonium ion was designed to render the acyl-donor substrates better water solubility. Although the previously reported flexizymes (eFx and dFx) did not show acylation activity for the ABT derivatives, a new flexizyme variant aFx, generated by in vitro selection against an amino acid activated ABT, exhibits high selectivity toward those activated ABT. The flexizymes system including aFx, eFx, and dFx enables us to prepare a wide variety of acyl-tRNAs charged with non-proteinogenic amino acids.
ChemBioChem | 2009
Yusuke Yamagishi; Hiroshi Ashigai; Yuki Goto; Hiroshi Murakami; Hiroaki Suga
Ring around the peptides: We demonstrate a new method for the cyclization of peptides that involves the oxidative coupling of 5‐hydroxyindole and benzylamine. After two nonproteinogenic amino acids were incorporated into peptides by reprogramming the genetic code, cyclization took place rapidly upon the addition of K3Fe(CN)6 and generated a conjugated, fluorescent, heterocyclic structure.
Chemistry & Biology | 2011
Yusuke Yamagishi; Ikuo Shoji; Shoji Miyagawa; Takashi Kawakami; Takayuki Katoh; Yuki Goto; Hiroaki Suga
Current Opinion in Chemical Biology | 2008
Atsushi Ohta; Yusuke Yamagishi; Hiroaki Suga
Archive | 2008
Hiroaki Suga; Hiroshi Murakami; Yuki Goto; Yusuke Yamagishi; Hiroshi Ashigai; Yusuke Sako
Archive | 2011
Hiroaki Suga; Yusuke Yamagishi
Archive | 2011
Hiroaki Suga; Yusuke Yamagishi
Archive | 2012
Shiori Kariyuki; Takeo Iida; Miki Kojima; Ryuichi Takeyama; Mikimasa Tanada; Tetsuo Kojima; Hitoshi Iikura; Atsushi Matsuo; Takuya Shiraishi; Takashi Emura; Kazuhiko Nakano; Koji Takano; Kousuke Asou; Takuya Torizawa; Ryusuke Takano; Nozomi Hisada; Naoaki Murao; Atsushi Ohta; Kaori Kimura; Yusuke Yamagishi; Tatsuya Kato
Peptide science : proceedings of the ... Japanese Peptide Symposium | 2013
Naohisa Murakami; Yuki Goto; Yusuke Yamagishi; Takayuki Katoh; William P. Bozza; Kun Yang; Zhihao Zhuang; Hiroaki Suga