Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Zhi-Hui Zhang is active.

Publication


Featured researches published by Zhi-Hui Zhang.


Journal of Medicinal Chemistry | 2015

Synthesis and Structure–Activity Relationships of Quaternary Coptisine Derivatives as Potential Anti-ulcerative Colitis Agents

Zhi-Hui Zhang; Hai-Jing Zhang; An-Jun Deng; Bo Wang; Zhi-Hong Li; Yang Liu; Lian-Qiu Wu; Wen-Jie Wang; Hai-Lin Qin

Thirty quaternary coptisine derivatives from a synthesized library were found to activate the in vitro transcription of x-box-binding protein 1 (XBP1). Among these, the dihydrocoptisines were demonstrated by in vitro XBP1 transcriptional activity assays and animal experiments to be much more active anti-ulcerative colitis (UC) agents than quaternary coptisines, tetrahydrocoptisines, and the positive control. Unsubstituted dihydrocoptisine exhibited more significant anti-UC efficacy than dihydrocoptisines substituted at the C-8 or C-13 position. The EC50 value of dihydrocoptisine for XBP1 transcriptional activation was 2.25 nM. Dihydrocoptisine exhibited a significant dose-effect relationship, as indicated by biomarkers in in vitro and in vivo experiments. According to this study, the starting materials reductive states and the substitution patterns of the dihydrocoptisines were determined to be the critical parameters for modulating their anti-UC efficacy, and the dihydrocoptisine skeleton was designated as the key pharmacophore. The synthesized dihydrocoptisine is a promising lead for developing anti-UC drugs.


Fitoterapia | 2013

Osteoclast-inhibiting saikosaponin derivatives from Bupleurum Chinense

Jin-Qian Yu; An-Jun Deng; Lian-Qiu Wu; Zhi-Hui Zhang; Yang Liu; Wen-Jie Wang; Hai-Lin Qin

Five new saikosaponins, saikosaponin w (1), 21β-hydroxysaikosaponin b2 (2), 6″-O-acetylsaikosaponin e (3), 6″-O-acetylsaikosaponin b1 (4), and 6″-O-acetylsaikosaponin b3 (5), along with twenty-two known ones (6-27), have been isolated from the roots of Bupleurum chinense. Their structures were elucidated on the basis of detailed spectroscopic analysis, including mainly 1D and 2D NMR and HRESI-MS, qualitative chemical methods, and comparison with the literatures. Osteoclast-inhibiting activity of some of the isolated compounds was evaluated in vitro, with five ones have shown significant activity by inhibitory rates ranging from 48.3% to 56.1% at the concentration of 10μM and with significant differences among groups observed.


Pharmacological Reports | 2016

The protective effect of epicatechin on experimental ulcerative colitis in mice is mediated by increasing antioxidation and by the inhibition of NF-κB pathway

Hai-Jing Zhang; An-Jun Deng; Zhi-Hui Zhang; ZiHan Yu; Yang Liu; Shan-Ying Peng; Lian-Qiu Wu; Hai-Lin Qin; Wen-Jie Wang

BACKGROUND Ulcerative colitis (UC) is a chronic inflammatory intestinal disease. It is necessary to find out new effective drugs for UC. In our study epicatechin extracted from grape seed by our institute for the first time could treat UC effectively. Then anti-UC mechanisms of epicatechin were elucidated in vivo and in vitro. METHODS Dextran sulfate sodium (DSS)-induced acute UC mice model was used to evaluate the activity of epicatechin and its properties against UC. Then its anti-inflammatory and antioxidant effects were evaluated as follows: the concentrations of TNF-α and IL-6 in the colon supernatants were determined by ELISA. NO and MPO were assayed by Griess method and commercial kit respectively. NF-κB were determined by NF-κB-Dependent Reporter Gene Expression Assay and Western Blotting respectively. Antioxidant factors such as SOD, MDA, GSH-Px and CAT were also measured in colon tissues and cell supernatant stimulated by LPS respectively. RESULTS In C57BL/6J mice model with DSS-induced UC, epicatechin was able to decrease the disease activity index and colon macroscopic damage index scores, reduce body weight loss, and significantly relieve colon contracture and crypt damage. TNF-α, IL-6, NO, MPO and MDA were reduced in the mice administered epicatechin, whereas antioxidant enzymes showed increased activity in epicatechin-treated mice and cell line respectively. Furthermore, inhibition effect on NF-κB activation by epicatechin were demonstrated in vivo and in vitro. CONCLUSIONS Epicatechin has inhibitory effect on DSS-induced acute UC. This effect is mainly due to its antioxidant effect and the inhibition of inflammatory molecules related to NF-κB pathway.


Journal of Asian Natural Products Research | 2014

New synthetic method of 8-oxocoptisine starting from natural quaternary coptisine as anti-ulcerative colitis agent.

Zhi-Hui Zhang; Lian-Qiu Wu; An-Jun Deng; Jin-Qian Yu; Zhi-Hong Li; Hai-Jing Zhang; Wen-Jie Wang; Hai-Lin Qin

Quaternary coptisine (1), a natural bioactive quaternary protoberberine alkaloid (QPA), was treated with potassium ferricyanide in aqueous solution of 5 N sodium hydroxide leading to the acquisition of 8-oxocoptisine (2) with much higher yield than reported in the literature. This is the first report of the oxidation of a natural QPA by applying potassium ferricyanide as an oxidant. 8-Oxocoptisine showed significant anti-ulcerative colitis efficacy in vitro with EC50 value being 8.12 × 10− 8 M.


Journal of Asian Natural Products Research | 2015

Two new flavonoids from the roots of Scutellaria baicalensis

Hailin Long; Guo-Ying Xu; An-Jun Deng; Zhi-Hong Li; Lin Ma; Yao Lu; Zhi-Hui Zhang; Feng Wu; Hai-Lin Qin

One new flavone, 5,7-dihydroxy-8,2′,3′,6′-tetramethoxyflavone (1), and one new flavonol, 5,7,6′-trihydroxy-2′-methoxyflavonol (2), were isolated from the roots of Scutellaria baicalensis, and their structures were elucidated on the basis of extensive spectroscopic evidences, especially 1D and 2D NMR and HR-ESI-MS experiments. The structure features of these new compounds lie in the substitution at both C-2′ and C-6′ positions of B-ring by hydroxyl or methoxyl groups.


Journal of Natural Products | 2016

Synthesis and Structure–Activity Relationships of N-Dihydrocoptisine-8-ylidene Aromatic Amines and N-Dihydrocoptisine-8-ylidene Aliphatic Amides as Antiulcerative Colitis Agents Targeting XBP1

Meng Xie; Hai-Jing Zhang; An-Jun Deng; Lian-Qiu Wu; Zhi-Hui Zhang; Zhi-Hong Li; Wen-Jie Wang; Hai-Lin Qin

In this study, natural quaternary coptisine was used as a lead compound to design and synthesize structurally stable and actively potent coptisine analogues. Of the synthesized library, 13 N-dihydrocoptisine-8-ylidene amines/amides were found not only to be noncytotoxic toward intestinal epithelial cells (IECs), but they were also able to activate the transcription of X-box-binding protein 1 (XBP1) targets to varying extents in vitro. Antiulcerative colitis (UC) activity levels were assessed at the in vitro molecular level as well as in vivo in animals using multiple biomarkers as indices. In an in vitro XBP1 transcriptional activity assay, four compounds demonstrated good dose-effect relationships with EC50 values of 0.0708-0.0132 μM. Moreover, two compounds were confirmed to be more potent in vivo than a positive control, demonstrating a curative effect for UC in experimental animals. Thus, the findings of this study suggest that these coptisine analogues are promising candidates for the development of anti-UC drugs.


Acta Pharmaceutica Sinica B | 2016

Three new lignan glucosides from the roots of Scutellaria baicalensis

Hailin Long; Hai-Jing Zhang; An-Jun Deng; Lin Ma; Lian-Qiu Wu; Zhi-Hong Li; Zhi-Hui Zhang; Wen-Jie Wang; Jian-Dong Jiang; Hai-Lin Qin

Three new lignan glucosides, baicalensinosides A–C (1–3), were isolated from the roots of Scutellaria baicalensis. The structural elucidation was achieved by in-depth spectroscopic examinations and qualitative chemical test. Structurally, these compounds belong to the 3,4-dibenzyltetrahydrofuran-type lignan glycoside with a mono-hydroxyl substitution at the 7′-position of benzylidene group on the numbering system of lignans being one of their shared critical features. The anti-osteoporotic activity of the isolated compounds was assessed in an in vitro osteoprotegerin (OPG) transcriptional activity assay using dual luciferase reporter detection. At 10 μmol/L, compounds 1–3 increased the relative activating ratio of OPG transcription to 1.83, 0.84 and 0.98 times that of the control group, respectively.


Molecules | 2017

14,15-Secopregnane-Type Glycosides with 5α:9α-Peroxy and Δ6,8(14)-diene Linkages from the Roots of Cynanchum stauntonii

An-Jun Deng; Jin-Qian Yu; Zhi-Hong Li; Lin Ma; Zhi-Hui Zhang; Hai-Lin Qin

Three new 14,15-secopregnane-type glycosides, stauntosides UA, UA1, and UA2, were isolated from the roots of Cynanchum stauntonii. The three compounds share the first reported and same basic structural features of 3β-hydroxy-14:16,15:20,18:20-triepoxy-5α:9α-peroxy-14,15-secopregnane-6,8(14)-diene named as stauntogenin G as the aglycones. The structures of the new compounds were characterized on the basis of extensive spectroscopic analyses, mainly 1D and 2D NMR and MS methods and chemical analysis. The isolation and identification of the new compounds graced the structural diversity of pregnane-type steroids from C. stauntonii.


Journal of Asian Natural Products Research | 2017

Sugar-free pregnane-type steroids from the roots of Cynanchum stauntonii

An-Jun Deng; Dan Zhang; Qian Li; Zhi-Hui Zhang; Zhi-Hong Li; Hai-Lin Qin

Abstract Two new sugar-free 14,15-secopregnane-type steroids, 14-O-methyl-3-epi-hirundigenin (1) and 2-deoxyamplexicogenin A (2), along with two known sugar-free pregnane-type steroids, were isolated from the 95% ethanol extract of the roots of Cynanchum stauntonii. The structures of the new compounds were characterized on the basis of extensive spectroscopic analyses, mainly 1D and 2D NMR methods, albeit the MS experiments did not display the molecular ion peaks. Compound 2 was the aglycones of stauntosides J and K, etc., previously isolated from C. stauntonii. The isolation and identification of the new compounds graced the structural diversity of pregnane-type steroids from C. stauntonii.


European Journal of Pharmaceutical Sciences | 2017

Development of an XBP1 agonist, HLJ2, as a potential therapeutic agent for ulcerative colitis

Hai-Jing Zhang; Zhi-Hui Zhang; GuangMing Song; XiaoNan Tang; HuaChen Song; An-Jun Deng; Wen-Jie Wang; Lian-Qiu Wu; Hai-Lin Qin

&NA; There is a severe lack of effective treatments for ulcerative colitis (UC), a recurrent and intractable inflammatory bowel disease. The identification of valid targets and new drugs is an urgent need. In this study, we identified the XBP‐1 agonist HLJ2 as a promising treatment candidate. In an in vivo mouse model of DSS‐induced colitis, HLJ2 decreased weight loss, colon contracture, disease activity index (DAI), colon mucosa damage index (CMDI) and histopathological index (HI). HLJ2 also decreased myeloperoxidase (MPO) activity and reduced production of the inflammatory cytokines TNF‐&agr;, IL‐1&bgr;, and IL‐6. HLJ2 improved intestinal mucosa damage induced by dextran sodium sulfate (DSS) and increased the expression of ZO‐1 and claudin‐1. Fecal 16s rRNA high‐throughput sequencing demonstrated a significant improvement in UC intestinal dysbacteriosis in mice treated with HLJ2, including increased abundance of probiotics such as Lachnospiraceae, Prevotellaceae, and Lactobacillaceae. At the same time there was a reduction in the abundance of pathogenic or conditional pathogenic microorganisms such as Bacteroidaceae, Porphyromonadaceae, Deferribacteraceae, and Pseudomonadaceae in HLJ2‐treated mice compared with untreated mice. Our results demonstrated that the XBP1 agonist HLJ2 inhibits inflammation, regulates the intestinal flora, and protects the intestinal mucosa. It is thus a potential therapeutic agent for ulcerative colitis. Graphical Abstract Figure. No caption available.

Collaboration


Dive into the Zhi-Hui Zhang's collaboration.

Top Co-Authors

Avatar

Hai-Lin Qin

Peking Union Medical College

View shared research outputs
Top Co-Authors

Avatar

An-Jun Deng

Peking Union Medical College

View shared research outputs
Top Co-Authors

Avatar

Zhi-Hong Li

Peking Union Medical College

View shared research outputs
Top Co-Authors

Avatar

Lian-Qiu Wu

Peking Union Medical College

View shared research outputs
Top Co-Authors

Avatar

Hai-Jing Zhang

Peking Union Medical College

View shared research outputs
Top Co-Authors

Avatar

Wen-Jie Wang

Peking Union Medical College

View shared research outputs
Top Co-Authors

Avatar

Lin Ma

Peking Union Medical College

View shared research outputs
Top Co-Authors

Avatar

Jin-Qian Yu

Peking Union Medical College

View shared research outputs
Top Co-Authors

Avatar

Qian Li

Peking Union Medical College

View shared research outputs
Top Co-Authors

Avatar

Dan Zhang

Peking Union Medical College

View shared research outputs
Researchain Logo
Decentralizing Knowledge