A. Clode

Cytogenetic investigations of 340 thyroid hyperplasias and adenomas revealing correlations between cytogenetic findings and histology

Cytogenetic analyses were performed on 340 follicular thyroid adenomas and goiters after short-term culture. Clonal chromosomal changes were found in 67 cases. Trisomy 7 as the sole abnormality or along with other trisomies was the most frequent type of aberration (19 cases). Other recurrent numerical changes were loss of chromosome 22 (4 cases) and the second X or the Y chromosome (5 cases). Translocations involving 19q13 (12 cases) were frequent structural chromosomal changes. Dicentric chromosomes or telomeric associations were frequent in goiters (12 cases). After a histopathologic classification of all cases, we have correlated the cytogenetic findings with the histology of the tumors. Only 8.4% of the goiters showed clonal abnormalities, whereas 44.9% of the adenomas revealed clonal abnormalities. Furthermore, simple clonal changes were predominantly found in goiters and complex changes in adenomas. The most impressive correlation was found in the group of lesions with trisomy 7. Although all but one lesion with one or two additional trisomies were goiters, those having three or more additional trisomies were all adenomas or adenomatous goiters.

Cytogenetic findings in 18 follicular thyroid adenomas

Cytogenetic study of 18 follicular thyroid adenomas showed clonal chromosome changes in 12 tumors. These results suggest the existence of at least three cytogenetically distinct subgroups: a hyperploid group characterized by the presence of a cluster of numerical changes including +5, +7, and +12 as the most frequent anomalies and, less frequently, +4, +9, +14, +16, and +17; a pseudo- or near-diploid group characterized by simple karyotypic aberrations; and a cytogenetically normal group.

Follicular thyroid carcinoma: Chromosome analysis of 19 cases

Short‐term cultures of 19 follicular thyroid carcinomas were examined cytogenetically. Clonal chromosomal changes were detected in 12 tumors. Two follicular carcinomas had only numerical alterations: one with a hyperdiploid karyotype with trisomies/polysomies of chromosomes 7 and 12, similar to the karyotypes previously identified in a sub‐group of benign thyroid lesions, and the other with monosomy 20. In the remaining ten cases several structural chromosome anomalies were found. Loss of the short arm of chromosome 3 was observed in one tumor. In two widely invasive and metastasizing follicular carcinomas there was a t(7;8)(p15;q24) as the sole abnormality in one case and a der(8)t(7;8)(p15;q24) together with other cytogenetic alterations in the other case. This finding suggests that t(7;8)(p15;q24) may be related to an aggressive behavior of follicular thyroid carcinomas. Genes Chromosomes Cancer 21:250–255, 1998.

A kindred with multiple endocrine neoplasia type 2A associated with pruritic skin lesions.

Background. A kindred affected by multiple endocrine neoplasia type 2A (MEN 2A), associated with symmetric, bilateral, scapular pruritic skin lesions (PSL), is reported.

Alveolar adenoma of the lung: further characterization of this uncommon tumour

Alveolar adenoma of the lung is a poorly characterized, uncommon pulmonary lesion with proliferation of alveolar epithelium and septal mesenchyme. We describe the electron microscopy, immunohistochemistry and DNA flow cytometry in a case of alveolar adenoma in a 55-year-old woman. Alveolar adenoma appears to be a distinct benign neoplasm of the alveolar structures. Our findings further suggest that it is not a precursor of bronchioloalveolar carcinoma or other type II pneumocyte lesions of presumed malignant potential.

Mucoepidermoid Carcinoma of Major and Minor Salivary Glands A Survey of 43 Cases With Study of Prognostic Indicators

Forty-three consecutive cases of mucoepidermoid carcinoma of the major and minor salivary glands were studied to evaluate the influence of the following parameters on prognosis: age, sex, location, local extension, mitotic counts, vascular invasion, grade of differentiation, and nuclear DNA ploidy assessed by image cytometry. The age of the patients ranged between 7 and 84 years. Twenty-eight patients were male and 15 female. Thirty-five tumors were in the major salivary glands and 8 in the minor glands of the oral cavity. According to Healeys criteria, 14 tumors were classified as grade 1, 17 as grade 2, and 12 as grade 3 and, according to the 1990 World Health Organization proposal, 17 tumors were classified as low-grade and 26 as high-grade neoplasms. Image cytophotometric DNA analysis revealed 15 euploid and 6 aneuploid tumors. The range of the follow-up study was 3-156 months (mean, 40.7 months). The 5- year actuarial survival for each of the above-cited parameters was: 100% and 59.1 % for patients aged less than 20 and over 55 years, respectively; 49.6% for male and 85.7% for female patients; 61.3% for major gland and 73.3% for minor gland tumors; 100% and 57.5% for intraglandular and extraglandular major salivary gland neo plasms, respectively; 46.2% for cases with high mitotic rate and 83.1% for those with low mitotic rate; 38.7% and 81.6% for tumors respectively with and without vascular invasion; 100% for grade 1 and 64.7% for grade 2; none of the patients with grade 3 neoplasms were alive at 5 years. Survival was of 100% for low-grade and 40.7% for high-grade tumors. Survival was 92.8% for euploid tumors and 61.3% for the aneuploid ones. In conclusion, survival of patients with mucoepidermoid carcinoma is significantly related to the histological grade of differentiation, the mitotic count, the presence of vascular invasion and the DNA ploidy of the tumor cells. Int J Surg Pathol 1(1):3-12, 1993