Zhengchen Zhang

Salvianolic acid B attenuates apoptosis and inflammation via SIRT1 activation in experimental stroke rats

Silent information regulator 1 (SIRT1), a histone deacetylase, has been suggested to be effective in ischemic brain diseases. Salvianolic acid B (SalB) is a polyphenolic and one of the active components of Salvia miltiorrhiza Bunge. Previous studies suggested that SalB is protective against ischemic stroke. However, the role of SIRT1 in the protective effect of SalB against cerebral ischemia has not been explored. In this study, the rat brain was subjected to middle cerebral artery occlusion (MCAO). Before this surgery, rats were intraperitoneally administrated SalB with or without EX527, a specific SIRT1 inhibitor. The infarct volume, neurological score and brain water content were assessed. In addition, levels of TNF-α and IL-1β in the brain tissues were detected by commercial ELISA kits. And the expression levels of SIRT, Ac-FOXO1, Bcl-2 and Bax were detected by Western blot. The results suggested that SalB exerted a cerebral-protective effect, as shown by reduced infarct volume, lowered brain edema and increased neurological scores. SalB also exerted anti-inflammatory effects as indicated by the decreased TNF-α and IL-1β levels in the brain tissue. Moreover, SalB upregulated the expression of SIRT1 and Bcl-2 and downregulated the expression of Ac-FOXO1 and Bax. These effects of SalB were abolished by EX527 treatment. In summary, our results demonstrate that SalB treatment attenuates brain injury induced by ischemic stoke via reducing apoptosis and inflammation through the activation of SIRT1 signaling.

Bone pain caused by swelling of mouse ear capsule static xylene and effects on rat models of cervical spondylosis

To observe the effect of intravenous bone pain Capsule on the ear of mice induced by xylene, swelling of rat models of cervical spondylosis. Weighing 18 ∼ 21g 50 mice, male, were randomly divided into for five groups, which were fed with service for bone pain static capsule suspension, Jingfukang granule suspension 0.5%CMC liquid and the same volume of. Respectively to the mice ear drop of xylene 0.05u2005ml, 4h after cervical dislocation, the mice were sacrificed and the cut two ear, rapid analytical balance weighing, and calculate the ear swelling degree and the other to take the weight of 200 - 60 250g male SD rats, were randomly divided into for 6 groups, 10 rats in each group, of which 5 groups made cervical spondylosis model. Results: with the blank group than bone pain static capsule group and Jingfukang granule group can significantly reduce mouse auricular dimethylbenzene swelling, significantly reduce ear swelling degree (P < 0.01); the successful establishment of the rat model of cervical spondylosis. With the model group ratio, large, medium and small dose of bone pain static capsule group, Jingfukang granule group (P < 0.01) angle of swash plate of rats increased significantly, the high and middle dose of bone pain static capsule group, Jingfukang granule group can significantly reduce the rat X-ray scores (P < 0.05). Bone pain static capsule can significantly reduce mouse auricular dimethylbenzene swelling. The bone pain capsule has a good effect on the rat model of cervical spondylosis.To observe the effect of intravenous bone pain Capsule on the ear of mice induced by xylene, swelling of rat models of cervical spondylosis. Weighing 18 ∼ 21g 50 mice, male, were randomly divided into for five groups, which were fed with service for bone pain static capsule suspension, Jingfukang granule suspension 0.5%CMC liquid and the same volume of. Respectively to the mice ear drop of xylene 0.05u2005ml, 4h after cervical dislocation, the mice were sacrificed and the cut two ear, rapid analytical balance weighing, and calculate the ear swelling degree and the other to take the weight of 200 - 60 250g male SD rats, were randomly divided into for 6 groups, 10 rats in each group, of which 5 groups made cervical spondylosis model. Results: with the blank group than bone pain static capsule group and Jingfukang granule group can significantly reduce mouse auricular dimethylbenzene swelling, significantly reduce ear swelling degree (P < 0.01); the successful establishment of the rat model of cervical spondylos...

Effect of Qingnao tablet on blood viscosity of rat model of blood stasis induced by epinephrine

To establish a rat model of blood stasis with adrenaline (Adr) subcutaneous injection and ice bath stimulation. The effects of different doses on the blood viscosity of blood stasis model rats were observed. The rats were randomly divided into 6 groups: blank control group (no model), model group, positive control group, high, middle and low dose group. The whole blood viscosity and plasma viscosity were detected by blood viscosity instrument. Compared with the blank group, model group, high shear, low shear whole blood viscosity and plasma viscosity were significantly increased, TT PT significantly shortened, APTT was significantly prolonged, FIB increased significantly, indicating that the model was successful. Compared with the model group, can significantly reduce the Naoluotong group (cut, low cut). Qingnaopian high dose group (low cut), middle dose group (cut, low shear blood viscosity) (P<0.01), Can significantly reduce Naoluotong qingnaopian group, high dose group (P<0.01), plasma viscosity decrea...

Protective role of Xinnaoning tablet in ischemic stroke in rat model

Background: Stroke has been considered as the second leading cause of death worldwide. The survivors of stroke experience different level of impair brain function. In China, Chinese traditional medicine had been widely accepted for stroke therapy and prevention. In this study, we developed Traditional Chinese Medicine based Xinnaoning (peace of heart and brain) Tablet and tested its protective role for ischemic stroke in rat model. nMaterial and Methods: Male Wistar rats (n=60) with 12 weeks old and weight from 180 to 200 gram were randomly divided to five groups (n=12). For the groups with Xinaoning administration, the drug was administrated to rats once per day for 7 consecutive days. The blood clotting time and the thrombus wet weight was measured. Serum samples were collected from each rat for further Measurement of biochemical indicators. nResults: Our results demonstrated that Xinnaoning tablet reduced lactate acid (LD) level and increased lactic acid dehydrogenase (LDH) in cerebral ischemia model as well as reduced the infarct size caused by stroke. Besides, evaluation of the level of different ATPases suggested Xinnaoning tablet could modulate ATPases activity and confer a protective role in brain. Moreover, analysis indicated Xinnaoning tablet have the anti-coagulation effect in vivo which may contribute to the protection of ischemia. nConclusion: Our findings suggest that Xinnaoning tablet may be a potential way for cerebral ischemia prevention.

Experimental study on skin irritation of bone spur powder on rabbit

To observe the effect of bone powder of rabbit skin, provide the basis for the safety of clinical use of bone powder, 24 rabbits were randomly divided into 6 groups, complete skin test and damaged skin test each divided into 3 groups (n=4), high, low, 3 doses tested daily administered 1 times, continuous administration for 7 days, in 24 hours after the last administration of drug residues, wash with warm water, the removal of L hours after drug for 24 hours, 48 hours, 72 hours and seventh days, observed and recorded to apply position before administration and administration during the skin no erythema and edema, and observe the smear Parts of any pigmentation, bleeding, rough skin or thin skin etc., record the occurrence time and duration time. Through comparative observation, intact skin group before administration and dosing period, there were no erythema and edema, pigmentation, bleeding, rough skin or thin skin etc., there is no difference with the control group; the damaged skin group after administr...

Study on acute toxicity of anti-vertigo granule on mice

To observe the effect of anti - glare particles on acute toxicity of mice. Methods: 40 male and female mice weighing 18 - 21u2005g were randomly divided into anti - glare granule group and normal saline control group. The maximum volume of anti - glare particles (0.94u2005g/ml) was administered before the experiment. Results: the oral toxicity of the suspension was very small. The maximal concentration of mice was given at the maximum volume of gastric perfusion, and it was given three times in 1st. The cumulative maximum tolerance dose was 112.8g/kg per day. The dose was 226 times of clinical dosage and no death was found in mice. Conclusion: the toxicity of Kangxuan granules is very small and it can be considered safe in clinical use.

Effect of Qing Nao tablet on blood stasis model of mice

To investigate the effect of Qing Nao tablet on mouse model of blood stasis syndrome, 60 mice, male and female, were randomly divided into 6 groups, were fed with large, small doses of Qing Nao tablet suspension, Naoluotong saline suspension and the same volume (group 2, 0.1ml/10g), administer 1 times daily, orally for 15 days. Intragastric administration for first days, in addition to the 1 group saline group every day in the hind leg intramuscular saline, the other 5 groups each rat day hind leg muscle injection of dexamethasone 0.8mg/kg intramuscular injection every day, 1 times, 15 days. 1 hour continuous intramuscular injection and intramuscular drug perfusion on the sixteenth day after mice. The eyeball blood, heparin after whole blood viscosity test. Compared with the control group, model group, high and low shear viscosity were significantly increased (P<0.01), indicating that the model was successful. Compared with the model group, high dose group and Qing Nao tablet Naoluotong group can signific...

Investigation of hemorheological and analgesic properties of Qing-Nao tablets

Purpose: To investigate the hemorheological and analgesic potentials of Qing-Nao tablet (QNT). Methods: Sixty animals were divided into 6 groups ( n = 10 for each group): normal, control, positive and 3 QNT-treated groups (QNT-H, QNT-M, QNT-L). Animals in normal and control groups received normal saline orally, while those in positive and QNT groups were orally administered either a suspension of the contents of Nao-luo-tong capsules or QNT. Hemorheological indices, including blood viscosity, plasma viscosity, activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT) and fibrinogen (FIB), were determined. Acetic acid-induced writhing, tail tenderness and hot plate tests, as well as tests on microcirculation and balance/coordination were also carried out. Results: Acute toxicity results showed that QNT is a safe drug. Whole blood and plasma viscosities of the 3 QNT-treated groups significantly decreased ( p < 0.05). However, TT levels of the 3 QNT-treated animals significantly increased ( p < 0.05), whereas APTT and FIB values decreased significantly ( p < 0.01). Moreover, QNT significantly increased analgesia in three animal models ( p < 0.05). QNT-H also significantly improved balance and coordination abilities of mice ( p < 0.05). Conclusion: These results demonstrate that QNT improves microcirculation and analgesia, and may be a source of promising candidate drugs for the treatment of brain concussion sequelae. Keywords: Qing-Nao tablet, Hemorheological, Analgesic effects, Brain concussion sequelae, Microcirculation, Balance and coordination abilities

Effect of lipid lowering tablet on blood lipid in hyperlipidemia model rats

Observe the effect of lipid-lowering tablets on body weight, liver index and serum biochemical indexes of hyperlipidemia rats. The hyperlipidemia rat model was replicated successfully. Compared with the model group, high, medium and low dose lipid-lowering tablets group could significantly increase the body weight of rats with hyperlipidemia (Pu202f<u202f0.01, Pu202f<u202f0.05); High and middle dose lipid-lowering tablets group could significantly reduce the liver index of high fat rat (Pu202f<u202f0.01); High, medium and low dose lipid-lowering tablets group could significantly decrease levels of TC, TG, LDL-C, AST, ALT, ALP, Y-GT in serum (Pu202f<u202f0.01, Pu202f<u202f0.05), and significantly increase the level of HDL-C (Pu202f<u202f0.01). Lipid-lowering tablets can effectively regulate the body lipid metabolism of rats, and have a certain therapeutic effect on hyperlipidemia.