A. Lakshmana Rao

Development of RP-HPLC Method for the Estimation of Rabeprazole in Pure and Tablet Dosage Form

A simple, rapid, sensitive and precise High Performance Liquid Chromatographic (HPLC) method has been developed for the estimation of rabeprazole in bulk and tablet dosage form. In this method RP-C18 column (150 mm x 4.6 mm I.D, 5 µ m particle size) with mobile phase consisting of methanol and water in the ratio of 65:35 v/v in isocratic mode was used. The detection wavelength is 284 nm and the flow rate is 0.8 mL/min. Tinidazole is used as internal standard. In the range of 0.25-20 µ g/mL, the linearity of rabeprazole shows a correlation coefficient of 0.9999. The drug and internal standard were eluted at 4.41± 0.05 and 2.16± 0.04 min. respectively. The intra- and inter-day variation was found to be less than 1% showing high precision of the assay method. The detection limit was found to be 100 ng/mL. The mobile phase selected for the proposed method is simple, fast, accurate and precise and hence can be applied for routine quality control analysis of rabeprazole in bluk and its tablet dosage from.

Development and Validation of HPTLC Method for the Simultaneous Estimation of Gemifloxacin Mesylate and Ambroxol Hydrochloride in Bulk and Tablet Dosage Form

Abstract A new simple high performance thin layer chromatography method for simultaneous determination of gemifloxacin mesylate and ambroxol hydrochloride in bulk and tablet dosage form were investigated. Chromatographic separation of the drugs was performed on aluminum plates precoated with silica ge160 F254 as a stationary phase and solvent system consisted of acetone:methanol:toluene:formic acid (4:2:3:1 v/v/v/v). Densitometric evaluation of the separated zones was performed at 252nm and the method was validated. The Rf values and drug content of gemifloxacin mesylate and ambroxol hydrochloride were 0.41±0.02, 0.68±0.02 and 100.1 %, 99.84 %. The calibration curves of peak area versus concentration, which was linear from 400–2200 ng/band for gemifloxacin mesylate, 100–550 ng/band for ambroxol hydrochloride and regression coefficient (r2) was greater than 0.99. LOD for gemifloxacin mesylate and ambroxol hydrochloride was 30 and 25 ng/band respectively, while LOQ was 100 and 70 ng/band respectively. The method was validated for linearity, accuracy, robustness as per International Conference on Harmonisation (ICH) guidelines. The study shows that the developed method is simple and accurate and it was found to be suitable for the simultaneous determination of gemifloxacin mesylate and ambroxol hydrochloride in bulk and pharmaceutical formulations.

Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method

An accurate and precise HPLC method was developed for the determination of lisinopril. Separation of the drug was achieved on a reverse phase C8 column using a mobile phase consisting of phosphate buffer and methanol in the ratio of 35:65v/v. The flow rate was 0.8 mL/min and the detection wavelength was 215 nm. The linearity was observed in the range of 20-60 μ g/mL with a correlation coefficient of 0.9992. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of lisinopril in tablet dosage forms.

RP-HPLC Estimation of Trospium Chloride in Tablet Dosage Forms

A rapid, sensitive and precise HPLC method was developed for the estimation of trospium chloride in pure and tablet dosage forms. Seperation of the drug was achieved on a reverse phase Azilent C18 column using a mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 60:40v/v. The flow rate was 1 ml/min and the detection wave length 215 nm. The linearity was found in the range of 10-150 μg/ml with a correlation coefficient of 1.0000. The proposed method was validated for its sensitivity, linearity, accuracy and precision. This method was employed for routine quality control analysis of trospium chloride in tablet dosage forms.

Development and Validation for Simultaneous Estimation of Proguanil and Atovaquone by using RP-HPLC

Proguanil (Figure 1) is a biguanide derivative and exerts its antimalarial action by inhibiting parasitic dihydrofolate reductase enzyme. Proguanil is chemically (1E)-1-[amino-(4-chloroanilino) methylidene]-2-propan-2-yl guanidine. It is converted to an active metabolite called cycloguanil and it has causal prophylactic and suppressive activity against P. falciparum and cures the acute infection. It is also effective in suppressing the clinical attacks of vivax malaria. Proguanil inhibits the dihydrofolate reductase of plasmodia and thereby blocks the biosynthesis of purines and pyrimidines, which are essential for DNA synthesis and cell multiplication [1]. This leads to failure of nuclear division at the time of schizont formation in erythrocytes and liver.

Synthesis, Characterization and Antimicrobial Activities of Copper Derivatives of NHC-II Complexes

BACKGROUND Caffeine, 1, 3, 7-trimethylxanthine is one of the xanthine derivatives that are for the most part utilized as a part of solutions as diuretics. The Cu (II) complexes have been synthesized from the N-heterocyclic carbene ligands. MATERIALS AND METHODS The Cu (II) NHC complexes were characterized using analytical and spectral techniques. Antibacterial and antifungal activities of the Cu (II) NHC complexes were determined using the reported techniques. The SOD activity was assayed using nitrobluetetrazolium as O2 scavenger. RESULTS The X-band ESR spectra of the copper complexes in DMSO solution at 300 and 77 K were recorded and their salient features are reported. The in vitro biological screening effects of the investigated compounds were tested against the bacterial species, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas aeruginosa and fungal species, Aspergillus niger, Rhizopus stolonifer, Aspergillus flavus, Rhizoctonia bataicola and Candida albicans by serial dilution method. CONCLUSION The Cu (II) complexes exhibit square planar geometry. A comparative study of inhibition values of the individual metals and their complexes indicate that the complexes exhibit higher antimicrobial activity than the individual metals. Superoxide dismutase and reducing power activities of the copper complexes have also been studied. Depending on the molecular structure, the Cu (II) NHC complex possess promising SOD mimetic activities. Further we are trying to explore more biological properties of Cu (II) NHC complexes in vitro and in vivo.

Bioanalytical Method Development and Validation for Simultaneous Estimation of Paracetamol and Cefixime by using RP-HPLC in Rabbit Plasma

A novel approach was used to develop and validate a bioanalytical RP-HPLC method for the simultaneous estimation of Paracetamol and Cefixime in rabbit plasma using Cefaclor as internal standard. Evaluation of the content of drugs were done by employing a mixture of Phosphate buffer (p H 6.4) and Acetonitrile (80:20, v/v) as the mobile phase and measure the absorbance at 245 nm for Paracetamol and Cefixime. Retention time was established to be 3.618 min for Cefaclor, 4.608 min for Paracetamol and 5.914min for Cefixime. The results shown that the analytical technique furnished here establishes acceptable accuracy and precision, shorter and easy sample preparation, reduced the complications for equipment on satisfactory analysis time.

Method Development and Validation for Simultaneous Estimation of Rizatriptan Benzoate and Naproxen Sodium by UV Spectrophotometric Method

A Simple, validated, accurate and precise simultaneous UV Spectrophotometric method has been developed for the simultaneous estimation of Rizatriptan Benzoate (RIZ) and Naproxen Sodium (NAP) in pharmaceutical dosage form. Rizatriptan Benzoate exhibits absorption maximum at 282.9nm and Naproxen Sodium shows absorption maximum at 261.8nm in methanol. The Beers law obeyed the concentration range of 5–30μg/ml for RIZ and NAP. Mean recovery of 100.25% for RIZ and 99.2%5 for NAP respectively signifies the accuracy of the method. This method can be used for the routine simultaneous estimation of RIZ and NAP in pharmaceutical dosage forms.