Zoran Kulišić

Cryptosporidium infection in lambs and goat kids in Serbia.

The prevalence of Cryptosporidium infection among lambs and goat kids in Serbia was examined. The animals were grouped according to age, i.e., up to 30 days and from 31 to 90 days. The infection was diagnosed using three coprological procedures. Sheather’s sugar flotation was employed to determine the concentration of oocysts. Modified Ziehl-Neelsen technique and modified Kinyoun technique were used as staining procedures. Cryptosporidium oocysts were detected in 42.1% examined lambs and 31.8% goat kids. These findings clearly demonstrate the presence of Cryptospodium infection in lambs and goat kids in Serbia and indicate the potential role of these animals as reservoirs of cryptosporidia.

First records of Dirofilaria repens in wild canids from the region of Central Balkan.

Dirofilaria repens causes an emerging zoonotic disease in Europe, particularly in its southern part, the Mediterranean region. Many reports on human dirofilariosis have been published recently, but little is known about the wildlife hosts and reservoirs of this parasite in nature. This paper presents the first records of adult D. repens specimens from free-ranging carnivores in Central Balkan countries (Serbia and Macedonia). During the period 2009-2013, a total of 145 regularly shot canids were examined for the presence of D. repens adults. In order to investigate their role as hosts and potential wild reservoirs of this zoonosis, 71 wolves (Canis lupus), 48 foxes (Vulpes vulpes) and 26 jackals (Canis aureus) were examined. Under the skin of two wolves (one from Serbia and one from Macedonia) and of a red fox from Serbia D. repens adults were found. In all three cases only one parasite was present. Further research on wild canids is needed, particularly on species widening their range (such as jackals) and those living near human settlements (foxes and jackals), which facilitates the transmission of the parasites to dogs and humans.

Scarabidae - intermediate host for Macracanthorhynchus hirudinaceus.

ltd. za kontrolu kvaliteta, Beograd KW Macracanthorhynchus hirudinaceus % coleopteras % swines % epysootiology KR nema Macracanthorhynchus hirudinaceus infestation is parasitosis caused by Macracanthorhynchus hirudinaceus Adult forms parasite in small intestine of swine. Development of parasite is happening through intermediate hosts - coleopteras from Scarabaeidae family (Melolontha vulgaris, Cetonia aurata, Polyphilla fullo, Anomalia vitis etc). Infection begins when swines ingest infected coleopteras. Macracanthorhynchus hirudinaceus infestation is encountered in swines in extensive breeding, as well as in wild boars.

Frequency of antimicrobial resistance in thermophilic campylobacter strains from humans, poultry and pigs

Uþestalost antimikrobne rezistencije termofilnih Campylobacter sojeva poreklom od ljudi, živine i svinja

Molecular Evidence of Q Fever Agent Coxiella Burnetii in Ixodid Ticks Collected from Stray Dogs in Belgrade (Serbia)

Abstract Q fever is a zoonotic disease caused by Coxiella burnetii, a gram-negative coccobacillus, which has been detected in a wide range of animal species, mostly domestic ruminants, but also in wild mammals, pets, birds, reptiles, arthropods (especially ticks), as well as in humans. Although the exposure to domestic animals in rural areas is regarded as the most common cause of the disease in humans, recent studies have shown that the role of pets in the epidemiology of Q fever has been increasingly growing. Although the primary route of infection is inhalation, it is presumed that among animals the infection circulates through ticks and that they are responsible for heterospecifi c transmission, as well as spatial dispersion among vertebrates. The aim of this study was to determine the presence and prevalence of C. burnetii in ticks removed from stray dogs, as well as to examine the distribution of tick species parasitizing dogs on the territory of Belgrade city. A PCR protocol targeting IS1111 repetitive transposon-like region of C. burnetii was used for the detection of C. burnetii DNA in ticks and the results were confi rmed by sequence analysis. In total, 316 ticks were collected from 51 stray dogs - 40 females (78.43%) and 11 males (21.57%). Three species of ticks were identifi ed: Rhipicephalus s anguineus (72.15%), Ixodes ricinus (27.53%) and Dermacentor reticulatus (0.32%). Out of 316 examined ticks, C. burnetii DNA was detected only in the brown dog tick R. sanguineus, with a total prevalence of 10.53% (24/228) . The high prevalence of C. burnetii in R. sanguineus, which is primarily a dog tick, indicates the importance of dogs in the epidemiology of Q fever in the territory of Belgrade.

ZASTUPLJENOST DIROFILARIA IMMITIS, BORRELIA BURGDORFERI, ANAPLASMA SPP. I EHRLICHIA SPP. KOD NEVLASNIČKIH PASA NA PODRUČJU BEOGRADA

Bolesti pasa koje se prenose vektorima predstavljaju oboljenja virusne, bakterijske ili parazitske etiologije, koja se najcesce prenose hematofagnim artropodama, komarcima i krpeljima. Cilj ovog rada bio je da se ispita prisustvo infekcija ciji se uzrocnici prenose komarcima (DiroGilaria immitis) i krpeljima (Borrelia burgdorferi, Anaplasma phagocytophilum/A. platys, Ehrlichia canis/E. ewingii) kod nevlasnickih pasa na teritoriji Beograda. U periodu od septembra do decembra 2015. godine sakupljeno je 86 uzoraka krvi od klinicki zdravih nevlasnickih pasa iz razlicitih beogradskih opstina. Svi uzorci testirani su modiNikovanim Knott-ovim testom i komercijalnim SNAP® 4Dx Plus® Test-om. Infekcija izazvana barem jednim od ispitivanih uzrocnika ustanovljena je kod 27,91% pasa. Najzastupljeniji uzrocnik bila je D. immitis, koja je dijagnostikovana kod 17,44% životinja. Na prisustvo antitela protiv Anaplasma spp. pozitivno je bilo 9,30% pasa, dok je 2,33% jedinki bilo seropozitivno na B. burgdorferi, odnosno na Ehrlichia spp. Koinfekcije sa dva patogena zabeležene su kod 3,49% pasa. Rezultati ovog istraživanja pokazuju da su nevlasnicki psi na teritoriji grada Beograda izloženi delovanju razlicitih vrsta zoonoznih uzrocnika koji se prenose komarcima i krpeljima, zbog cega treba preduzeti odgovarajuce mere za suzbijanje ovih artropoda.

Canine demodicosis in territory of city of Niš

This paper describes cases of demodicosis in dogs from the territory of the city of Nis. The objective of the investigations was to learn more about the parameters that can influence the susceptibility of certain dogs to the cause of demodicosis, such as: age, hair length, sex, and seasonal dynamics of the incidence of the disease. It was established following examinations of 76 dogs suspected of demodicosis that they were all infected with the specie Demodex canis. The changes were localized or generalized. In the total number of infected dogs, there was a larger share of males (61.84%) than females (38.16%). The number of dogs diseased with demodicosis was twice higher in shorthaired than in long-haired animals. The most frequently infected were dogs of the Doberman and German Shepherd breeds. The number of male and female animals among short-haired dogs diseased with demodicosis was equal, while there were more males among the long-haired animals. The biggest number of infected animals was in the category of young dogs, up to one year of age, and they accounted for 65.79% of the total number of diseased animals, which shows without any doubt that younger dogs are more susceptible to demodicosis. There were more infected dogs in the period autumn-winter than in the period spring-summer.

Heteracidosis in pheasants

Diseases of parasite etiology present a great part of the pheasant game pathology in both natural domiciles and artificial breeding. Based on the research conducted in our country and abroad, we have come to the conclusion that heteracidosis, along with coccidiosis, syngamosis, and ascaridiosis present one of the most significant parasite diseases in these birds. Heteracidosis in pheasants is caused by Heterakis isolonche and H. gallinarum. According to our findings H. isolonche is detect in 26,1 % of pheasants aged from 4 to 14 weeks, and 14,4 % of adult pheasants in organized maintenance conditions, and in 18,1 % of pheasants in nautural domiciles. Hetarkis gallinarum is found in 25,1 % of pheasants aged from 4 to 14 weeks and 4,3 % of adult pheasants in pheasant farms, and 15,3 % pheasants in natural domiciles. For these reasons, in this paper we present the carriers, pathological changes and clinical picture of the disease, as well as prevention and control methods.