Zhu Li

The Nucleotide Sequence Diversity Analysis of the Pig MyoG Gene

The MyoG gene was sequenced in 63 individuals from 10 breeds (eight Chinese domestic breeds, one recently derived breed and one introduced breed) of pigs in this experiment to analyze the nucleotide sequence diversity of the MyoG gene. The following conclusions can be drawn from the experiment. In the complete sequence of the pig MyoG gene, the content of C+G base is significantly higher than that of A+T. And the high content of G and C base in the coding region caused that bias. In the coding region of the MyoG gene, the G and C base are abundant at the 1st and the 3rd site of the codon, especially at the 3rd site. The high content of G and C base is beneficial for the stability of the MyoG gene. Generally speaking, the MyoG gene is very conservative, the composition and construction of the coding region are very stability, but mutations can be found in noncoding region. Transversion happens with higher frequency than that of transition, and transition happens mainly between T and C base, transversions mainly between G and C base. In the flanking region and intronic region, the MyoG gene has relatively high nucleotide diversity. These mutations exist in noncoding region caused the nucleotide sequence diversity of the MyoG gene.

Notice of Retraction Comparative study of the nucleotide sequence of MyoG gene between pig and 11 different species

Utilizing direct sequencing technique, a consensus nucleotide sequence of pig MyoG gene was got from 63 individuals in this experiment. At the same time, the MyoG gene sequences of 11 different species were collected from Genbank. The coding sequence and deduced amino acid sequences of these species were compared to study the genetic diversity and phylogeny of MyoG gene between pig and other species. The results indicated that the identity of amino acid of MyoG gene was high, but compared with mammal in all of these 5 detected fishes there were three similar extra sequence fragments existing near 260, 300 and 740 site of the coding sequence. Phylogenetic tree of different species were constructed from the coding sequence of MyoG gene. The species grouped into two lineages, one consisted of mammalians include human being, swine, cattle and mouse; another consisted of 5 different kinds of fish. Chicken formed an independent branch on the tree but was closer to mammals. The results were coincident with the traditional taxonomy conclusion. From this study, we can conclude that some highly conservative sequence obtained from functional gene can be used in the phylogeny study to reconstruct the phylogenetic tree between some far species in the taxonomy.