Zhao MingQin

Analysis of enzyme activity and chemical composition of different varieties of flue-cured tobacco after topping in Nanyang tobacco-growing areas

Using ten flue-cured tobacco cultivars, namely NK4, S2007, Yu5, Yu6, 8342, 8302, NC89, Honghuadajinyuan, Y041, Yun 87 and so on, as material, activity changes of nitrate reductase (NR), invertase (Inv), α-amylase(AM) and coordination of chemical constituents in different varieties of flued-cured tobacco when topping and 10, 20, 30, 40 d after topping were studied. The results showed that: 1) Activity of NR and Inv and accumulation of carbohydrates in tobacco plants of Yu5 and NK4 was higher in early maturity stage, followed by continued decreasing. AM activity of Yu6 and NK4 in late maturity stage was higher, making a slow reduction of carbohydrate metabolism intensity,which was conducive to fully mature leaf. 2) Amount of various chemical components of Yu5 and NK4 were in optimal range, with chemical composition in coordination, internal quality prominent. So NK4 and Yu5 planted in Nanyang were more suitable to be cultivated in Nangang tobacco-growing areas.

Structure Verification and Thermal Decomposition Kinetics of β-Ionone and β-Cyclodextrin Inclusion Compound

An inclusion compound of β-ionone and β-cyclodextrin was prepared from their saturated solution,analyzed by thermogravimetric analyzer,differential thermal analyzer and IR spectrometer,and applied to cigarette as a flavor.The results showed that: 1) The obtained compound was verified;2) The thermal stability of the inclusion compound was higher than β-ionone,the bonding of β-ionone and β-cyclodextrin was mainly the results of Van der Waals force;3) The inclusion compound enhanced the aroma quality and quantity,improved aftertaste and reduced irritation of cigarette smoke.

Effect of spraying apical regulator on chromoplast pigment and its degradation products in flue-cured tobacco under conditions of varied nitrogen rate.

Taking the flue-cured tobacco variety Jiyan No.9 as material,the combined effects of varied nitrogen volume and apical regulator on chromoplast pigment and the content of its degradation products in flue-cured tobacco leaf were determined.Results suggested that both of the treatments,namely different nitrogen volume and apical regulator,significantly affected chromoplast pigment and the content of its degradation products in post-cured tobacco leaf,additionally,the combined effects of the treatments mentioned above on carotenoid reached to extremely significant level.Findings were:1) The content of chlorophyll,carotenoid and their decomposition products in tobacco leaf of medium nitrogen rate treatments were relatively higher and could meet the demand for nitrogen element during growth and development of tobacco;2) Apical regulator probably enhanced the physiological activity of tobacco plant and promoted the degradation and conversion of chromoplast pigment.The content of neophytadiene and the degradation products of carotenoid in treatment of apical regulator B,particularly,evidently higher than that of other ones.Comparison of different treatments,as existing interactive action between two factors,the treatment of T8,namely medium nitrogen volume coupled with apical regulator B,could achieve the highest content of chromoplast pigment degradation products in post-cured tobacco and also relatively better tobacco character.