Zhili Xiao
Chinese Academy of Sciences
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Featured researches published by Zhili Xiao.
Molecules | 2008
Jinyi Yang; Hong Wang; Yueming Jiang; Yuanming Sun; Ke Pan; Hongtao Lei; Qing Wu; Yu-Dong Shen; Zhili Xiao; Zhen-Lin Xu
The haptens 4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)carbonyl]-amino]butanoic acid (BFNB) and 6-[((2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)-carbonylamino]hexanoic acid (BFNH) were synthesized and then used to develop a rapid, specific and sensitive ELISA method to determine residues of the pesticide carbofuran in a variety of matrices. A hybridoma cell line (5D3) producing anti-carbofuran monoclonal antibodies (MAbs) was also established. Based on the MAbs in combination with the heterologous hapten BFNH coupled to either horseradish peroxidase (HRP) or ovalbumin (OVA), four ELISAs (formats I-IV) for the quantification of carbofuran were developed and compared. Among them, the optimized format II (the conjugate-coated direct competitive ELISA) showed the best characteristics, with an IC50 value of 18.49 ng/mL, a limit of detection of 0.11 ng/mL and the shortest assay time (1 h). This ELISA method was then applied to the determinations of carbofuran in environmental water, soil and food samples. The relative standard deviations (R.S.D.s) ranged from 1.8% to 21.3% and the mean recoveries were 104.6%, 108.3%, 106.3% and 100.1% for water, soil, lettuce and cabbage, respectively. Thus, the ELISA method of format II exhibited the potential to develop commercial ELISA kits for a rapid detection of carbofuran for human health and environmental safety.
Archive | 2009
Yu-Dong Shen; Shiwei Zhang; Yuanming Sun; Hong Wang; Hongtao Lei; Zhili Xiao; Jinyi Yang
Archive | 2008
Yu-Dong Shen; Yu Wang; Yuanming Sun; Hongtao Lei; Hong Wang; Zhili Xiao; Xiaoyan Xu
Archive | 2009
Yu-Dong Shen; Shiwei Zhang; Yuanming Sun; Hongtao Lei; Hong Wang; Zhili Xiao; Jinyi Yang; Zhaoting Cai
Archive | 2011
Zhili Xiao; Jinyi Yang; Zhaoting Cai; Hongtao Lei; Hong Wang; Yu-Dong Shen; Shiwei Zhang; Yuanming Sun
Archive | 2008
Yu-Dong Shen; Shiwei Zhang; Yuanming Sun; Zhili Xiao; Hongtao Lei; Hong Wang
Archive | 2006
Yuanming Sun; Zhili Xiao; Hongtao Lei; Qing Wu; Suqing Zhao
Archive | 2007
Hongtao Lei; Yuanming Sun; Xiaoyu Huang; Hong Wang; Ke Pan; Qing Wu; Zhili Xiao; Guolian Chen
Archive | 2006
Yuanming Sun; Hongtao Lei; Xiaoyu Huang; Hong Wang; Qing Wu; Guolian Chen; Ke Pan; Zhili Xiao
Archive | 2012
Hongtao Lei; Qing Wu; Guolian Chen; Yuanming Sun; Hong Wang; Yu-Dong Shen; Ke Pan; Jinyi Yang; Zhili Xiao