Zhai Suoqiang

Isolation and Induction of Differentiation of Seine Adipose-Derived Mesenchymal Stem Cells

Abstract Objectives To establish a method for high yield mesenchymal stem cells collection, as well as a culture method for identifying mesenchymal stem cells from the swine adipose-derived mesenchymal stem cell (ADMSC). Methods Swine ADMSCs were isolated from fat tissue with collagenase, followed by induction of differentiation to osteogenic, adipogenic and chondrogrnic cells. The survival curve of the ADMSC at the 37°C and 38°C were measured using WST-1Cell Proliferation Assay Reagent. Result ADMSCs isolated with collagenase from swine neck fat tissue generated a stable uniform appearance after the second generation. The passage period was five days. ADMSC could differentiate into osteogenic, adipogenic or chondrogrnic cells under different culture conditions. The highest growth rate was achieved at 38°C in this study. Conclusion Swine ADMSCs have the potential to differentiate into osteogenic, adipogenic or chondrogrnic cells, and they may be appropriate for transplantation for both research and clinical purpose.

Auditory characteristics of noise-exposed memberscrossing age-related groups

Objective To report audiological characteristics in a group of noise-exposed crew members on board ships. Methods and materials Clinical and audiological measurements including pure-tone thresholds, acoustic immittance results and tinnitus questionnaires were collected from both the ship crew members (study subjects) and their land based colleagues (controls). Results 1) Noise exposed crew members showed not only high frequency, but also low frequency hearing loss; 2) Hearing impairment increased with age, with 65.5% of crew members younger than 50 years showing normal hearing while only 14.9% of those older than 50 years had normal hearing; 3) hearing loss gradually increased with the extension of on board career time; and 4) Most study subjects reported high pitch tinnitus, significantly more than the control group although not significantly different among different age groups. Conclusion Noise induced hearing impairment from working on board ships shows specific frequency and age characteristics. Understanding these characteristics is important for advancing relevant studies and for effective prevention of noise-induced hearing loss in ship crew members.