A. V. Boiko

Optical properties of solutions consisting of albumin and γ -globulin molecules in different ratio modeling blood serum

Rayleigh-Debye laser light scattering (RDLS) was used for study of the alterations of physical properties of albumin andγ-globulin molecules water solved in different ratios as a model system of blood serum. By using a portable optical device we simultaneously measured a number parameters of protein solutions such as the effective mass, coefficient of intermolecular interaction, diffusion coefficients of the components, etc. The results of the investigations for model systems were compared with the data obtained for blood serum of healthy and oncology patients.

Multiparametric testing of blood protein solutions with diagnostic purpose

The multiparametric physical method for diagnosis and test of curing cancer diseases based on Rayleigh light scattering is proposed. There was studied simultaneously dynamic and static parameters of blood plasma and serum proteins. A special device for this purpose is described and clinical results are presented.

Laser light scattering study of blood serum proteins: γ-globulin in the solutions containing K + and Na + ions

Static and dynamic light scattering are used to measure molecular parameters of γ-globulin in solutions in the presence of sodium and potassium ions. Intermolecular interactions coefficients and molecular masses of scattering particles in the protein water solutions were determined as a function of pH and ioning strength by static light scattering. In the proteins solutions containing Na+, molecular mass of scattering particles is constant, but in the case of proteins solutions containing K+ ions molecular mass of scattering particles increases in vicinity of isoelectric points of proteins Translational diffusion coefficient dependence as a function of pH and concentration of components was obtained in γ-globulin solutions by using photon correlation spectroscopy.

Physical methods for studying the effect of europium ions on lysozyme solutions

Metals play an important role in a human organism. Concentrations of metals, particularly, heavy metals, exceeding the maximum allowable level in the organism are dangerous for persons. At present, it is of topical interest to study molecular mechanisms of the effect of heavy metal ions on biological fluids, for instance, blood, and to develop sufficiently simple and reliable physical methods for environmental monitoring of the occurrence of heavy metals in natural environments, in the first place, in water.

Static and dynamic light scattering studies of collagen solutions

Static and dynamic light scattering are used to measure molecular parameters of collagen in solutions. Isoelectric point, coefficients of intermolecular interactions and molecular weight of type I collagen were determined by static light scattering. Using photon correlation spectroscopy we obtained the dependence of the translational diffusion coefficient as a function of pH and concentration of collagen in solutions.

Changing optical properties of blood serum proteins in case of oncological diseases

Molecular methods of diagnostics of widespread diseases including oncological pathology on the base static and dynamic laser light scattering in serum blood solution are testified. Rayleigh - Debye laser light scattering method are used to measure molecular parameters of blood serum. Dynamical parameters of macromolecules can be measured by photon correlation spectroscopy method. We obtained that the parameter of intermolecular interaction B for serum blood solution of oncological patients is considerably less then B for serum blood solution of healthy persons and in a number of cases has even a negative value. The effective mass of scattering particles in serum water solutions for samples of oncology diseases patients increase in comparison to control samples. As follows from our experimental results, there is the difference between dynamic molecular parameters for control samples and ones with oncological pathology.

Mobility and intermoleculare interactions of blood serum proteins in the solutions containing the ions with different ionic radii

Mobility and intermoleculare interaction of blood serum proteins - in water solutions containing the ions with different ionic radii were investigated by Rayleigh-Debye light scattering (RDLS), photon-correlation spectroscopy (PCS) and fluorescence methods.

Physical methods for studying the effect of copper and cadmium ions on protein solutions

Using two physical methods (Rayleigh light scattering and Dynamic light scattering), the effect of cooper and cadmium, toxic heavy metals, on proteins in water solutions (including blood serum proteins) was studied. A physical mechanism of interaction between heavy metal ions and charged biopolymers which is based on formation of strong bonds of metal ions with the surface of macromolecules is discussed. Based on the results obtained, a method to monitor natural liquid media pollution with heavy metals is proposed.

Dynamic properties of collagen molecules in water and salt solutions

Dynamic light scattering is used to measure molecular parameters of collagen in solutions. Using photon correlation spectroscopy we obtained the dependences of the translational diffusion coefficient on pH in water and salt solutions. Supermolecular aggregates formation was found in salt solutions (with KCl and Pb(CH3COO)2).

Laser light-scattering diagnostic of blood protein solutions

Molecular methods of diagnostics of widespread diseases including vascular pathology on the base static and dynamic laser light scattering in serum blood solution are testified. The alterations of molecular parameters of blood serum of animal species (rats) after experimentally induced cerebral ischemia (hypoxia) and haemorrhagic stroke relative to controls were studied. It was obtained that effective mass of scattering particles in blood serum solutions is diminished for haemorrhagic and ischemic rats in comparison to control. The relative protein concentrations in blood serum also change both after false operation and in case of induced ischemia.