Zoltán Péter

Activation of transient receptor potential vanilloid-3 inhibits human hair growth.

In the current study, we aimed at identifying the functional role of transient receptor potential vanilloid-3 (TRPV3) ion channel in the regulation of human hair growth. Using human organ-cultured hair follicles (HFs) and cultures of human outer root sheath (ORS) keratinocytes, we provide the first evidence that activation of TRPV3 inhibits human hair growth. TRPV3 immunoreactivity was confined to epithelial compartments of the human HF, mainly to the ORS. In organ culture, TRPV3 activation by plant-derived (e.g., eugenol, 10-1,000 μM) or synthetic (e.g., 2-aminoethoxydiphenyl borate, 1-300 μM) agonists resulted in a dose-dependent inhibition of hair shaft elongation, suppression of proliferation, and induction of apoptosis and premature HF regression (catagen). Human ORS keratinocytes also expressed functional TRPV3, whose stimulation induced membrane currents, elevated intracellular calcium concentration, inhibited proliferation, and induced apoptosis. Of great importance, these effects on ORS keratinocytes were all mediated by TRPV3, as small interfering RNA-mediated silencing of TRPV3 effectively abrogated the cellular actions of the above agonists. These findings collectively support the concept that TRPV3 signaling is a significant player in human hair growth control. Therefore, TRPV3 and the related intracellular signaling mechanism might function as a promising target for pharmacological manipulations of clinically relevant hair growth disorders.

Role of acellular dermal matrix allograft in minimal invasive coverage of deep burn wound with bone exposed--case report and histological evaluation.

A sandwich graft was applied to the debrided cortical bone layer of the tibia in the case of a 72‐year‐old male patient with full‐thickness necrotic burn injury. The combined graft consisted of a dermal template material and autologous split thickness skin graft. After application, the graft was found totally accepted and provided good functionality with acceptable appearance. Histopathologic evaluation revealed a complete take with revascularisation of the implant. Supporting lamellar bony trabecules were also seen in the deep dermal dermis representing a connection to the underlying bone. The use of the dermal matrix in deep burn exposing the bone provides a satisfactory functional result and good cosmetic appearance.

Long-Term Followup of Dermal Substitution with Acellular Dermal Implant in Burns and Postburn Scar Corrections

Full-thickness burn and other types of deep skin loss will result in scar formation. For at least partial replacement of the lost dermal layer, there are several options to use biotechnologically derived extracellular matrix components or tissue scaffolds of cadaver skin origin. In a survey, we have collected data on 18 pts who have previously received acellular dermal implant Alloderm. The age of these patients at the injury varied between 16 months and 84 years. The average area of the implants was 185 cm2. Among those, 15 implant sites of 14 patients were assessed at an average of 50 months after surgery. The scar function was assessed by using the modified Vancouver Scar Scale. We have found that the overall scar quality and function was significantly better over the implanted areas than over the surrounding skin. Also these areas received a better score for scar height and pliability. Our findings suggest that acellular dermal implants are especially useful tools in the treatment of full-thickness burns as well as postburn scar contractures.

Detection of factor XIII-A is a valuable tool for distinguishing dendritic cells and tissue macrophages in granuloma annulare and necrobiosis lipoidica

Factor XIII subunit A (FXIII‐A) is used as a diagnostic marker in a wide range of dermatological diseases ranging from inflammatory lesions to malignancies, although neither the cell types responsible for its expression nor the mechanism(s) resulting in its local accumulation in pathological conditions have been characterized.

Diagnostic accuracy of 18F-FDG-PET/CT in early and late stages of high-risk cutaneous malignant melanoma

The precise role of total body 18F‐fluorodeoxyglucose‐positron emission tomography/computed tomography (PET/CT) in the clinical management of patients with cutaneous malignant melanoma (CMM) is not well established.

[Colonoscopy, the primary tool for colonic screening].

Through an overview of the colonic screening methods, the authors study colonoscopy with high emphasis. Taking into consideration its high sensitivity and specificity in the detection of colorectal carcinomas and adenomas, as well as the possibility of adenoma removal through examination, colonoscopy is recommended to be applied as the primary colonic screening within the scope of a national screening program.

Immunotopographical Differences of Human Skin

The immunological barrier of the healthy skin is considered to be unified on the whole body surface—however, recent indirect findings have challenged this dogma since microbial and chemical milieu (e.g., sebum, sweat, and pH) exhibit remarkable differences on topographically distinct skin areas. Therefore, in the present study, we performed whole transcriptomic and subsequent pathway analyses to assess differences between sebaceous gland rich (SGR) and sebaceous gland poor (SGP) regions. Here, we provide the first evidence that different skin regions exhibit a characteristic innate and adaptive immune and barrier milieu as we could detect significantly increased chemokine (CCL2, 3, 19, 20, 23, 24) and antimicrobial peptide (S100A7, A8, A9, lipocalin, β-defensin-2) expression, altered barrier (keratin 17, 79) functions, and a non-inflammatory Th17/IL-17 dominance in SGR skin compared to SGP. Regarding pro-inflammatory molecules (IL-1α, IL-6, IL-8, IL-33, TNF-α), similarly low levels were detected in both regions. Our data may explain the characteristic topographical localization of some immune-mediated and autoimmune skin disorders and we also propose that the term “healthy skin control sample,” widely used in experimental Dermatology, should only be accepted if researchers carefully specify the exact region of the healthy skin (along with the site of the diseased sample).

Cell therapy for burns – Our experiences in Debrecen

NAGY ENDRE DR.1,2, ERDEI IRÉN DR.1,2,3, FARKAS MÁTÉ DR.1,2, PÉTER ZOLTÁN DR.1,2, VÁRVÖLGYI TÜNDE DR.1, KIRÁLY GÁBOR DR.1,4, JUHÁSZ ISTVÁN DR.1,2,4 Debreceni Egyetem Általános Orvostudományi Kar, Bőrgyógyászati Tanszék, Debrecen1 Debreceni Egyetem Klinikai Központ, Bőrgyógyászati Klinika Égésiés Bőrsebészeti Osztály2 Debreceni Egyetem Általános Orvostudományi Kar, Aneszteziológiai és Intenzív Terápiás Tanszék3 Debreceni Egyetem Fogorvostudományi Kar, Fogorvosi Műtéttani Koordináló Tanszék4

Nonmelanoma bőrtumorok kezelési lehetőségei szervtranszplantált betegeknél egy esetismertetés kapcsán

Absztrakt A szerzők egy 59 eves ferfi esetet mutatjak be, akinel előszor 1983-ban, majd 2000-ben vegeztek vesetranszplantaciot. Az első veseatultetest kovetően ket evvel jelent meg az első laphamsejtes carcinoma a bőron, majd 2003-tol evente legalabb kettő praecancerosus laesio vagy nonmelanoma bőrdaganat sebeszeti eltavolitasa tortent. Ezek a tumorok elsősorban a napfenynek kitett bőrteruleteken jelentek meg, multiplexen. Tekintettel arra, hogy agressziv viselkedesűek lehetnek es recidivara hajlamosak, komplex kezelest alkalmaztak a betegnel, amely magaba foglalta az immunszuppresszio soran alkalmazott gyogyszerek megvaltoztatasat, illetve mezőterapiak alkalmazasat. Az eset bemutatasa kapcsan a szerzők attekintik ezeket a kezelesi lehetősegeket, hangsulyozva, hogy nemcsak a bőrdaganatok minel korabbi felismerese es aktiv kezelese szukseges, hanem a betegek megfelelő fenyvedelemre valo oktatasa es bőrgyogyaszati gondozasa is a bőrtumorok kialakulasanak megelőzese erdekeben. Orv. Hetil., 2016, 157(24), 971...The authors present the case of a 59-year-old male patient, whose first kidney transplantation was in 1983 and the second in 2000. The first squamous cell carcinoma appeared on the skin 2 years after the first transplantation. Since 2003, at least two precancerous lesions or non-melanoma skin tumors have been removed surgically yearly. These cancers appeared predominantly on the sun-exposed skin, and were multiple. As these tumors could behave aggressively and prone to recurrence, complex treatment was applied, which included a switch in immunosuppressive drugs and the application of field therapies. The authors give an overview of these treatment options in relation to the case presentation, emphasizing that not only early detection and active treatment of the precancerous lesions and skin cancers are essential, but education of proper sun-protection methods and dermatology care are also important in order to avoid the development of these tumors.

[Tomato peel: rare cause of biliary tract obstruction].

Foreign bodies in the biliary tree are rare causes of obstructive jaundice. Food bezoars are infrequent as well. They can cause biliary obstruction after biliary tract interventions, or in the presence of biliary-bowel fistula or duodenum diverticulum. Food bezoars usually pass the gastrointestinal tract without any symptoms, but they can cause abdominal pain and obstructive jaundice in the case of biliary tract obstruction. Endoscopic retrograde cholangio-pancreatography has the major role in the diagnosis and the treatment of the disease. Authors summarize the medical history of a 91-year-old female patient, who developed vomiting and right subcostal pain due to the presence of tomato peel within the ductus choledochus.