Zdzisława Zimniak-Przybylska

Diversity of seed globulins in Lathyrus sativus L. and some related species.

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of reduced seed globulins covered 141 accessions of Lathyrus sativus and the following related species: L. amphicarpos, L. blepharicarpus, L. cicera, L. gorgoni, L. marmoratus, L. pseudocicera and L. stenophyllus. In the total examined material 71 globulin polypeptide bands were distinguished. The number of polypeptide bands detected in particular species varied from 14 in L. amphicarpos to 29 in L. sativus; all the species under study showed intraspecific variation due to individual variation and/or differences among accessions. Electrophoretic data were subjected to statistical analysis using hierarchical UPGMA grouping. Frequency distribution of polypeptide bands in L. sativus showed some correlations with geographical origin and certain seed characteristics (seed coat colour, seed weight) of the studied accessions. As regards interspecific relationships, the studied species showed to be distantly related taxa except for the rather closely allied L. cicera and L. marmoratus. The obtained results are compared with the electrophoretic seed albumin data reported earlier for the same taxa.

Electrophoretic patterns of seed globulins in the Old-WorldLupinus species

SummaryElectrophoretic analysis of seed globulins was performed to investigate relationships between the Old-WorldLupinus species. The analysis covered 11 species described earlier and the recently reported taxon,Lupinus anatolicus nom. nud. With only two exceptions, particular species were represented by various accessions. In total, 110 accessions were examined. SDS-PAGE of reduced globulins was carried out in a discontinuous buffer system.The results showed a marked difference between the smooth-seeded and the rough-seeded species. In the group of smooth-seeded lupins pronounced interspecific differences were recorded. The rough-seeded species formed a rather homogenous group, well distinguishable from the smooth-seeded species. Though no distinct interspecific differences were recorded in the rough-seeded group, most of the 34 investigatedL. atlanticus accessions could be distinguished due to a characteristic, prominent polypeptide band with approx. Mr 27 kDa. The recently reported smooth-seeded taxon,L. anatolicus, showed a species-specific SDS-PAGE globulin pattern.

Electrophoretic seed albumin patterns and species relationships inVicia sect.Faba (Fabaceae)

Electrophoretic analysis of seed albumins (PAGE) covered 173 accessions representing nine species ofVicia sect.Faba. The number of albumin bands recorded in particular species varied from three inV. eristaloides to 23 inV. faba; in total, 38 bands were distinguished in the investigated material. The examined species, exceptV. eristalioides, showed intraspecific variation with respect to the number and relative staining intensity of albumin bands; individual variation was especially marked inV. faba and inV. narbonensis. Hierarchical clustering of the investigated taxa was based onBhattacharyya distances calculated from the electrophoretic data. The taxa grouped in three main clusters.Vicia faba and the rather remotely relatedV. kalakhensis formed one cluster. The second cluster was composed ofV. narbonensis distantly related toV. hyaeniscyamus. The third cluster comprised three subgroups: 1.V. johannis, V. galilaea andV. serratifolia, 2.V. eristalioides, and 3.V. bithynica. The obtained results are discussed with reference to taxonomic relationships inVicia sect.Faba.

Diversity of seed albumins in some Lathyrus species related to L. sativus L.: An electrophoretic study

Electrophoretic analysis of seed albumins covered 66 Lathyrus accessions representing L. amphicarpos, L. blepharicarpus, L. cicera, L. gorgoni, L. marmoratus, L. pseudocicera and L. stenophyllus. The number of major albumin bands recorded in particular species varied from two in L. amphicarpos to eight in L. gorgoni. Apart from L. amphicarpos, all the species under study showed intraspecific variation. In total, 21 bands were distinguished. The results were compared with the corresponding data reported earlier for L. sativus. All the data were statistically analysed. The species examined in this study proved to be distantly related to L. sativus. Distinctness of particular Lathyrus species was quite marked except for L. cicera and L. marmoratus which produced similar electrophoretic seed albumin patterns.

Diversity of seed albumins in the grasspea (Lathyrus sativus L.): an electrophoretic study

Electrophoretic analysis of seed albumins covered 105 Lathyrus sativus accessions originating from different geographical regions. Four major albumin bands were responsible for the detected variation. Due to differences in the number and combination of these bands, eleven electrophoretic seed albumin patterns were distinguished; four of them were rare phenotypes found in less than 1% of the 817 examined individuals. Electrophoretic data were subjected to statistical analysis using hierarchical UPGMA grouping and biplot techniques. Generally, no correlation was found between occurrence of either the seed albumin patterns or the major albumin bands and geographical origin of the forms under study. On the other hand, data concerning frequency distribution of the major albumin bands tended to separate two groups of accessions, white-seeded with large seeds and coloured-seeded with relatively small seeds; coloured-seeded accessions with rather large seeds mixed with the above groups.

Electrophoretic seed albumin patterns inVicia species of sectt.Hypechusa andPeregrinae (Fabaceae)

This work is a continuation of electrophoretic investigations aimed at revealing a wild relative ofVicia faba. Electrophoretic analysis (PAGE) of seed albumins covered 52 accessions representing eightVicia species of sect.Hypechusa and two species of sect.Peregrinae. Most of the examined species showed an intraspecific variation due to differences between accessions and/or individual variation within accessions. In spite of the intraspecific variation, marked interspecific differences were recorded. However, none of the investigated species displayed electrophoretic seed albumin patterns similar to those reported earlier forV. faba. Contribution of the obtained results to characterization of the examined taxa is discussed.

Isoenzyme variation in the wild relatives ofVicia faba (Fabaceae)

Electrophoretic analysis of five enzyme systems, LAP, PGI, SKDH, SOD and 6-PGDH, among 102Vicia accessions representingV. bithynica and seven species of theV. narbonensis complex, namelyV. eristalioides, V. kalakhensis, V. johannis, V. galilaea, V. serratifolia, V. narbonensis andV. hyaeniscyamus, has been performed. The recorded variation was tentatively assigned to 41 allelic genes at eight loci; intraspecific variation was observed in all species except forV. eristalioides. The results obtained were compared with the corresponding data reported earlier forV. faba. Hierarchical grouping of the investigated taxa, includingV. faba, was based onNeis genetic identities calculated from the allozyme frequency data.Vicia faba andV. bithynica were shown to be most distantly related to one another and to the remaining species investigated.Vicia serratifolia appeared to be a peripheral member of theV. narbonensis complex. The results are discussed with reference to genetic diversity and taxonomic relationships of the species under study.

Electrophoretic seed globulin patterns in some New World Lupinus species

Comparative SDS-PAGE analysis of seed globulins covered 74 accessions representing 19 New World Lupinus species, 18 North American lupins and one South American species: L. mutabilis. The species investigated showed major, well-defined polypeptide bands in the approximate Mr range of 40–75 kDa. In general, intraspecific variation blurred interspecific differences; L. mutabilis could not be distinguished from the North American lupins. Of the taxa investigated, only L. subcarnosus and L. succulentus displayed some species-specific features. The American species examined showed different seed globulin patterns from those reported earlier for the smooth-seeded and for the rough-seeded Old World lupins.

Structural characteristics of a major seed albumin ofPisum sativum

The major pea seed albumin fromPisum sativurn was carboxymethylated, cleaved with CNBr, and submitted to sequence analysis of the fragments in order to characterize the structural organization of the protein chains. Four major pools of largely homogeneous CNBr fragments were obtainede and likely N-and C-terminal fragments were identified. StruCtural analysis suggested the presence of single positions with microheterogeneities. It also revealed structures with long segments of distinct homology (52% structural identity), indicating the presence of different but related protein chains, or less likely, of repetitive structural elements within a chain. However, preparations appear largely homogeneous in protein class, and contain similar polypeptide chains of about 200 residues in mainly hydrophilic structures, with few methionine and cysteine/half-cystine residues.