Zekeriya Öztemur

Prevalence of known mutations in the MEFV gene in a population screening with high rate of carriers

The Familial Mediterranean Fever (FMF) shows an autosomal recessive pattern of inheritance and affects certain ethnic groups. Disease is caused by mutations in MEFV gene and more than 180 mutations have been defined in affected individuals. Current study aimed to determine the frequency-type of the mutations for MEFV gene in Sivas—middle Anatolian city. The cohort was composed of 3340 patients. MEFV gene mutations were studied by multiplex PCR based reverse hybridization stripAssay method. Patients’ clinical features were; family history: 68%, erysipelas-like erythema: 17.6%, fever: 89.9%, abdominal pain: 84.2%, peritonitis: 90.2%, arthritis: 33%, pleuritis: 14.2%, parental consanguinity: 21.2%. Current results revealed that M694V is the most frequent mutation (43.12%), followed by E148Q (20.18), M680I(G/C) (15.00%) and V726A (11.32%). The study population has a high rate of carriers and the E148Q mutation frequency was found to be highest when compared to the other regions of Turkey and other Mediterranean groups.

Long segment instrumentation of thoracolumbar burst fracture: fusion versus nonfusion.

OBJECTIVE The treatment of thoracolumbar burst fracture is a controversial issue. Although spinal fusion has been a touchstone of spinal fixation, nonfusion technique have become raising its popularity recently. Some studies suggested that nonfusion had several advantages over fusion. The aim of this prospective study was to compare long segment posterior instrumentation with fusion versus long-segment posterior instrumentation without fusion. METHODS For this purpose, 42 consecutive patients were assigned to two groups. Group 1 included 21 patients treated by long segment instrumentation with fusion (WF), whereas Group 2 included 21 patients treated by long segment instrumentation without fusion (WOF). Long segment instrumentation was hook fixation (claw hooks attached to second upper vertebra and infralaminar hooks attached to first upper vertebra) above and pedicle fixation (pedicle screws attached to first and second lower vertebrae) below the fractured vertebra. RESULTS Measurements of local kyphosis, sagittal index and anterior vertebral height compression showed that both group had similar outcome at final follow-up. Moreover, there was no difference between the two groups according to low back outcome score. Also, implant failure rate (4.7%) was quite low in both groups. However, WF group had prolonged operative time, increased blood loss and donor site morbidity. CONCLUSIONS Radiological and clinical parameters demonstrated that spinal fusion is not necessary in long segment posterior instrumentation for the management of thoracolumbar burst fractures.

Extreme complications of Fixion nail in treatment of long bone fractures.

The authors present their experience related to extreme complications in treatment of diaphyseal fractures of the long bones with application of the Fixion expansion intramedullary nail in a total of 48 surgeries. We have encountered six (12.5%) extreme complications in the management of fractures of 3 humeral, 2 tibial, and 1 femoral bones during its application procedure and postoperative follow-up. Of six cases, two with humeral and tibial fractures developed nonunion and rotational instability because of failure of inflation of the Fixion nail. One of the Fixion nail in humerus was broken spontaneously, and one of the Fixion nail deflated at the follow-up and pseudoarthrosis developed in this patient. In a patient with osteogenesis imperfecta, during the inflation of the nail for the treatment of femur fracture, a new longitudinal fracture occurred and conventional non-locking intramedullary nail was inserted. In a patient with a tibia fracture that was treated with the Fixion nail, new fracture occurred due to its bending after weight bearing in the postoperative period. The Fixion nail application is a new technique for the intramedullar fixation of long bones. It is considered as an effective method for the selective fracture types of long bones. Application may need special training. Since the Fixion has not got rotational stability and rigidity as conventional nailing systems, bending and breaking of the nail may occur during postoperative period in patients with over obesity and hyperactivity. In patients with osteogenesis imperfecta, it may not be the first choice as a nailing system.

School screening for scoliosis in Sivas, Turkey

OBJECTIVES We investigated the prevalence of scoliosis among primary school students in Sivas, Turkey. METHODS To determine the prevalence of scoliosis among primary school students in the age bracket of 10 to 15 years, 11 primary schools were selected with systematic sampling. A total of 3,175 students (1,538 girls, 1,637 boys) of grades 6 to 8 were selected from 16,103 students using a stratified sampling method. Examination for scoliosis was made by the forward bend test and palpation of the spine. RESULTS Fifteen children (0.47%) were found to have scoliosis, including 10 girls (66.7%) and five boys (33.3%). The prevalence of scoliosis was significantly higher in girls (0.65% vs. 0.31%; p<0.05). The mean lateral curvature of the spine was 6.9 degrees (range 5 degrees to 20 degrees ), being 7.9 + or - 4.6 degrees in girls, and 5.4 + or - 0.9 degrees in boys. The mean age was 13.5 + or - 1.2 years (range 12-15 years). The severity of the curvature showed no significant relationship with gender and age groups (p>0.05). The levels of involvement were lumbar vertebrae in 73.3% (n=11), thoracic vertebrae in 13.3% (n=2), and thoracolumbar vertebrae in 13.3% (n=2). Spinal curvature was to the right side in 12 cases (80%), and to the left in three cases (20%). Girls and boys did not differ significantly with respect to the severity and direction of the curvature (p>0.05). During a two-year follow-up of children with scoliosis, no progression of the curvature was observed, including one child who wore a Milwaukee brace. CONCLUSION School screenings may be performed as part of prevalence studies; however, routine school screening for scoliosis is debatable.

Anterior versus modified combined instrumentation for burst fractures of the thoracolumbar spine: a biomechanical study in calves

Purpose. To compare stability after anterior instrumentation alone versus modified combined anterior and posterior instrumentation for burst fractures of the thoracolumbar spine in calves. Methods. Thoracolumbar spines of 10 calves were used. An axial compression force was applied on each specimen using a material-testing machine, until there was a burst fracture at T12 or L1. Five specimens were fixed with anterior instrumentation alone, using 2 rods connected by 2 screws above and 2 screws below the fractured vertebra plus one tranverse connector. Another 5 were fixed with our modified technique of combined anterior and posterior instrumentation. This entailed one rod connected with one screw above and one screw below the fractured vertebra anteriorly, and another rod connected with one transpedicular screw above and one transpedicular screw below the fractured vertebra posteriorly. After instrumentation, the experiment was conducted again on each specimen and the compressive stiffness and vertebral height loss between the 2 groups compared. Results. The mean compressive stiffness was significantly greater after modified combined anterior and posterior instrumentation than anterior instrumentation alone (5508 vs 2888 N, p=0.0256), whereas the respective vertebral height losses were 37 and 33 mm (p=0.3808). Conclusion. Our modified technique of combined anterior and posterior instrumentation provides greater stability than traditional anterior instrumentation alone.

Kinesio taping as a treatment method in the acute phase of ACL reconstruction: A double-blind, placebo-controlled study

Objective In this study, we aimed on investigating the effects of Kinesio taping (KT) in acute postoperative rehabilitation phase of anterior cruciate ligament (ACL) reconstruction. Methods Thirty male patients (mean age: 28.1 years) with ACL reconstruction were randomly assigned to two groups: (1) an experimental group to receive a KT treatment through the muscle and lymphatic correction techniques; or (2) a control group for sham KT. Both interventions were applied twice during a 10-day period from the fourth postoperative day. All patients received the same rehabilitation program for three months. The groups were compared according to range of motion (ROM), pain, swelling and muscle strength before treatment and on the fifth and tenth treatment days. Subjective evaluations were made with the Lysholm, modified Cincinnati and Tegner scores on the first and third postoperative months. Results Intragroup comparisons showed significant improvements in both groups on the fifth and tenth day and first and third month evaluations (p < 0.05). In comparison to the control group, the experimental group showed significant improvements in swelling around the patella, all pain measurements and hamstring muscle strength on the fifth KT day and knee flexion range of motion (ROM), night pain, all swelling measurements and hamstring muscle strength on the tenth KT day (p < 0.05). Conclusion Our results revealed that KT techniques applied in addition to the acute rehabilitation program of ACL reconstruction are beneficial in treatment of pain, swelling, knee flexion ROM, and hamstring muscle strength. Level of evidence Level I, Therapeutic study.

Biomechanical properties of ciprofloxacin loaded bone cement

OBJECTIVE The purpose of this study was to investigate the biomechanical properties of bone cement used in joint replacement surgery after the addition of ciprofloxacin. METHODS The first group received bone cement only and served as a control for the 4 groups where 500 mg, 1000 mg, 1500 mg and 2000 mg of ciprofloxacin were added to yield 40 g of bone cement. Axial compression tests were conducted using a 50,000 Newton capacity tension-compression testing device. RESULTS While axial compression strength at failure was 80.2±4.3 MPa in the control group, values in the ciprofloxacin-treated groups decreased with rising concentration of ciprofloxacin to 74.5±5.4 MPa, 70.6±4.8 MPa, 70.5±4.7 MPa, and 69.3±3.4 MPa. CONCLUSION Bone cement with addition of 500 to 1500 mg ciprofloxacin maintained mechanical axial strength values above 70.0 MPa recommended by American Society for Testing and Materials and can be safely used in joint replacement surgery.

The effect of low dose teicoplanin-loaded acrylic bone cement on biocompatibility of bone cement.

Antibiotic-loaded acrylic bone cement (polymethylmethacrylate, PMMA) is used to prevent or treat infection in total joint replacement surgery. The purpose of this study was to investigate biocompatibility and cytotoxicity of the teicoplanin-loaded acrylic bone cement. Cytotoxicity examination of acrylic bone cement balls and 400 mg teicoplanin added acrylic bone cement balls conducted by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. SEM (Scanning electron microscopy) was used to observe adhesion and spreading of cells on surface of the balls. Cytotoxicity examination conducted by MTT assay on acrylic bone cement balls and teicoplanin-added acrylic bone cement balls revealed no cytotoxicity. SEM analysis put forward that cells started to proliferate and adhere on surface of the samples in both groups as a result of 48-hour incubation and that the cell proliferation over acrylic bone cement and teicoplanin-added acrylic bone cement was similar. As a consequence, there was no cytotoxicity in acrylic bone cement and teicoplanin-added acrylic bone cement groups according to results of MTT assay. On the other hand, results of SEM showed that biocompatibility of both groups was similar. In conclusion, teicoplanin-loaded bone cement did not change biocompatibility of bone cement in studied dose.

Treatment of Bifocal Cyst Hydatid Involvement in Right Femur with Teicoplanin Added Bone Cement and Albendazole

Although bone involvement associated with cyst hydatid is rarely seen, it can cause unintended results such as high recurrence rate, infection, sepsis, or amputation of relevant extremity. Because of this reason, its treatment is difficult and disputed. In the case of bifocal bone cyst hydatid in right femur, along with albendazole treatment, result of resecting cyst surgically and its treatment with teicoplanin with added bone cement is given. In conclusion, since the offered treatment method both supports bone in terms of mechanical aspect and also can prevent secondary infection, the method is thought to be a good and safe treatment approach.

Investigation of mechanical strength of teicoplanin and ciprofloxacin impregnated bone cement on Day 1 and Day 15

OBJECTIVE The aim of this study was to compare the mechanical effects of different concentrations of teicoplanin and ciprofloxacin addition in bone cement. METHODS In an experimental design, 3 different doses of teicoplanin and ciprofloxacin (800, 1600 and 3200 mg) were added to bone cement. Mechanical tests using compression and four-point bending tests were performed on Day 1 and after antibiotic leaching in water at 37°C on Day 15. Specimens that contained no antibiotics served as controls. Mechanical strength for each antibiotic concentration on Day 1 and Day 15 were evaluated. RESULTS Both teicoplanin and ciprofloxacin significantly decreased the mean strength values in compression and four-point bending tests at Days 1 and 15 (p<0.05). While teicoplanin significantly decreased the mean strength values at high doses in both tests at Days 1 and 15 (p<0.05), ciprofloxacin did not significantly change these values. When the effects of two drugs compared, there were significant differences at the 3200 mg dose at Day 1 and at 1600 and 3200 mg doses at Day 15 in the compression testing and at 3200 mg at Day 15 in the four-point bending test. CONCLUSION Teicoplanin and ciprofloxacin addition may adversely affect the biomechanical strength of bone cement. Ciprofloxacin addition seems to have less of a negative effect on strength than teicoplanin.