Zhichao Pei
Royal Institute of Technology
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Publication
Featured researches published by Zhichao Pei.
ChemBioChem | 2007
Zhichao Pei; Hui Yu; Matthias Theurer; Annelie Waldén; Peter Nilsson; Mingdi Yan; Olof Ramström
Interactions between carbohydrates and proteins are increasingly being recognized as crucial in many biological processes, such as cellular adhesion and communication. In order to investigate the interactions of carbohydrates and proteins, the development of efficient analytic technologies, as well as novel strategies for the synthesis of carbohydrates, have to be explored. To date, several methods have been exploited to analyze interactions of carbohydrates and proteins, for example, biosensors, nuclear magnetic resonance (NMR); enzyme-linked immunosorbent assays (ELISA), X-ray crystallography and array technologies. This thesis describes the development of novel strategies for the synthesis of carbohydrates, as well as new efficient strategies to Quartz Crystal Microbalance- (QCM-) biosensors and carbohydrate microarrays technologies. These methodologies have been used to probe carbohydrate-lectin-interactions for a range of plant and animal lectins.
Scientific Reports | 2015
Xueming Li; Siyu Song; Qi Shuai; Yihan Pei; Teodor Aastrup; Yuxin Pei; Zhichao Pei
A novel approach to the study of binding thermodynamics and kinetics of carbohydrate-protein interactions on unfixed cancer cell surfaces using a quartz crystal microbalance (QCM) biosensor was developed, in which binding events take place at the cell surface, more closely mimicking a biologically relevant environment. In this study, colon adenocarcinoma cells (KM-12) and ovary adenocarcinoma cells (SKOV-3) grew on the optimized polystyrene-coated biosensor chip without fixation. The association and dissociation between the cell surface carbohydrates and a range of lectins, including WGA, Con A, UEA-I, GS-II, PNA and SBA, were monitored in real time and without label for evaluation of cell surface glycosylation. Furthermore, the thermodynamic and kinetic parameters of the interaction between lectins and cell surface glycan were studied, providing detailed information about the interactions, such as the association rate constant, dissociation rate constant, affinity constant, as well as the changes of entropy, enthalpy and Gibbs free energy. This application provides an insight into the cell surface glycosylation and the complex molecular recognition on the intact cell surface, which may have impacts on disease diagnosis and drug discovery.
Chemical Communications | 2008
Hai Dong; Zhichao Pei; Olof Ramström
An unexpected activation effect from combinations of anionic reagent and amine base resulted in dramatic rate enhancements in multiple carbohydrate cascade inversion.
Polymers | 2017
Siyu Song; Yuchao Lu; Xueming Li; Shoupeng Cao; Yuxin Pei; Teodor Aastrup; Zhichao Pei
Quartz crystal microbalance (QCM) has been extensively applied in real-time and label-free biomolecular interaction studies. However, the sensitive detection by QCM technology remains challenging, mainly due to the limited surface immobilization capacity. Here, a three-dimensional (3D) carboxymethyl dextran coated gold sensor chip surface was successfully fabricated with dextran of different molecular weight (100, 500 and 2000 kDa, respectively). To evaluate the 3D carboxymethyl dextran surface immobilization capacity, the 3D surface was used for studying antigen–antibody interactions on the QCM biosensor. The results showed that the protein immobilization capacity of the 3D carboxymethyl dextran (2000 kDa) surface exceeded more than 4 times the capacity of the 2D carboxyl surface, and 2 times the capacity of the traditional 3D carboxymethyl dextran (500 kDa) surface. Furthermore, the kinetic and affinity properties of antigen–antibody interactions were performed. Most notably, the optimized 3D carboxymethyl dextran (2000 kDa) surface could be used for small molecule detection, where the binding of biotinylated oligo (0.67 kDa) reached 8.1 Hz. The results confirmed that a 3D carboxymethyl dextran (2000 kDa) surface can be exploited for sensitive detection of low molecular weight analytes, which have great potential applications for characterizing the interactions between small molecule drugs and proteins.
Bioorganic & Medicinal Chemistry | 2006
Sabine André; Zhichao Pei; Hans-Christian Siebert; Olof Ramström; Hans-Joachim Gabius
Biosensors and Bioelectronics | 2005
Zhichao Pei; Henrik Anderson; Teodor Aastrup; Olof Ramström
Angewandte Chemie | 2004
Rikard Larsson; Zhichao Pei; Olof Ramström
Chemistry of Materials | 2005
Bernadette Harnish; Joshua T. Robinson; Zhichao Pei; Olof Ramström; Mingdi Yan
Analytical Chemistry | 2007
Yuxin Pei; Hui Yu; Zhichao Pei; Matthias Theurer; Carolin Ammer; Sabine André; Hans-Joachim Gabius; Mingdi Yan; Olof Ramström
Journal of Organic Chemistry | 2007
Hai Dong; Zhichao Pei; Marcus Angelin; Styrbjörn Byström; Olof Ramström