Yuzuru Otsuka

Purification of new calcium activated protease (low calcium requiring form) and comparison to high calcium requiring form

A new calcium activated protease which requires low concentration of calcium was purified to almost homogeneity from porcine heart muscle. The protease was composed of two polypeptide chains of approximately 90 K and 30 K. The 90 K subunit was larger than the large subunit of the high calcium requiring form of calcium activated protease, therefore we concluded that the low calcium requiring form is different from the high calcium requiring form and its auto-digested protease. The low calcium requiring form of calcium activated protease was also activated by manganese and balium, and was very stable even at pH 9.0.

Phytotoxins and related metabolites produced by Bipolaris coicis, the pathogen of job's tears

Abstract Four metabolites were isolated and characterized from the phytopathogenic fungus Bipolaris coicis H-13-3. Radicinin and its diastereomer were phytotoxic against Jobs tears ( Coix lachryma-jobi ), a host of this fungus, but a diastereomer of radicinol and its epoxide were not phytotoxic.

Change in the Contents of Arginine, Ornithine, and Urea in the Muscle of Marine Invertebrates Stored in Ice

To investigate the usefulness of ornithine and urea as freshness indices for marine invertebrates, the muscle tissue from freshly caught samples was stored in ice for 15 days and the free amino acid concentration was measured. The total amount of free amino acids decreased slightly after 1 day in most of the samples. The arginine concentration also decreased in most of the samples, especially in kuro shrimp during ice storage, while the ornithine and urea levels increased markedly in arrow squid and kuro shrimp, and increased considerably in kuruma prawn. Based on these results, it is proposed to use urea and ornithine as freshness indices for kuro shrimp and arrow squid, while only urea is proposed for kuruma prawn.

Response of anti-oxidant enzymes mRNA in the neonatal rat liver exposed to 1,2,3,4-tetrachlorodibenzo-p-dioxin via lactation.

Background : The aim of this study was to assess the response to dioxin‐induced oxidative stress in neonates via lactation in the model we have described previously.

Nutritional status of the people living in Sajison and Iwamicho-Tajiri of Tottori Prefecture.

個人別栄養素摂取量を明らかにするために栄養調査を行ない, 食品類別荷重平均成分表ではなく, 四訂版食品標準成分表を用いて個人別摂取量を計算した。その結果, 集団レベルでの摂取量は栄養所要量を充足しているが, 個人別栄養摂取量にはばらつきが大きく, とくにビタミンA, Dに摂取量の差が大きかった。食品群別摂取量をもとに計算した場合ビタミンA等の摂取量が低くなった。