Zhi-Qiang Gao

Effect of subsoiling in fallow period on soil water storage and grain protein accumulation of dryland wheat and its regulatory effect by nitrogen application.

To provide a new way to increase water storage and retention of dryland wheat, a field study was conducted at Wenxi experimental site of Shanxi Agricultural University. The effect of subsoiling in fallow period on soil water storage, accumulation of proline, and formation of grain protein after anthesis were determined. Our results showed that subsoiling in fallow period could increase water storage in the 0–300 cm soil at pre-sowing stage and at anthesis stage with low or medium N application, especially for the 60–160 cm soil. However, the proline content, glutamine synthetase (GS) activity, glutamate dehydrogenase (GDH) activity in flag leaves and grains were all decreased by subsoiling in fallow period. In addition, the content of albumin, gliadin, and total protein in grains were also decreased while globulin content, Glu/Gli, protein yield, and glutelin content were increased. With N application increasing, water storage of soil layers from 20 to 200 cm was decreased at anthesis stage. High N application resulted in the increment of proline content and GS activity in grains. Besides, correlation analysis showed that soil storage in 40–160 cm soil was negatively correlated with proline content in grains; proline content in grains was positively correlated with GS and GDH activity in flag leaves. Contents of albumin, globulin and total protein in grains were positively correlated with proline content in grains and GDH activity in flag leaves. In conclusion, subsoiling in fallow period, together with N application at 150 kg·hm−2, was beneficial to increase the protein yield and Glu/Gli in grains which improve the quality of wheat.

Variation of Bacterial Community Diversity in Rhizosphere Soil of Sole-Cropped versus Intercropped Wheat Field after Harvest.

As the major crops in north China, spring crops are usually planted from April through May every spring and harvested in fall. Wheat is also a very common crop traditionally planted in fall or spring and harvested in summer year by year. This continuous cropping system exhibited the disadvantages of reducing the fertility of soil through decreasing microbial diversity. Thus, management of microbial diversity in the rhizosphere plays a vital role in sustainable crop production. In this study, ten common spring crops in north China were chosen sole-cropped and four were chosen intercropped with peanut in wheat fields after harvest. Denaturing gradient gel electrophoresis (DGGE) and DNA sequencing of one 16S rDNA fragment were used to analyze the bacterial diversity and species identification. DGGE profiles showed the bacterial community diversity in rhizosphere soil samples varied among various crops under different cropping systems, more diverse under intercropping system than under sole-cropping. Some intercropping-specific bands in DGGE profiles suggested that several bacterial species were stimulated by intercropping systems specifically. Furthermore, the identification of these dominant and functional bacteria by DNA sequencing indicated that intercropping systems are more beneficial to improve soil fertility. Compared to intercropping systems, we also observed changes in microbial community of rhizosphere soil under sole-crops. The rhizosphere bacterial community structure in spring crops showed a strong crop species-specific pattern. More importantly, Empedobacter brevis, a typical plant pathogen, was only found in the carrot rhizosphere, suggesting carrot should be sown prudently. In conclusion, our study demonstrated that crop species and cropping systems had significant effects on bacterial community diversity in the rhizosphere soils. We strongly suggest sorghum, glutinous millet and buckwheat could be taken into account as intercropping crops with peanut; while hulled oat, mung bean or foxtail millet could be considered for sowing in wheat fields after harvest in North China.

Effects of film mulching regime on soil water status and grain yield of rain-fed winter wheat on the Loess Plateau of China

Abstract Shortages and fluctuations in precipitation are influential limiting factors for the sustainable cultivation of rain-fed winter wheat on the Loess Plateau of China. Plastic film mulching is one of the most effective water management practices to improve soil moisture, and may be useful in the Loess Plateau for increasing soil water storage. A field experiment was conducted from July 2010 to June 2012 on the Loess Plateau to investigate the effects of mulching time and rates on soil water storage, evapotranspiration (ET), water use efficiency (WUE), and grain yield. Six treatments were conducted: (1) early mulching (starting 30 days after harvest) with whole mulching (EW); (2) early mulching with half mulching (EH); (3) early mulching with no mulching (EN); (4) late mulching (starting 60 days after harvest) with whole mulching (LW); (5) late mulching with half mulching (LH); and (6) late mulching with no mulching (LN). EW increased precipitation storage efficiency during the fallow periods of each season by 18.4 and 17.8%, respectively. EW improved soil water storage from 60 days after harvest to the booting stage and also outperformed LN by 13.8 and 20.9% in each growing season. EW also improved spike number per ha by 13.8 and 20.9% and grain yield by 11.7 and 17.4% during both years compared to LN. However, EW decreased WUE compared with LN. The overall results of this study demonstrated that EW could be a productive and efficient practice to improve wheat yield on the Loess Plateau of China.