Zafer Mert

Chromosomal location of genomic SSR markers associated with yellow rust resistance in Turkish bread wheat (Triticum aestivum L.)

We have previously reported Xgwm382 as a diagnostic marker for disease resistance against yellow rust in Izgi2001 × ES14 F2 population. Among the same earlier tested 230 primers, one SSR marker (Xgwm311) also amplified a fragment which is present in the resistant parent and in the resistant bulks, but absent in the susceptible parent and in the susceptible bulks. To understand the chromosome group location of these diagnostic markers, Xgwm382 and Xgwm311, in the same population, we selected 16 SSR markers mapped only in one genome of chromosome group 2 around 1–21xa0cM distance to these diagnostic markers based on the SSR consensus map of wheat. Out of 16 SSRs, Xwmc658 identified resistant F2 individuals as a diagnostic marker for yellow rust disease and provided the location of Xgwm382 and Xgwm311 on chromosome 2AL in our plant material.

Proteome profiling of the compatible interaction between wheat and stripe rust

Over the last decade, comparative molecular profiling studies between compatible and incompatible plant-pathogen interactions have shown that susceptible response of the host to a pathogen requires factors that promote disease development. In this study, we examined proteome profiles during a compatible interaction between wheat and stripe rust. A 2D-LC system (ProteomeLab PF2D) was used for protein separation and to compare the proteome from infected and control samples. More than 700 protein peaks at each time point were compared between pathogen- and mock-inoculated samples. Selected proteins, with significant differences in abundance were identified by nanoLC-ESI- MS/MS and generated spectra were searched against the wheat protein databases from UniProt, and NCBI and the Puccinia database from The Broad Institute. In total, the identified proteins comprised of 62xa0% wheat and 38xa0% Pst proteins. All identified proteins were searched by bioinformatics-based algorithms to detect their subcellular localization and signal peptide motifs which have the potential to catch the candidate effector proteins. The wheat proteins were classified based on their function. Although a compatible interaction, many wheat proteins, such as antioxidants, PRs and cold-responsive proteins, are implicated in defense and stress tolerance. On the pathogen side, 64 proteins were identified, and included some important pathogenicity proteins that can play role in pathogen virulence and suppress the host defense. In addition, we discovered that nine proteins have a signal sequence and three of the hypothetical fungal proteins, PGTG_11681T0, PGTG_07231T0 and CBH50687.1, have been tentatively identified as candidate effectors.

Mercimek (Lens culinaris M.) Germplasmında Herbisit Toleransı için Genetik Çeşitliliğin Belirlenmesi

Mercimegin verimini ve uretimini sinirlayan en onemli faktorlerden biri yabanci otlardir. Bircok yil yabanci otlar mercimek veriminde %100’e varan kayiplara neden olmaktadir. Ozellikle Ic Anadolu Bolgesinde kislik uretimin onundeki en onemli engel yabanci otlardir. Son yillarda dunyada Imidazolinone (IMI) grubu herbisitlere dayanikli mercimekler gelistirilmis ve ciftcinin kullanimina sunulmustur. Calismanin amaci, Tarla Bitkileri Merkez Arastirma Enstitusunde (TARM) gelistirilen mercimek hatlarinin IMI grubu herbisitlere karsi dayaniminin cesitliligini belirlemektir. Deneme 2014 yili sera, 2014/15 yilinda ise tarla kosullarinda 2 yil sure ile yurutulmustur. Denemede Tarla Bitkileri Merkez Arastirma Enstitusu Baklagil Islah Biriminde bulunan 759 adet hat materyal olarak kullanilmistir. 2014 yilinda serada yetistirilen genotiplere erken gelisme doneminde etken maddesi 40 g/L olan imazomax SL olan kimyasal kullanilmistir. Yapilan gozlem ve degerlendirmeler sonucu 36 kislik materyal secilmistir. Secilen kislik materyal 2014/2015 yetistirme doneminde bolunmus parsel deneme deseninde 3 tekrarlamali ve kontrol, normal ve 3 kati doz olacak sekilde Haymana’da tarlaya ekilmistir. Calisma sonucunda 35 numarali kislik kirmizi mercimek genotipinin IMI grubu herbisite tolerant oldugu belirlenmistir.