A.G.J.M. van Leeuwen

Visualizing breast cancer using the Twente photoacoustic mammoscope: what do we learn from twelve new patient measurements?

We acquired images of breast malignancies using the Twente photoacoustic mammoscope (PAM), to obtain more information about the clinical feasibility and limitations of photoacoustic mammography. Results were compared with conventional imaging and histopathology. Ten technically acceptable measurements on patients with malignancies and two measurements on patients with cysts were performed. In the reconstructed volumes of all ten malignant lesions, a confined region with high contrast with respect to the background could be seen. In all malignant cases, the PA contrast of the abnormality was higher than the contrast on x-ray mammography. The PA contrast appeared to be independent of the mammographically estimated breast density and was absent in the case of cysts. Technological improvements to the instrument and further studies on less suspicious lesions are planned to further investigate the potential of PAM.

Photoacoustic image patterns of breast carcinoma and comparisons with Magnetic Resonance Imaging and vascular stained histopathology

Photoacoustic (optoacoustic) imaging can visualize vasculature deep in tissue using the high contrast of hemoglobin to light, with the high-resolution possible with ultrasound detection. Since angiogenesis, one of the hallmarks of cancer, leads to increased vascularity, photoacoustics holds promise in imaging breast cancer as shown in proof-of-principle studies. Here for the first time, we investigate if there are specific photoacoustic appearances of breast malignancies which can be related to the tumor vascularity, using an upgraded research imaging system, the Twente Photoacoustic Mammoscope. In addition to comparisons with x-ray and ultrasound images, in subsets of cases the photoacoustic images were compared with MR images, and with vascular staining in histopathology. We were able to identify lesions in suspect breasts at the expected locations in 28 of 29 cases. We discovered generally three types of photoacoustic appearances reminiscent of contrast enhancement types reported in MR imaging of breast malignancies, and first insights were gained into the relationship with tumor vascularity.

Multiple and dependent scattering effects in Doppler optical coherence tomography

Doppler optical coherence tomography (OCT) is a technique to image tissue morphology and to measure flow in turbid media. In its most basic form, it is based on single (Mie) scattering. However, for highly scattering and dense media multiple and concentration dependent scattering can occur. For Intralipid solutions with varying scattering strength, the effect of multiple and dependent scattering on the OCT signal attenuation and Doppler flow is investigated. We observe a non-linear increase in the OCT signal attenuation rate and an increasingly more distorted Doppler OCT flow profile with increasing Intralipid concentration. The Doppler OCT attenuation and flow measurements are compared to Monte Carlo simulations and good agreement is observed. Based on this comparison, we determine that the single scattering attenuation coefficient micros is 15% higher than the measured OCT signal attenuation rate. This effect and the distortion of the measured flow profile are caused by multiple scattering. The non-linear behavior of the single scattering attenuation coefficient with Intralipid concentration is attributed to concentration dependent scattering.

Probing drifting and nulling mechanisms through their interaction in PSR B0809+74

Both nulling and subpulse drifting are poorly understood phenomena. We probe their mechanisms by investigating how they interact in PSR B0809+74. We find that the subpulse drift is not aliased but directly reflects the actual motion of the subbeams. The carousel-rotation time must then be over 200 s, which is much longer than theoretically predicted. The drift pattern after nulls differs from the normal one, and using the absence of aliasing we determine the underlying changes in the subbeam-carousel geometry. We show that after nulls, the subbeam carousel is smaller, suggesting that we look deeper in the pulsar magnetosphere than we do normally. The many striking similarities with emission at higher frequencies, thought to be emitted lower too, confirm this. The emission-height change as well as the striking increase in carousel-rotation time can be explained by a post-null decrease in the polar gap height. This offers a glimpse of the circumstances needed to make the pulsar turn off so dramatically.

Null-induced mode changes in PSR B0809+74

We have found that there are two distinct emission modes in PSR B0809+74. Beside its normal and most common mode, the pulsar emits in a signicantly dierent quasi-stable mode after most or possibly all nulls, occasionally for over 100 pulses. In this mode the pulsar is brighter, the subpulse separation is less, the subpulses drift more slowly and the pulse window is shifted towards earlier longitudes. We can now account for several previously unexplained phenomena associated with the nulling-drifting interac- tion: the unexpected brightness of the rst active pulse and the low post-null driftrate. We put forward a new interpretation of the subpulse-position jump over the null, indicating that the speedup time scale of the post-null drifting is much shorter than previously thought. The speedup time scale we nd is no longer discrepant with the time scales found for the subpulse-drift slowdown and the emission decay around the null.

Determination of the scattering anisotropy with optical coherence tomography

In this work we demonstrate measurements with optical coherence tomography (OCT) of the scattering phase function in the backward direction and the scattering anisotropy parameter g. Measurements of the OCT attenuation coefficient and the backscattering amplitude are performed on calibrated polystyrene microspheres with a time-domain OCT system. From these measurements the phase function in the backward direction is determined. The measurements are described by the single scattering model and match Mie calculations very well. Measurements on Intralipid demonstrate the ability to determine the g of polydisperse samples and, for Intralipid, g = 0.35 ± 0.03 is measured, which is well in agreement with g from literature. These measurements are validated using the Intralipid particle size distribution determined from TEM measurements. Measurements of g and the scattering phase function in the backward direction can be used to monitor changes in backscattering, which can indicate morphological changes of the sample or act as contrast enhancement mechanism.

LOFAR observations of PSR B0943+10: profile evolution and discovery of a systematically changing profile delay in Bright mode

We present broadband, low-frequency (25-80 MHz and 110-190 MHz) LOFAR observations of PSR B0943+10, with the goal of better illuminating the nature of its enigmatic mode-switching behaviour. This pulsar shows two relatively stable states: a Bright (B) and Quiet (Q) mode, each with different characteristic brightness, profile morphology, and single-pulse properties. We model the average profile evolution both in frequency and time from the onset of each mode, and highlight the differences between the two modes. In both modes, the profile evolution can be well explained by radius-to-frequency mapping at altitudes within a few hundred kilometres of the stellar surface. If both B and Q-mode emission originate at the same magnetic latitude, then we find that the change of emission height between the modes is less than 6%. We also find that, during B-mode, the average profile is gradually shifting towards later spin phase and then resets its position at the next Q-to-B transition. The observed B-mode profile delay is frequency-independent (at least from 25-80 MHz) and asymptotically changes towards a stable value of about 0.004 in spin phase by the end of mode instance, much too large to be due to changing spin-down rate. Such a delay can be interpreted as a gradual movement of the emission cone against the pulsars direction of rotation, with different field lines being illuminated over time. Another interesting explanation is a possible variation of accelerating potential inside the polar gap. This explanation connects the observed profile delay to the gradually evolving subpulse drift rate, which depends on the gradient of the potential across the field lines.

Unusual subpulse modulation in PSR B0320+39

We report on an analysis of the drifting subpulses of PSR B0320+39 that indicates a sudden step of ~180 degrees in subpulse phase near the centre of the pulse profile. The phase step, in combination with the attenuation of the periodic subpulse modulation at pulse longitudes near the step, suggests that the patterns arise from the addition of two superposed components of nearly opposite drift phase and differing longitudinal dependence. We argue that since there cannot be physical overlap of spark patterns on the polar cap, the drift components must be associated with a kind of “multiple imaging” of a single polar cap “carousel” spark pattern. One possibility is that the two components correspond to refracted rays originating from opposite sides of the polar cap. A second option associates the components with emission from two altitudes in the magnetosphere.

Ontogenetic specialism in predators with multiple niche shifts prevents predator population recovery and establishment

The effects of ontogenetic niche shifts on community structure and dynamics are underexplored, despite the occurrence of such shifts in the majority of animal species. We studied the form of niche shifts in a predator that exhibits multiple ontogenetic niche shifts, and analyzed how this life history complexity affects the size-structured predator-prey dynamics in the system. The predator represents either an ontogenetic generalist, exhibiting a partial shift to predation (in which case an alternative resource is also available) or an ontogenetic specialist, exhibiting a complete shift (in which case the predator depends entirely on predation). We showed two effects on community dynamics from accounting for a complete niche shift to predation: (1) occurrence of alternative stable community states (coexistence and a prey-only community state) and (2) occurrence of deterministic extinction following initially successful invasion (predators can invade an equilibrium with only prey, but are bound to go extinct after a few generations). Both phenomena are due to the match or mismatch in the timing of predators and suitably sized prey and the growth trajectory of the predator, which is plastic, due to the population feedback on available resources. In the case of persistence without invasion (alternative stable community states), slow growth during the pre-piscivorous life stage is necessary to stay in tune with the prey cycle; in the case of extinction following invasion, slow growth through the pre-piscivorous life stage causes the predator to reach the completely piscivorous stage when there is no prey available to feed upon. Somatic growth rates are directly coupled to food availability, which, in turn, is the result of density-dependent feedbacks in the system. Since they primarily determine these density-dependent feedbacks, the ontogenetic niche shifts in predator life history structure the community to a major extent.

‖v‖: new insight into the circular polarization of radio pulsars

We present a study of single pulses from nine bright northern pulsars to investigate the behaviour of circular polarization, V. The observations were conducted with the Effelsberg 100-m radio telescope at 1.41 and 4.85 GHz and the Westerbork radio telescope at 352 MHz. For the first time, we present the average profile of the absolute circular polarization |V| in the single pulses. We demonstrate that the average profile of |V| is the distinguishing feature between pulse components that exhibit low V in the single pulses and components that exhibit high V of either handedness, despite both cases resulting in a low mean. We also show that the |V| average profile remains virtually constant with frequency, which is not generally the case for V, leading us to the conclusion that |V| is a key quantity in the pulsar emission problem.