Zeng Lihua

Study on ultrastructural changes of anterior pituitary in rats exposed to PEMW

The document is to observe the changes of ultrastructure of endocrine cell in anterior pituitary after being exposed to PEMW to explore the possible injury characteristics. 28 male Sparague-Dawley rats were divided into 4 groups (7 rats in each group), the control group was put in the boxes without irradiation. At 24 h after exposure to PEMW (E=100 kVmiddotm-1, 10000, 100000, 300000 pulses), the transmission electron microscope was used to observe the ultrastructural changes of anterior pituitary. The result showed that ultrastructures of anterior pituitary were obviously changed in irradiated groups at 10000 pulses, especially in prolactin cells and growth hormone cells, including evacuation of secretory granule, the mitochondrial vacuolization and distinct dilatation of endoplasmic reticulum. The injury gradually aggravate followed by pulses increase. The changes at 300000 pulses were the most severe. In conclusion, PEMW can lead injury to endocrine cells of anterior pituitary in rats, mitochondria and rough endoplasmic reticulum are the main injured organelles.

Atomic force microscope observation on CHO cell membrane after Electromagnetic pulses irradiation

Atomic force microscope (AFM) has been used to visualize the morphological change on the surface of CHO cell membrane before and after Electromagnetic pulses (EMP)irradiation. The results showed that there were different sizes and shapes of membrane perforate (the width is 0.66-0.39 um, the depth is 392.95 nm) after 400 kV/m 200 pulses EMP irradiation. The numbers of cell membrane perforates were decreased with EMP field intensity and the pulses numbers decreasing. It is suggested that EMP irradiation can cause the membrane perforate of CHO cell directly. The numbers of cell membrane perforates were increased with EMP field intensity and the pulses numbers increasing. EMP induced membrane perforate may play a very important role in EMP bio-effects.

Effects of electromagnetic pulse on the tight junction of mice blood-testis-barrier

AIM. To investigate the changes of the mice testes and the tight junction (Tj) relative molecular and to identify the molecular mechanisms of the mice blood-testis-barrier (BTB) permea- bility increasing after the mice were exposed to electromagnetic pulse (EMP). METHODS. Observe the changes vimentin of the mice testes by laser scanning confocal technique. RESULTS. After the 200 kVm-1 EMP (the accumulative pulse times was 200) exposure, the vimentin in sustentacular cells of testis became irregularly shortened and derangement, and the intensity of immuno- fluorescence also showed ununiformity compared with control. CONCLUSION. The increasing of mice BTB permeability perhaps caused by the changing of vimentin.

Effects of EMP on the Hematological Parameters and Bone Marrow Cells of Mice

To evaluate the possible effects of EMP on blood system, blood samples and bone marrow cells of male BALB/c mice were collected for hematology determinations , CFU-GM colony assay, [Ca2+]i assessment and cell cycle measurement. Our results indicate that EMP can affect some parameters of blood system

Effect of electromagnetic pulse exposure on the permeability of blood-testicle barrier in mice

This paper study the effect of electromagnetic pulse (EMP) exposure on the permeability of blood-testicle barrier (BTB) in mice. Adult male BALB/c mice were exposed to EMP at 200 kV/m for 200 pulses with 2 seconds interval. The mice were injected with 2% Evans Blue solution through caudal vein at different time point after exposure, and the permeability of BTB was observed by fluorescence microscope. The testis sample for the transmission electron microscopy was prepared at 2h after EMP exposure. The permeability of BTB in mice was observed by using Evans Blue tracer and lanthanum nitrate tracer. After exposure, cloudy Evans Blue was found in the testicle convoluted seminiferous tubule of mice. Lanthanum nitrate was observed not only between testicle spermatogonia near seminiferous tubule wall and sertoli cells, but also between sertoli cells and primary spermatocyte or secondary spermatocyte. However, in control group lanthanum, nitrate was only found in the testicle sertoli cells between seminiferous tubule and near seminiferous tubule wall. EMP exposure could increase the permeability of BTB in the mice

Effects of DiLong oblatum on free radical oxidative injury after EMP radiation

The preventive and therapeutic effects of DiLong oblatum are observed on free radical oxidative injury in mouse brain and testis after EMP radiation. Activity of SOD was detected by NBT method and content of MDA was detected by TBA method. Compared with control group, activity of SOD in brain and testis decreased significantly and content of MDA in brain and testis increased significantly after EMP radiation. The activity of SOD in brain and testis increased significantly and content of MDA in brain and testis decreased significantly if DiLong oblatum was administered to mouse before EMP radiation. The activity of SOD in brain increased significantly and content of MDA in brain decreased significantly if DiLong oblatum was administered to mouse after EMP radiation. DiLong oblatum had some preventive and therapeutic effects on free radical oxidative injury in mouse brain after EMP radiation. DiLong oblatum had some preventive effects on free radical oxidative injury in mouse testis after EMP radiation

The gene expression of superoxide dismutase and the changes of the ultrastructure of rat testis after exposed to electromagnetics

The aim is to study the expression of superoxide dismutase (CuZnSOD, MnSOD) and the changes of ultrastructure in rat testis after electromagnetic radiation (EMR) so as to investigate the mechanisms of EMR effect on procreation. As an in vivo model, male SD rats were used. Rats were exposed to the field of electromagnetics. By reverse transcription-polymerase chain reaction (RT-PCR), manganese SOD (MnSOD) and copper/zinc SOD (CuZnSOD) mRNA in the testis have been tested and the testis samples were prepared for transmission electron microscopy to observe the ultrastructure at 6h, 12h, 24h and 48h after radiation. In rat testis, MnSOD mRNA expression decreased at 24h and CuZnSOD mRNA expression did not change after radiation; the ratio of MnSOD/CuZnSOD decreased at 24h after radiation. These were some degree injuries in the testis at different time after radiation, the testis damage at 24h was more serious than that at other times after radiation. At 24h after exposure, the chromatin was gathered in the border and the endoplasmic reticulum (ER) was dilated and vacuolated in interstitial cells, spermatogonium cells of rat testis. The changes in sustentacular cells was more serious than the other cells. The results showed that the MnSOD gene expression was decreased and the ultrastructure of rat testis were damaged after exposure to electromagnetics. These changes may induce the other effects, and the change of MnSOD expression might reflect a defensive mechanism of rat body against oxidative stress. If these changes would not regain in time, they may cause worse effects. Thus, EMR could induce some changes in the testis of rats so as to alter the procreative function.