Zha-Jun Zhan

α -Glucosidase Inhibitors from the Fungus Aspergillus terreus 3.05358

One new diketopiperazine alkaloid amauromine B (1), along with three known meroterpenoids, austalide B (2), austalides N and O (3 and 4), and two known steroids (5 and 6), was isolated and identified from the culture broth of the fungus Aspergillus terreus 3.05358. Their structures were elucidated by extensive spectroscopic techniques, including 2D‐NMR and MS analysis, the absolute configuration of 1 was unambiguously established by single crystal X‐ray diffraction analysis. All the isolates were evaluated for their inhibitory effects on α‐glucosidase. Amauromine B (1) and austalide N (3) exhibited more potent α‐glucosidase inhibitory activities than the positive control acarbose.

Two new tremulane sesquiterpenoids from Ceriporia lacerate , an endophytic fungus of Huperzia serrata

Two new tremulane sesquiterpenoids, ceriponols L–M, were isolated from the rice cultures of an endophytic fungus Ceriporia lacerate, derived from the stems of Huperzia serrata. The structures of the ceriponols L–M was established by spectroscopic methods, especially two-dimensional NMR techniques.

A new prenylated stilbene derivative from the roots of Cudrania tricuspidata

A new prenylated stilbene, 2-dimethylallyl-3,5,2’,4′-tetrahydroxy-(E)-stilbene, cudrastilbene, and five known compounds, oxyresveratrol, naringenin, steppogenin, aromadendrin, and genistein were isolated from the roots of Cudrania tricuspidata. The structure of the new compound was established by spectroscopic methods. All the compounds were evaluated for their cytotoxicity against three human tumour cell lines (HepG2, HCT-116, and SGC-7901). Cudrastilbene showed weak cytotoxic activity against the HepG2 cell line with an IC50 of 40.19 μM.

A novel ent-kaurane diterpenoid from Pteris semipinnata

The chemical investigation of the aerial parts of Pteris semipinnata furnished a novel ent-kaurane diterpenoid, and three known sesquiterpenoids with a 1-indanone skeleton, namely (2R)-pterosin B, (2S, 3S)-pterosin C and norpterosin C. The structure of 7β-hydroxy-11β, 16β-epoxy-ent-kauran-19-oic acid was established by spectroscopic methods, especially two dimensional NMR techniques.

Moniline, a new alkaloid from Dendrobium moniliforme

A new alkaloid, moniline (1), and a known alkaloid 6-hydroxynobiline (2) were isolated from the stems and leaves of Dendrobium moniliforme. The structure of 1 was established by spectroscopic methods, especially 2D NMR techniques.

Indole-benzodiazepine-2,5-dione derivatives from Neosartorya fischeri

One new aszonalenin analogue, 6-hydroxyaszonalenin was isolated from the culture of Neosartorya fischeri CGMCC 3.5378, together with aszonalenin, acetylaszonalenin, fumitremorgin B, verruculogen, and aszonapyrones A and B. The structure of the new metabolite was elucidated by comprehensive spectroscopic analyses, especially HR-ESI-MS and NMR experiments. The absolute configuration of 6-hydroxyaszonalenin was determined by X-ray diffraction. To the best of our knowledge, aszonapyrone B was reported for the first time from this species.

New polyhydroxypregnane glycosides from Cynanchum otophyllum

Two new polyhydroxypregnane glycosides, namely cynotophyllosides G-H (1-2), were isolated from the roots of Cynanchum otophyllum, together with three known steroids. Their structures were established by spectroscopic methods and acid-catalysed hydrolysis. Cynanchum otophyllum also known as “Qingyangshen“ in traditional Chinese medicine, is a perennial weed which has been used locally to treat epilepsy, rheumatic pain, kidney weakness, and muscle injuries.

A new alkaloid from the mycelium of Inonotus obliquus

A new alkaloid, inotopyrrole (N-phenethyl-2-formyl-5-hydroxymethylpyrrole), was isolated from the mycelium of Inonotus obliquus, together with three known compounds. The structure of inotopyrrole was established by detailed analysis of its spectroscopic data, especially the one- and two-dimensional NMR spectra and HR-ESI-MS data.

Ervahainanmine, a new indole alkaloid from the stems of Ervatamia hainanensis

A new sarpagine-type alkaloid with N-oxide group, called ervahainanmine, and three known ones including coronaridine, 19(S)-heyneanineguan, and coronaridine hydroxyindolenineguan were isolated from the stems of Evratamia hainanensis. The structure was established by spectroscopic methods, especially 2D NMR techniques.

Ceriponol P, the first example of monocyclic tremulane sesquiterpene produced by Ceriporia lacerate a fungal endophyte of Huperzia serrata

A new tremulane sesquiterpene, ceriponol P, has been isolated from the cultures of Ceriporia lacerate, together with three known metabolites 3-indolecarbaldehyde, 3-methyl-2-indolinone, and daucosterol. Its structure was established by detailed analysis of its spectroscopic data, especially 1D, 2D NMR and HR-ESI-MS data. Ceriponol P represents the first example of monocyclic tremulane sesquiterpene. In biological assays, ceriponol P did not exhibit any significant cytotoxicity against HeLa, HepG2, and SGC 7901 cell lines at a concentration of 20 μM. All the known compounds were reported for the first time from the genus Ceriporia.