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Dive into the research topics where Zhang Jinyong is active.

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Featured researches published by Zhang Jinyong.


Molecular Biology Reports | 2011

Fusion expression and immunogenicity of Bordetella pertussis PTS1-FHA protein: implications for the vaccine development

Zhang Jinyong; Zhang Xiaoli; Zhang Weijun; Guo Ying; Guo Gang; Mao Xuhu; Zou Quanming

Mutants of pertussis toxin (PT) S1 subunit and filamentous hemagglutinin (FHA) type I immunodominant domain from Bordetella pertussis (B. pertussis) are considered to be effective candidate antigens for acellular pertussis vaccines; however, the substantial progress is hampered in part for the lack of a suitable in vitro expression system. In this paper, the gene sequences of a S1 mutant C180-R9K/E129G (mS1) and a truncated peptide named Fs from FHA type I immunodominant domain were linked together and constructed to pET22b expression vector as a fusion gene; after inducing with IPTG, it was highly expressed in E. coli BL21 (DE3) as inclusion body. The fusion protein FsmS1 was purified from cell lysates and refolded successfully. The result of Western blotting indicate that it was able to react with both anti-S1 and anti-FHA McAbs; antiserum produced from New Zealand white rabbits immunized with this protein was able to recognize both native PT and FHA antigens as determined by western blotting. These data have provided a novel feasible method to produce PT S1 subunit and FHA type I immunodominant domain in large scale in vitro, which is implicated for the development of multivalent subunit vaccines candidate against B. pertussis infection.


Archive | 2013

Purification method of methicillin-resistant staphylococcus aureus (MRSA) vaccine recombinant protein antigen FnbA1

Zhang Jinyong; Zou Quanming; Guo Ying; Feng Qiang; Fan Shaowen; Lu Lu; Dong Yandong; Jing Haiming; Gu Jiang


Archive | 2014

Methicillin-resistant staphylococcus aureus (MRSA) vaccine recombinant protein antigen HI2 and preparation method and application thereof

Zou Quanming; Fan Shaowen; Lu Lu; Zeng Hao; Feng Qiang; Zuo Qianfei; Zhang Jinyong; Gu Jiang; Lu Dongshui


Archive | 2014

Helicobacter pylori antigen HLA restrictive immunodominance epitope peptide and preparation method and application thereof

Wu Chao; Zou Quanming; Guo Hong; Yang Wuchen; Chen Li; Li Bin; Zhao Zhuo; Zhang Jinyong; Li Haibo; Mao Xuhu; Guo Gang; Tong Wende; Lu Dongshui


Archive | 2012

Helicobacter pylori multiple-epitope fusion protein and multiple-epitope vaccine prepared by helicobacter pylori multiple-epitope fusion protein

Wu Chao; Li Haibo; Zou Quanming; Zhang Jinyong; Liu Kaiyun; Yang Wuchen; Chen Li; Li Bin; Zhao Zhuo; Mao Xuhu; Guo Gang; Tong Wende; Lu Dongshui


Archive | 2017

Helicobacter pylori dominant antigen assembly based on CD4+T cell immunity and screening method

Wu Chao; Zou Quanming; Sun Heqiang; Yuan Hanmei; Zhao Zhuo; Tan Ranjing; Li Bin; Guo Gang; Zhang Jinyong; Jing Haiming; Qin Yi


Archive | 2017

Helicobacter pylori multi-subunit vaccine based on CD4+T cellular immunity and preparing method

Wu Chao; Zou Quanming; Sun Heqiang; Yuan Hanmei; Zhao Zhuo; Tan Ranjing; Li Bin; Guo Gang; Zhang Jinyong; Jing Haiming; Qin Yi


Archive | 2016

Pseudomonas aeruginosa recombinant protein Vac14 as well as preparation method and application

Gu Jiang; Zou Quanming; Zhang Jinyong; Yang Feng; Yang Xi; Zhao Liqun; Peng Liusheng; Wu Yi; Wang Yilin


Archive | 2016

PA (pseudomonas aeruginosa) recombinant protein Vac33, as well as preparation method and application thereof

Gu Jiang; Zhang Jinyong; Zou Quanming; Zhao Liqun; Yang Xi; Yang Feng; Peng Liusheng; Wu Yi; Wang Yilin


Archive | 2016

Building method for mouse model systemically infected with pseudomonas aeruginosa

Zhang Jinyong; Gu Jiang; Yang Feng; Zou Quanming; Peng Liusheng; Zhao Liqun; Jing Haiming; Wang Yilin; Qin Yi

Collaboration


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Zou Quanming

Third Military Medical University

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Jing Haiming

Third Military Medical University

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Zeng Hao

Third Military Medical University

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Wu Yi

Third Military Medical University

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Dong Yandong

Third Military Medical University

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Guo Gang

Third Military Medical University

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Lu Lu

Third Military Medical University

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Fan Shaowen

Third Military Medical University

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Cai Changzhi

Third Military Medical University

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