Ziya Dumlupinar

Geographical description and molecular characterization of genetic structure and diversity using 6K SNP Array in Turkish oat germplasm

Abstract: Cultivated oat (Avena sativa L. and Avena byzantina Koch) is native to Turkey, a secondary center of oat diversity. Oat breeding has received less attention relative to other cereals. In this work, the diversity of oat landraces collected from different regions of Turkey as well as obtained from different gene banks was investigated using 3293 high quality SNP markers. Expected heterozygosity (Hs), observed heterozygosity (Ho), inbreeding coefficient (Fis), and overall genetic diversity (Ht) of the oat gene pool were 0.22, 0.01, 0.96, and 0.38, respectively. The value of the genetic differentiation (Fst) metric for genetic structure was 0.41 and indicated that kinship was more of a determinant for population structure than the geographical provenance. The populations from different geographical regions shared a great proportion of genetic diversity. Clustering using model-based STRUCTURE, principal coordinates (PCoA), and neighbour-joining (NJ) algorithms were mostly comparable except for five discrepantly clustered accessions in NJ and PCoA relative to STRUCTURE, which can be attributed to the relatively reduced resolution power in the NJ and PCoA approaches. SNP markers partitioned all oat accessions into four main groups (A, B, C, and D) with 10 unclassified accessions. Some landraces were identical based on genetic distance and can represent duplications in gene banks. The data presented in this work represent the initial results on genetic diversity as investigated in Turkish oat, and are an important resource for systematicians, geneticists, and breeders interested in Turkish oat germplasm. These results are expected to open new opportunities for further studies in oat genomics and cultivar development.

Bazı Ticari ve Yerel Yulaf Genotiplerinin Çimlenme ve Fide Gelişimi Üzerine Tuz Stresinin Etkileri

Bu calisma, bazi ticari ve yerel yulaf genotiplerinin cimlenme ve erken fide gelisim donemlerinde olusturulan tuz stresine tepkilerini belirlemek amaciyla, Duzce Universitesi, Cilimli Meslek Yuksekokulu laboratuvarinda 2016 yilinda yurutulmustur. Arastirmada, 10’u tescilli, 6 yerel olmak uzere 16 yulaf genotipine, 0 (kontrol), 50 ve 100 mM dozlarinda tuz (NaCl) stresi uygulanmistir. Arastirmada genotiplerin, 24. Saatteki su alim orani (%), cimlenme yuzdeleri (%), kok uzunluklari (cm), surgun uzunluklari (cm), kok yas agirliklari (mg/bitki), surgun yas agirliklari (mg/bitki), kok kuru agirliklari (mg/bitki), surgun kuru agirliklari (mg/bitki), tuz dayaniklilik indeksi (%) ve tohum guc indeksleri degerleri incelenmistir. Incelenen tum ozellikler yonuyle genotipler, tuz dozlari ve genotip x tuz dozu interaksiyonlari istatistiksel olarak onemli bulunmustur. Genotiplerin incelenen ozellikler bakimindan tuzluluga tepkileri farkli olmustur. Artan tuz konsantrasyonlari yulaf genotiplerinin hem cimlenmesini hem de fide gelisimini olumsuz yonde etkilemistir. Tuz dayaniklilik indeksi degerleri bakimindan TL558, Arslanbey ve Faikbey genotipleri tuza dayanikli olarak one cikmislardir.