Zhang Jinyong
Third Military Medical University
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Publication
Featured researches published by Zhang Jinyong.
Molecular Biology Reports | 2011
Zhang Jinyong; Zhang Xiaoli; Zhang Weijun; Guo Ying; Guo Gang; Mao Xuhu; Zou Quanming
Mutants of pertussis toxin (PT) S1 subunit and filamentous hemagglutinin (FHA) type I immunodominant domain from Bordetella pertussis (B. pertussis) are considered to be effective candidate antigens for acellular pertussis vaccines; however, the substantial progress is hampered in part for the lack of a suitable in vitro expression system. In this paper, the gene sequences of a S1 mutant C180-R9K/E129G (mS1) and a truncated peptide named Fs from FHA type I immunodominant domain were linked together and constructed to pET22b expression vector as a fusion gene; after inducing with IPTG, it was highly expressed in E. coli BL21 (DE3) as inclusion body. The fusion protein FsmS1 was purified from cell lysates and refolded successfully. The result of Western blotting indicate that it was able to react with both anti-S1 and anti-FHA McAbs; antiserum produced from New Zealand white rabbits immunized with this protein was able to recognize both native PT and FHA antigens as determined by western blotting. These data have provided a novel feasible method to produce PT S1 subunit and FHA type I immunodominant domain in large scale in vitro, which is implicated for the development of multivalent subunit vaccines candidate against B. pertussis infection.
Archive | 2013
Zhang Jinyong; Zou Quanming; Guo Ying; Feng Qiang; Fan Shaowen; Lu Lu; Dong Yandong; Jing Haiming; Gu Jiang
Archive | 2014
Zou Quanming; Fan Shaowen; Lu Lu; Zeng Hao; Feng Qiang; Zuo Qianfei; Zhang Jinyong; Gu Jiang; Lu Dongshui
Archive | 2014
Wu Chao; Zou Quanming; Guo Hong; Yang Wuchen; Chen Li; Li Bin; Zhao Zhuo; Zhang Jinyong; Li Haibo; Mao Xuhu; Guo Gang; Tong Wende; Lu Dongshui
Archive | 2012
Wu Chao; Li Haibo; Zou Quanming; Zhang Jinyong; Liu Kaiyun; Yang Wuchen; Chen Li; Li Bin; Zhao Zhuo; Mao Xuhu; Guo Gang; Tong Wende; Lu Dongshui
Archive | 2017
Wu Chao; Zou Quanming; Sun Heqiang; Yuan Hanmei; Zhao Zhuo; Tan Ranjing; Li Bin; Guo Gang; Zhang Jinyong; Jing Haiming; Qin Yi
Archive | 2017
Wu Chao; Zou Quanming; Sun Heqiang; Yuan Hanmei; Zhao Zhuo; Tan Ranjing; Li Bin; Guo Gang; Zhang Jinyong; Jing Haiming; Qin Yi
Archive | 2016
Gu Jiang; Zou Quanming; Zhang Jinyong; Yang Feng; Yang Xi; Zhao Liqun; Peng Liusheng; Wu Yi; Wang Yilin
Archive | 2016
Gu Jiang; Zhang Jinyong; Zou Quanming; Zhao Liqun; Yang Xi; Yang Feng; Peng Liusheng; Wu Yi; Wang Yilin
Archive | 2016
Zhang Jinyong; Gu Jiang; Yang Feng; Zou Quanming; Peng Liusheng; Zhao Liqun; Jing Haiming; Wang Yilin; Qin Yi