Zhang Yun-wei

Effects of space flight factors on genetic diversity of Buchloe dactyloides seeds

The objective of this research was to investigate the effects of space flight factors on Buchloe dactyloides “Jingyin No.3” seeds. After the retrieval, basic turf characters of plants were tested. Among the 100 plants tested, 21 showed great change on phenotype characters, including leaf blade length and width, height, stem diameter, number of tillers, number and length of stolon, length of stolon inter node, leaf color and extent of leaf turning yellow. 33 primers were screened in inter-simple sequence repeats (ISSR) analysis to evaluate DNA variation between mutations and their ground controls. Results show that 15.6 reliable bands were generated by 7 primers, of which 12.9 (80.9%) were polymorphic. Based on the study, we can conclude that the space flight factors could induce inheritable mutagenic changes on B. dactyloides seeds, and do further research to demonstrate these changes in genetic material of the mutants. Key words : Genetic diversity, Buchloe dactyloides, spaceflight, inter-simple sequence repeats.

Effects of additives on fermentation quality of alfalfa silage

The objective of this study is to determine the effects of acetic acid, propionic acid, lactic acid bacterial, cellulose and urea additives on the fermentation qualities of whole-plant alfalfa growth in the first year. From the results, all additives except for urea significantly improved the fermentation quality of alfalfa and decreased pH and ammoniacal nitrogen content. The interaction of additives and varieties significantly affected all testing items for fermentation quality (p<0.01). Additives had a significant effect on pH and lactic acid bacterial content of different alfalfa varieties (p<0.01) and also affected NH3-N/TN (p<0.05). However, additives showed no significant effect on content of acetic acid, propionic acid and butyric acid. Among all varieties, God Empress had a superior silage quality with a lowest pH and highest lactic acid content and a relatively low NH3-N/TN.