Yves Denoux

Proliferative activity in primary breast carcinomas is a salient prognostic factor

The goal of the current study was to investigate the prognostic impact of proliferative activity, together with the other classic clinicopathologic prognostic factors (tumor size, tumor grade, receptor status, ploidy, and lymph node status), in breast carcinoma by counting mitoses and evaluating S phase fraction (SPF) in fresh and frozen tumor samples.

Expression of α V-associated integrin β subunits in epithelial ovarian cancer andits relation to prognosis in patients treated with platinum-based regimens

AbstractThe aim of the study was to investigate the relationships between the expression of αv, β1, β3, β5, and β6, integrin subunits and clinical parameters in ovarian cancers. Ovarian surface epithelium (OSE) from five donors and tumour samples from 39 patients with an epithelial ovarian cancer (39 primary tumours and 21 associated peritoneal metastases) were analysed using immunohistochemistry on paraffin-embedded or frozen tissue sections. The αv and β5 integrin subunits were always present in normal OSE and in tumours. β1 and β3 subunit expression was significantly less frequent in grade 3 than in grade 1–2 tumours. The proportion of stage IV tumours expressing β3 was significantly lower as compared to other stages. The β6 subunit was undetectable in OSE but was expressed in about 40% of primary tumours. For all integrin, there was a strong relationship between the expression in primary tumours and in associated peritoneal metastases. Survival analyses restricted to patients receiving platinum-based chemotherapy did not reveal any relationship between integrin subunit expression and 3-year survival rate, in this limited series of patients. In conclusion, the expression of the various β integrin subunits was differentially altered in ovarian carcinoma, evocative of complementary roles of αv integrins during tumour development.

Prognostic value of DNA cytometry in 281 premenopausal patients with lymph node negative breast carcinoma randomized in a control trial

The clinical relevance of DNA image cytometry (ICM) and flow cytometry (FCM) remains under investigation in breast carcinoma. The objective of the current work was to study the prognostic value of DNA ICM and FCM in a series of patients randomized in a control trial. A multivariate analysis has been performed including other factors still under investigation such as Ki‐67 index, mitotic count, microvessel density, and P53 and Bcl‐2 expression.

Flow cytometry in primary breast carcinomas: Prognostic impact of multiploidy and hypoploidy

The aims of the present work were to study the prognostic impact of multiploidy and/or hypoploidy in breast cancers and their relation to other classic clinicopathologic prognostic factors (T, grade, receptors, and lymph node status).

Immunohistochemical study of cell cycle regulatory proteins in intraductal breast carcinomas—a preliminary study

The aim of this study was to assess the levels of cell cycle regulatory proteins p21waf1 (p21), p53, Cyclin A, Cyclin D1 and Ki-67 to see whether they correlated with recurrence-free survival (RFS). From 1982 to 1996, 50 patients aged less than 51 years underwent lumpectomy followed by radiotherapy for a pure ductal carcinoma in situ (DCIS). For each case, the following immunohistochemical stains were carried out: Ki-67, Cyclin A, Cyclin D1, p53 and p21waf1 (p21). The percentage of positive nuclei was assessed. Multiple combinations of these factors were performed; in particular, we called the sum of Ki-67 and Cyclin A a global proliferation factor (GPF). Correlations with classical clinicopathological data were assessed. After a multivariate analysis, only GPF, Van Nuys Prognostic Index (VNPI) grade and mitotic index were independent predictive factors of recurrence in the whole population. In the population with close surgical margins, when the GPF level was less than the 25th percentile or more than the 75th percentile recurrence was low. In this preliminary study, GPF seems to be of interest to help in the decision process in the post-surgical management of the patient.

Macrobiopsies assistées par le vide : expérience du CRLCC François Baclesse de Caen

Resume Objectif Rapporter une experience homogene unicentrique des macrobiopsies realisees au Mammotome sous stereotaxie. Materiel et methode Notre serie retrospective a inclus 248 procedures chez 236 patientes, pour des foyers de microcalcifications dans 95 % des cas, chez lesquelles on ete realise une macrobiopsie sous Mammotome pour des lesions classees ACR3, ACR4 et ACR5 dans respectivement 8,4 %, 81,6 % et 14,8 p des cas. Resultats D’un point de vue technique, 91 % des procedures n’ont pas pose de problemes. L’exerese radiologique a ete totale dans 68 % des foyers infracentimetriques et dans 6 % des foyers supracentimetriques. La sous estimation des carcinomes canalaires in situ versus carcinomes infiltrants a ete de 27 % tandis que la sous estimation des hyperplasies canalaires atypiques versus carcinomes canalaires in situ a ete de 25 % en sachant que les hyperplasies canalaires atypiques dont l’exerese etait complete radiologiquement et sans facteur de risque associe n’ont pas ete operees. Conclusion Meme si la macrobiopsie des foyers de microcalcifications est une procedure efficace, sa principale limite reside dans les risques de sous estimation des lesions frontieres qui reste significatif.