Yuya Asanomi
Hokkaido University
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Publication
Featured researches published by Yuya Asanomi.
Journal of Medicinal Chemistry | 2010
Julian Nomme; Axelle Renodon-Cornière; Yuya Asanomi; Kazuyasu Sakaguchi; Alicja Z. Stasiak; Andrzej Stasiak; Bengt Nordén; Vinh Tran; Masayuki Takahashi
We have previously shown that a 28-amino acid peptide derived from the BRC4 motif of BRCA2 tumor suppressor inhibits selectively human RAD51 recombinase (HsRad51). With the aim of designing better inhibitors for cancer treatment, we combined an in silico docking approach with in vitro biochemical testing to construct a highly efficient chimera peptide from eight existing human BRC motifs. We built a molecular model of all BRC motifs complexed with HsRad51 based on the crystal structure of the BRC4 motif-HsRad51 complex, computed the interaction energy of each residue in each BRC motif, and selected the best amino acid residue at each binding position. This analysis enabled us to propose four amino acid substitutions in the BRC4 motif. Three of these increased the inhibitory effect in vitro, and this effect was found to be additive. We thus obtained a peptide that is about 10 times more efficient in inhibiting HsRad51−ssDNA complex formation than the original peptide.
Protein and Peptide Letters | 2010
Yuya Asanomi; Yumiko Kobayashi; Hiroki Sakai; Takuya Masuda; Xinjiang Chen; Yoshiro Chuman; Kohei Uosaki; Kazuyasu Sakaguchi
We report that the addition of amino acids to the amyloid peptide dramatically affected the structure and the rate of formation of amyloid fibrils. The attachment of three lysines to Abeta(10-35) gave the formation of remarkably long fibrils, while three glutamates resulted in a faster formation rate of the fibrils.
Biochemistry | 2006
Yuichiro Higashimoto; Yuya Asanomi; Satoru Takakusagi; Marc S. Lewis; Kohei Uosaki; Stewart R. Durell; Carl W. Anderson; Ettore Appella; Kazuyasu Sakaguchi
Advanced Functional Materials | 2013
Hiroki Sakai; Ken Watanabe; Yuya Asanomi; Yumiko Kobayashi; Yoshiro Chuman; Lihong Shi; Takuya Masuda; Thomas Wyttenbach; Michael T. Bowers; Kohei Uosaki; Kazuyasu Sakaguchi
Advanced Functional Materials | 2013
Hiroki Sakai; Ken Watanabe; Yuya Asanomi; Yumiko Kobayashi; Yoshiro Chuman; Lihong Shi; Takuya Masuda; Thomas Wyttenbach; Michael T. Bowers; Kohei Uosaki; Kazuyasu Sakaguchi
Peptide science : proceedings of the ... Japanese Peptide Symposium | 2009
Hiroki Sakai; Yuya Asanomi; Yumiko Kobayashi; Xinjiang Chen; Yoshiro Chuman; Takuya Masuda; Kohei Uosaki; Kazuyasu Sakaguchi
Bulletin of the American Physical Society | 2008
Kohei Uosaki; Masaya Tsukamoto; Kazuyasu Sakaguchi; Yuya Asanomi
Peptide science : proceedings of the ... Japanese Peptide Symposium | 2006
Yuya Asanomi; Yuichiro Higashimoto; Yoshiro Chuman; Toshiaki Imagawa; Kazuyasu Sakaguchi
Peptide science : proceedings of the ... Japanese Peptide Symposium | 2005
Yuya Asanomi; Satoru Takakusagi; Yoshiro Chuman; Toshiaki Imagawa; Kohei Uosaki; Kazuyasu Sakaguchi
Peptide science : proceedings of the ... Japanese Peptide Symposium | 2004
Yuichiro Higashimoto; Yuya Asanomi; S Lewis Marc; Ettore Appella; Kazuyasu Sakaguchi