Zhipeng Liu

Study on Differences of Early-Mid ERPs Induced by Emotional Face and Scene Images

Researches about the cortical processing mechanisms of emotions have important scientific significance and application value. To probe cortical processing differences of emotional face and scene images, electroencephalogram (EEG) of sixteen volunteers was recorded while they watching emotional images. Early-mid occipital ERPs (Event Related Potentials) under different images were compared, and RMS (Root Mean Square) was calculated to analyze activities of the whole brain. Results showed that the N1 (170 ms) amplitudes and P2 (250 ms) amplitudes induced by face images were respectively larger and smaller than that induced by scene images, which embodied specific processing of faces and reprocessing of complex scenes. Negative scene images were processed preferentially and induced more obvious N1 than positive or neutral scene images. Comparisons of ERPs among the whole brain displayed that occipital lobe was the main active region and frontal lobe responsible for emotional regulation was activated mainly at moments of N1 and P2. The early-mid ERPs comparisons explicitly showed cortical processing differences of emotional face and scene images, which deserved further studies.

Relationship Between Perisylvian Essential Language Sites and Arcuate Fasciculus in the Left Hemisphere of Healthy Adults

Essential language sites and the arcuate fasciculus (AF) have been extensively researched. However, the relationship between them remains insufficiently studied, especially in healthy people. Navigated transcranial magnetic stimulation (nTMS) is increasingly used in language mapping. While enjoying the advantage of non-invasiveness, it is also capable of inducing a virtual lesion in the brain. Thus, it offers the possibility of using the virtual-lesion method to study the healthy brain. This study combined nTMS and diffusion tensor imaging (DTI) tractography to investigate the relationship between essential language sites and the AF in 30 healthy right-handed volunteers. A total of 143 essential language sites were identified using nTMS, and a total of 175 AF terminations were identified using DTI tractography. Sixty-six sites had a direct correlation with the AF, accounting for 46% of the total essential language sites. Forty-seven AF terminations harbored essential language sites, accounting for 27% of the total AF terminations. Upon data rendering to the cortical parcellation system, a region-related heterogeneity of the correlation rate was found. This study provides the first data on the relationship between essential language sites and the AF in healthy adults.

Theta and Alpha Oscillations during the Retention Period of Working Memory by rTMS Stimulating the Parietal Lobe

Studies on repetitive transcranial magnetic stimulation (rTMS) have shown that stimulating the parietal lobe, which plays a role in memory storage, can enhance performance during the “retention” process of working memory (WM). However, the mechanism of rTMS effect during this phase is still unclear. In this study, we stimulated the superior parietal lobe (SPL) using 5-Hz rTMS in 26 participants and recorded electroencephalography (EEG) while they performed a delayed-recognition WM task. The analyses included the comparisons of event-related spectral perturbation (ERSP) value variations in theta (4–7 Hz) and alpha (8–14 Hz) band frequencies between conditions (rTMS vs. sham), as well as the correlations between different brain areas. Following rTMS, the ERSP values of theta-band oscillations were significantly increased in the parietal and occipital-parietal brain areas (P < 0.05*), whereas the ERSP values of alpha-band oscillations were significantly decreased in the parietal area (P < 0.05*). The ERSP value variations of theta-band oscillations between the two conditions in the left parietal and left prefrontal areas were positively correlated with the response time (RT) variations (by using rTMS, the more subject RT decreased, the more ERSP value of theta oscillation increased). The ERSP value variations of alpha-band oscillations in the left parietal and bilateral prefrontal areas were negatively correlated with RT variations (by using rTMS, the more RT of the subject decreased, the more ERSP value of alpha oscillation decreased). Inter-sites phase synchronization of theta-band EEG between the left parietal and left prefrontal areas, as well as alpha-band EEG between the left parietal and bilateral prefrontal areas were enhanced by rTMS. These results indicated that activities of both parietal and prefrontal areas were required for information storage, and these activities were related to the behavioral responses. Moreover, the connectivity between these two regions was intensified following rTMS. Thus, rTMS may affect the frontal area indirectly via the frontal parietal pathway.

Simultaneous stimulation using rTMS and tDCS produces the most effective modulation of motor cortical excitability in healthy subjects: a pilot study

Repetitive transcranial magnetic stimulation (rTMS) and transcranial direct current stimulation (tDCS) can be used to modulate the excitability of the cortex, but instances of the two technologies being used to stimulate two positions of the human brain simultaneously are rare. As an initial investigation into the efficacy, feasibility and safety of such an approach, we compared the effects of simultaneously applying rTMS and cathodal tDCS with that of four other stimulation regimens (cathodal tDCS alone, rTMS alone, rTMS after cathodal tDCS, and sham stimulation) on a single population of subjects consisting of five healthy volunteers. Additionally, we also conducted SimNibs simulations of the electric field patterns that combined rTMS and cathodal tDCS would produce in cerebral cortices of the subjects. Compared with baseline levels, motor evoked potentials (MEPs; used here as a surrogate measure of cortical excitability) were significantly increased with all four real stimulation methods (pu2009<u20090.05). Compared with sham measurements, significant increases in MEPs were also observed with rTMS alone (pu2009=u20090.0021), rTMS after tDCS (pu2009=u20090.0004), simultaneous rTMS and tDCS (pu2009<u20090.0001), but not with tDCS alone (pu2009=u20090.4182). We also determined that simultaneous rTMS and cathodal tDCS induced a significant increase in MEPs compared with the baseline or sham at all-time points, and resulted in the largest significant increase in MEPs. Our simulations show that applying cathodal tDCS at the standard stimulation position would cause only a 5.8% increase in the strength of the electric field produced by rTMS when the two techniques are used in conjunction. Our findings in this study indicate that combining rTMS with cathodal tDCS is not only safe, but highly-effective as well.

Frontal Eye Field Involvement in Color and Motion Feature-Based Attention: Single-Pulse Transcranial Magnetic Stimulation

An object can have multiple attributes, and visual feature-based attention (FBA) is the process of focusing on a specific one of them. During visual FBA, the frontal eye field (FEF) is considered to be an important brain area related to the choice of attribute. However, the study of the FEF in FBA remains inadequate. We applied single-pulse transcranial magnetic stimulation (TMS) to the right FEF (rFEF), and designed two independent experimental FBA tasks that each involved two attributes (color and motion), to explore the action time of FEF and the spatial transmission of the FEF signal, respectively. The results of the first experiment showed that when TMS was applied to the rFEF at 100 ms after the target image stimulus began, the subjects’ response time increased significantly compared with the response time in the control trials (in which TMS was applied to the vertex). This indicated that inhibiting the rFEF influenced the progress of visual FBA. The results confirm that the FEF is involved in the early stage of visual attention (at ~100 ms). In the second experiment, TMS was applied at 100 ms after the target image stimulus began. We analyzed the electroencephalogram (EEG) signal after TMS, and found that the electrode signal amplitudes for FC4 (which corresponded to the rFEF) were significantly correlated with the electrode signal amplitudes in the posterior regions. In addition, the amplitude rise of the posterior electrode signal lagged ~50 ms behind that of the FC4. Furthermore, for color and motion, different areas in the posterior brain region were involved in signal transmission. In this study, the application of single-pulse TMS was shown to provide a direct and effective method for research on the FEF, and the combination of TMS and EEG recordings allows a high degree of time resolution, which can provide powerful evidence for research on neural signal transmission.

Non-orthogonal one-step calibration method for robotized transcranial magnetic stimulation

BackgroundRobotized transcranial magnetic stimulation (TMS) combines the benefits of neuro-navigation with automation and provides a precision brain stimulation method. Since the coil will normally remain unmounted between different clinical uses, hand/eye calibration and coil calibration are required before each experiment. Today, these two steps are still separate: hand/eye calibration is performed using methods proposed by Tsai/Lenz or Floris Ernst, and then the coil calibration is carried out based on the traditional TMS experimental step. The process is complex and time-consuming, and traditional coil calibration using a handheld probe is susceptible to greater calibration error.MethodsA novel one-step calibration method has been developed to confirm hand/eye and coil calibration results by formulating a matrix equation system and estimating its solution. Hand/eye calibration and coil calibration are performed to confirm the pose relationships of the marker/end effector ‘X’, probe/end effector ‘Y’, and robot/world ‘Z’. First, the coil is fixed on the end effector of the robot. During the one-step calibration process, a marker is mounted on the top of the coil and a calibration probe is fixed at the actual effective position of the coil. Next, the robot end effector is moved to a series of random positions ‘A’, the tracking data of marker ‘B’ and probe ‘C’ is obtained correspondingly. Then, a matrix equation system AXu2009=u2009ZB and AYu2009=u2009ZC can be acquired, and it is computed using a least-squares approach. Finally, the calibration probe is removed after calibration, while the marker remains fixed to the coil during the TMS experiment. The methods were evaluated based on simulation data and on experimental data from an optical tracking device. We compared our methods with two classical methods: the QR24 method proposed by Floris Ernst and the handheld coil calibration method.ResultsThe new methods outperform the QR24 method in the aspect of translational accuracy and performs similarly in the aspect of rotational accuracy, the total translational error decreased more than fifty percent. The new approach also outperforms traditional handheld coil calibration of navigated TMS systems, the total translational error decreased three- to fourfold, and the rotational error decreased six- to eightfold. Furthermore, the convergence speed is improved 16- to 27-fold for the new algorithms.ConclusionThese results suggest that the new method can be used for hand/eye and coil calibration of a robotized TMS system. Two complex steps can be simplified using a least-squares approach.

Electric Pulses Can Influence Galvanotaxis of Dictyostelium discoideum

Galvanotaxis, or electrotaxis, plays an essential role in wound healing, embryogenesis, and nerve regeneration. Up until now great efforts have been made to identify the underlying mechanism related to galvanotaxis in various cells under direct current electric field (DCEF) in laboratory studies. However, abundant clinical research shows that non-DCEFs including monopolar or bipolar electric field may also contribute to wound healing and regeneration, although the mechanism remains elusive. Here, we designed a novel electric stimulator and applied DCEF, pulsed DCEF (pDCEF), and bipolar pulse electric field (bpEF) to the cells of Dictyostelium discoideum. The cells had better directional performance under asymmetric 90% duty cycle pDCEF and 80% duty cycle bpEF compared to DCEF, with 10u2009Hz frequency electric fields eliciting a better cell response than 5u2009Hz. Interestingly, electrically neutral 50% duty cycle bpEF triggered the highest migration speed, albeit in random directions. The results suggest that electric pulses are vital to galvanotaxis and non-DCEF is promising in both basic and clinical researches.

The Effects of rTMS Combined with Motor Training on Functional Connectivity in Alpha Frequency Band

It has recently been reported that repetitive transcranial magnetic stimulation combined with motor training (rTMS-MT) could improve motor function in post-stroke patients. However, the effects of rTMS-MT on cortical function using functional connectivity and graph theoretical analysis remain unclear. Ten healthy subjects were recruited to receive rTMS immediately before application of MT. Low frequency rTMS was delivered to the dominant hemisphere and non-dominant hand performed MT over 14 days. The reaction time of Nine-Hole Peg Test and electroencephalography (EEG) in resting condition with eyes closed were recorded before and after rTMS-MT. Functional connectivity was assessed by phase synchronization index (PSI), and subsequently thresholded to construct undirected graphs in alpha frequency band (8–13 Hz). We found a significant decrease in reaction time after rTMS-MT. The functional connectivity between the parietal and frontal cortex, and the graph theory statistics of node degree and efficiency in the parietal cortex increased. Besides the functional connectivity between premotor and frontal cortex, the degree and efficiency of premotor cortex showed opposite results. In addition, the number of connections significantly increased within inter-hemispheres and inter-regions. In conclusion, this study could be helpful in our understanding of how rTMS-MT modulates brain activity. The methods and results in this study could be taken as reference in future studies of the effects of rTMS-MT in stroke patients.

Primary Study on the Parameters and Methods to Evaluate Needling Sensation and Maneuvers in Acupuncture

Aim To search the external method of Acupuncture Deqi and Manipulation Methods. Methods develope the experimental equipment to pick up the local myoelectricity signal on the point and volume pulse wave of the tiny artery in the finger-end. By which we can carry out acupuncture on the tested point, and pick up the local myoelectricity signal and volume pulse wave on the point before and after the condition of Deqi and under different manipulation methods. Results primarily deduce the relationship between the two coefficients and the extent of acupuncture Deqi and the reinforcing method and reducing method, that is, when the acupuncture point is under the state of Deqi, the myoelectricity signal of the point occurs, besides the extent of Deqi has a positive direct ratio relation with the intensity and number of the times of the myoelectricity; When carrying out the reinforcing method in acupuncture, the amplitude and frequency of the volume pulse wave decreases; when carrying out the reducing method, it increases. Conclusion the changes of Manipulation Methods and the difference of Acupuncture Deqi can be external evaluated by the local myoelectricity signal and volume pulse wave on the point.