Zhouli Liu

Accumulation and tolerance characteristics of cadmium in a potential hyperaccumulator-Lonicera japonica Thunb

Phytoremediation using hyperaccumulators is a promising technique of removing soil pollutants. In the study, growth responses, cadmium (Cd) accumulation capability and physiological mechanisms of Lonicera japonica Thunb. under Cd stress were investigated. Exposed to 5 and 10 mg L(-1) Cd, the plants did not show any visual symptoms, furthermore, the height, dry biomass of leaves, roots and total and the chlorophyll (CHL) content were obtained different grade increase. When the concentration of Cd was up to 50 mg L(-1), the height, dry biomass of leaves and roots had not significant differences compared with the control. The indexes of tolerance (IT) were all above 0.8. The maintenance of high superoxide dismutase (SOD) and catalase (CAT) activities was observed along with the increased Cd concentration, suggesting strong internal detoxification mechanisms inside plant cells. After 21 days exposure to 25 mg L(-1) Cd, stem and shoot Cd concentrations reached 344.49+/-0.71 and 286.12+/-9.38 microg g(-1) DW, respectively and the plant had higher bioaccumulation coefficient (BC) and translocation factor (TF). According to these results, it was shown L. japonica had strong tolerance and accumulation capability to Cd, therefore it is a potential Cd-hyperaccumulator.

Hormetic Responses of Lonicera Japonica Thunb. To Cadmium Stress

The hormetic responses of Lonicera japonica Thunb. to cadmium (Cd) stress were investigated in a hydroponic experiment. The present results showed that root length and total biomass dry weight increased in comparison with the control at low concentrations Cd. The height of the plant exposed to 2.5 and 5 mg L-1 Cd increased significantly by 11.9% and 12.8% relative to the control, and with the increase of Cd concentrations in the medium, plant height began to decrease. The responses of photosynthetic pigments contents and relative water content to Cd stress had a similar trend, which all showed significantly an inverted U-shaped dose–response curve and confirmed that the stimulatory effect of low concentrations Cd occurred in the plant. Furthermore, L. japonica, as a new Cd-hyperaccumulator, could be considered as a new plant model to study the underlying mechanisms of the hormesis.

ECOTOXICOLOGICAL RESPONSES OF THREE ORNAMENTAL HERB SPECIES TO CADMIUM

Cadmium is one of the most toxic elements. The ideal vegetal cover should be ensured by the selection of appropriate plant species for successful phytoremediation. In the present study, the ecotoxicological effects of Cd on the following 3 ornamental herbs were investigated: Italian ryegrass (Lolium multiflorum Lam.), white clover (Trifolium repens L.), and alfalfa (Medicago sativa L.). Based on the inhibition rate of seed germination, root and shoot elongation, early seedling growth, median inhibition concentration (IC50) values, and index of tolerance (IT) values, ecotoxicological indicators were determined. The results showed that 10 μM to 50 μM Cd had little effect on seed germination or root and shoot elongation of the 3 ornamental herbs (p > 0.01). With an increase in Cd concentrations, alfalfa (M. sativa) was the most sensitive to Cd toxicity in terms of seed germination and root elongation. Based on the IC50 of root elongation, Italian ryegrass (L. multiflorum) was the least sensitive to Cd. Based on the IC50 of seed germination and shoot elongation, white clover had the least sensitivity to Cd. Among the 3 ornamental herbs, based on the IC50 of seed germination and root and shoot elongation, alfalfa (M. sativa) was all the most sensitive plant. According to the index of tolerance, Italian ryegrass (L. multiflorum) was the most tolerant plant.

Regulatory Effects of Elevated Carbon Dioxide on Growth and Biochemical Responses to Ozone Stress in Chinese Pine (Pinus tabulaeformis Carr.)

This study examined whether carbon dioxide (CO2) might alleviate ozone (O3) injury to the dominant coniferous forest species of northern China, Pinus tabulaeformis Carr. After 90 days O3 exposure, biomass and net photosynthetic rate (Pn) decreased significantly by 24.44 % and 42.89 % compared with the control. A significant increase in malondialdehyde (MDA) was shown, suggesting cell membrane damage and oxidative stress. However, the positive responses of biomass dry weight, antioxidative enzymes and soluble sugar contents under elevated CO2 alone and the combination of elevated CO2 and O3 were observed, indicating that CO2 could ameliorate O3-induced injury. The study provided increasing evidence that moderately elevated CO2 levels may have a beneficial effect on the forest ecosystem to respond to global climate change.

Effects of cadmium on growth and accumulation characteristics in leaf bud of Lonicera Japonica Thunb.

Cadmium (Cd) pollution has become a global concern as Cd has some adverse effects on plants or other life forms. Lonicera japonica Thunb. is a typical ornamental plant of vertical gardening and has been showed as a potential Cd-hyperaccumulator. In this study, effects of Cd on growth and accumulation characteristics in leaf bud of Lonicera Japonica Thunb. were detected. After 21 days exposure to 5 and 10 mg L-1 Cd, there were no significant differences in leaf bud fresh biomass and dry biomass of L. japonica. Exposed to 25 mg L-1 Cd, the leaf bud of L. japonica did not induce significant damage. The good response of leaf bud biomass and water content could be related with the high tolerance of the plant to Cd. Furthermore, a positive linear correlation between Cd accumulation in leaf bud and Cd concentration in the medium was shown, indicating L. japonica may have a good accumulation ability exposed to higher Cd concentration in the medium.

The 2nd International Conference on Water Resource and Environment (WRE 2016)

Table of contents01 The eco-toxicological effects of cadmium stress on five ornamental plantsZhouli Liu, Wei Chen, Xingyuan He, Shuai Yu, Weihang Ding02 Study on the formation and release mechanisms of hydrogen sulfide in Longtan coal mineJianjun Liu, Yuliang Wu, Kaixiang Fan03 Coral bleaching in the North Reef of China’s Xisha Islands in 2014Yuanchao Li, Xinqing Zheng, Shiquan Chen, Haiqun Xie04 Soil chemical characteristics in a Cunninghamia lanceolata stand suffering from ice-snow damageJie Li, Zhuomin Wang, Li Xue05 Diversity of understory vegetation was under a Cunninghamia lanceolata stand suffering from ice-snow damageZhiyang Lie, Weilong Huang, Tongtong Zhou, Li Xue06 The effect of water, nitrogen and harvesting time on yield and biomass allocation of Helianthus tuberosus L.Kai Gao, Tiexia Zhu07 The vertical variation of microbial communities in the sediment of sea cucumber pondWeiteng Mao, Pei Yu, Xinfang Lv, Yunchun Li08 Indicating significances of alpine plant functional groups to environmental changePeixi Su, Rui Shi09 Research on the daily CH4 fluxes of soil in summer mangrove community at Yingluogang of Guangxi, ChinaYong Jiang, Xingbin Chen, Shichu liang10 Beach protection structures in middle and lower reaches of Yangtze RiverJunfeng Xu11 Social responsibility management of large dam project with owner as core: an empirical case in ChinaJiwei Zhu, Zhao Zhai, Linan Zhou, Yun Le, Li Cao12 The ecological function value assessment analysis of urban waterfrontJiwei Zhu, Ping Lu, Zhiqian Huang, Bei Zhou13 Mechanism of diurnal osmotic potential changes and adjustment of three Caragana species in different habitats of the Inner Mongolia PlateauZhirong Zheng, Shihai Lv, Chaoyang Feng14 Biodiversity of phytoplankton and environmental influences analysis of Longfeng Wetland, ChinaNan Song, Yan Liu, Xinxin Lu, Yawen Fan15 The effect of human activities on culturable soil microbes from Yaoquan Mountain in Wudalianchi, ChinaJianfei Guan, Jihua Wang, Xueping Zhang

Biomass accumulation and nutrient uptake of 16 riparian woody plant species in Northeast China

Our research focused on eutrophication control and species screening for riparian zone vegetation restoration in the upstream reach of the Hun River. We studied 16 hardwood plant species to investigate nutrient concentrations and nitrogen and phosphorus accumulations. After about 120 days of growth in pots, these 16 species varied in dry matter biomass, ranging from 15.13 to 637.16 g. Total nitrogen (TN) and total phosphorus (TP) concentrations and distribution in roots, stems and foliage differed both within and between tested species. Mean TN and TP accumulation ranged from 0.167 to 14.730 g per plant and from 0.016 to 1.20 g, respectively. All 16 species, but especially Lespedeza bicolor, Robinia pseudoacacia and Sorbaria sorbifolia had strong potential to remove TN and TP from soil and could be widely utilized for the restoration of destroyed riparian zones in northeast China.

Effects of temperature on gas exchange characteristics in leaves of Lonicera Japonica Thunb effects of temperature on gas exchange

The temperature which affects plant growth and development, is an important ecological factor. In the present study, we mainly aimed to study the effects of temperature on gas exchange characteristics in the leaves of Lonicera Japonica Thunb., The results showed that light elevated temperature had no significant effects on the photosynthesis of L. japonica (Lonicera Japonica), which was well indicated by the responses of net photosynthesis rate (PN), stomatal conductance (Gs), intercellular CO2 concentration (Ci) and transpiration rate (E). Intercellular CO2 concentration and transpiration rate had a good relationship with stomatal conductance. The study will provide a reference for L. japonica to response temperature warming and be useful for environmental protection.