Zuoqing Song

Advanced research on anti-tumor effects of amygdalin

Malignant tumors are the major disease that cause serious damage to human health, and have been listed as the premier diseases which seriously threatened human health by World Health Organization (WHO). In recent years the development of antitumor drugs has been gradually transformed from cytotoxic drugs to improving the selectivity of drugs, overcoming multidrug resistance, development of new targeted drugs and low toxicity with high specificity drugs. Amygdalin is a natural product that owns antitumor activity, less side effects, widely sourced and relatively low priced. All these features make the amygdalin a promising antitumor drugs, if combined with conditional chemotherapy drugs, which can produce synergistic effect. In this paper, we summarized the pharmacological activity, toxicity and antitumor activity of amygdalin, mainly focused on the advanced research of amygdalin on its antitumor effects in recent years, providing new insights for the development of new anticancer drugs, new targets searching and natural antitumor mechanism investigations.

Primary Multiple Pulmonary Primitive Neuroectodermal Tumor: Case Report and Literature Review.

AbstractPrimitive neuroectodermal tumors (PNETs) arising directly from the lung are extremely rare but particularly aggressive neoplasms. Although thoracic PNET usually develops on the chest wall, there have been reports of primary Ewing sarcoma/PNET of the lung.We present the case of a 16-year-old male with PNET diagnosed following histologic and immunohistochemical examination of a video-assisted thoracic surgical biopsy. As typically occurring for these tumors, the diagnosis was initially delayed in our patient and prognosis was poor despite aggressive surgical resection and postoperative chemotherapy. We attempted to explore the use of targeted pharmacotherapy through high-throughput sequencing in this case. We found that this treatment using Avastin and Cetuximab could provide a new therapeutic direction for PNET.PNENTs arising from the lung parenchyma without pleural or chest wall involvement are extremely rare. Although uncommon, if the pathological features are similar to Ewing sarcoma, PNENTs should be kept in mind, and the target therapy may be a potent treatment for this disease.

Primary Endobronchial Plasmacytoma Involving Local Lymph Nodes and Presenting with Rare Immunoglobulin G Lambda Monoclonal Gammopathy

Extramedullary plasmacytoma occurring as a primary pulmonary lesion is rare. The present report describes a 42-year-old Chinese man diagnosed with primary pulmonary plasmacytoma following left lower lobectomy. Of note, an extremely rare immunoglobulin G lambda paraprotein was documented in the patients serum by immunofixation electrophoresis. The patient has been well, showing no local recurrence or multifocal disease during a 15-month follow-up.

[The expression and clinical significance of heparanase in non-small cell lung cancer.].

BACKGROUND It has been proven that heparanase played an important role in tumorigenesis. The aim of this work is to investigate the level of expression of heparanase protein in non-small cell lung cancer (NSCLC) and to explore the relationship between heparanase expression and NSCLC histological types and prognosis. METHODS Immunohistochemical method was used to detect the heparanase expression in the routinely paraffin-embedded sections of surgical specimen of 66 cases with NSCLC and 15 ones with normal lung tissues. RESULTS Heparanase was highly expressed in lung cancer tissues (74.2%) while negative in epithelia of normal lung tissues. The expression of heparanase was significantly correlated with TNM stage (P =0.042) and lymphatic metastasis (P =0.005). The patients with positive heparanase expression had a significantly shorter survival rate than those with negative heparanase expression (P =0.025). CONCLUSIONS Heparanase has more expression in NSCLC than normal lung tissues. It was correlated with TNM stage and lymphatic metastasis and prognosis of NSCLC. However, heparanase expression is not an independent prognostic factor.

Sarcoidosis misdiagnosed as malignant tumors: a case report

BackgroundSarcoidosis is a rare condition that is often misdiagnosed as malignant tumors due to the similar clinical manifestations and imaging findings.Case PresentationWe encountered a 56-year-old Chinese woman who had a chief complaint of a persistent cough. The chest computer tomography (CT) revealed mediastinal and bilateral hilar lymph node enlargement, and positron emission tomography-computer tomography (PET-CT) revealed abnormal fluorodeoxyglucose (FDG) uptake in the lymph nodes of the chest and abdomen. To further clarify the diagnosis, a lymph node sampling was performed by video-assisted thoracoscopic surgery (VATS) and the histopathologic diagnosis of sarcoidosis was confirmed.ConclusionsVATS could be an effective and minimally invasive diagnostic method to discriminate pulmonary sarcoidosis with other malignant tumors.

Association between clinical pathology and multiple genes mRNA expression in Chinese patients with NSCLC

OBJECTIVE The aim of this study was to evaluate whether there was an association between pathology type and ERCC1, BRCA1, RRM1, TUBB3, STMN1, TOP2A and epidermal growth factor receptor (EGFR) messenger ribonucleic acid (mRNA) expression level in Chinese patients with non-small cell lung carcinoma (NSCLC). MATERIALS AND METHODS mRNA expression level of these genes was analyzed in 181 cancer tissues by using xTAG-step liquid-chip array. The mRNA expression level of the seven genes was evaluated in association with the clinical pathology type. RESULTS The average mRNA expression level of the seven genes were ERCC1 (1.02 ± 0.03), BRCA1 (0.15 ± 0.04), RRM1 (0.19 ± 0.05), TUBB3 (0.31 ± 0.06), STMN1 (2.78 ± 0.42), TOP2A (3.04 ± 0.42) and EGFR (0.58 ± 0.09), respectively in Chinese patients with NSCLC. The mRNA expression level of ERCC1, STMN1 and TOP2A genes were statistical different with different pathology type (p(a) < 0.05); STMN1 and TOP2A genes mRNA expression were much higher in squamous cell lung carcinoma than that in non-squamous cell lung carcinoma (p(a) < 0.05). And ERCC1 gene expression was much lower in squamous cell carcinoma than that in non-squamous cell carcinoma (p(a) < 0.05). CONCLUSION mRNA expression level of STMN1, TOP2A and ERCC1 were correlated with the clinical pathology type.

Synchronous primary intrapulmonary and mediastinal thymoma-A case report

We report an extremely rare case of Synchronous primary intrapulmonary and mediastinal thymoma in a Chinese patient. We describe the histological and radiological findings, which support the possibility of multicentric thymoma. Resection of the mass in the left anterior superior mediastinum and upper lobectomy of right lung were performed, with lymph Nodes clearance, superior vena cava, left and right brachiocephalic veins resection, reconstruction of left brachiocephalic vein to right auricle and reconstruction of right brachiocephalic vein to superior vena cava.

[Identification of the offspring of Vegfr2-luc transgenic mouse].

BACKGROUND AND OBJECTIVE A transgenic mouse, Vegfr2-luc, in which a luciferase reporter (luc) is under control of the murine VEGFR2 promoter, can be used to track angiogenesis in vivo. The aim of this study is to identify the offspring of Vegfr2-luc transgenic mouse. METHODS Luc was detected with PCR in genomic DNA of the new-born mouse. Luc expression in the offspring of Vegfr2-luc transgenic mouse was monitored with IVIS in vivo imaging system during post-natal development. Wound-healing models of Vegfr2-luc transgenic mouse offspring were established and the expression of luc was monitored during the wound-healing process. Luc activity and VEGFR2 mRNA expression in different organs were detected with luc Assay System and Real-time PCR respectively. RESULTS PCR showed that 50% (56/112) of the offspring of Vegfr2-luc transgenic mouse carry luc. IVIS in vivo imaging results demonstrated that luc expression in Vegfr2-luc transgenic mouse dropped dramatically with age increase (P<0.001) and luc expression in the wound first increased and then decreased during the wound-healing process (P<0.001). Luc activity in female Vegfr2-luc transgenic mouse organs was positively correlated with VEGFR2 mRNA expression (r=0.948, P<0.001). Except testis, luc activity in male Vegfr2-luc transgenic mouse organs was also positively correlated with VEGFR2 mRNA expression (r=0.836, P<0.001). CONCLUSION The offspring of Vegfr2-luc transgenic mouse is applicable to tracking angiogenesis in vivo.

[Expression and Clinical Significance of Heparanase and Ki-67 in Non-small Cell Lung Cancer.].

BACKGROUND It has been proven that heparanase and Ki-67 had relationship with occurrence and metastasis in lung cancer. The aim of this work was to investigate the expressions of heparanase and Ki-67 and to explore their clinical significance and mutual relations in non-small cell lung cancer (NSCLC). METHODS Immunohistochemical method was used to detect the heparanase and Ki-67expression in the routinely paraffin-embedded sections of surgical specimen of 70 cases with NSCLC and 20 ones with normal lung tissues. RESULTS Heparanase and Ki-67 were highly expressed in lung cancer tissues (72.9% and 85.7%) while negative in epithelia of normal lung tissues. The expression of heparanase was significantly correlated with TNM stage (P=0.044) and lymphatic metastasis (P=0.001). The expression of Ki-67 was significantly correlated with tumor size (P=0.03) and histology type (P=0.001) and differentiation (P=0.01) and lymphatic metastasis (P=0.01) and TNM stage (P=0.043). The expression of heparanase and Ki-67 was no significant difference (P=0.323). CONCLUSIONS Heparanase and Ki-67 were involved in the occurrence and development of lung cancer and they may predict patients prognosis, while the expression of heparanase and Ki-67 had no association with NSCLC, which may contribute to the different mechanisms involved in tumor occurrence and development.

Role of artemin in non-small cell lung cancer: Function of artemin in lung cancer

In this study, we investigated the role of artemin, a member of the glial cell‐derived neurotrophic factor of ligands, in the malignant phenotype of lung cancer.