Yurii M. Petrusevich

Method of light-scattering measurement in tumor diagnostics

The simple, inexpensive and effective multiparametric physical method for diagnosing oncological diseases based on Rayleigh light scattering is proposed. Dynamic and static parameters of plasma blood proteins in dilute water solutions with different pH and ionic strength were studied simultaneously for the first time.

Electrostatic interaction in biopolymer solutions investigated by NMR and laser light scattering

In solution the surface of protein macromolecules is always charged and so the electrostatic interaction between the polar solvent and charged groups on the biopolymer surface is strong enough. It is of interest to consider three types of interaction: protein - protein, protein - contrion, protein - solvent. All of these interactions can change as static parameters of macromolecules solution. This paper deals with the most suitable methods for measurement of these parameters. The methods are NMR (nuclear magnetic resonance) and laser light scattering.

Dynamics of collagen molecules in aqueous solutions containing metal ions with different ionic radii

The dynamic parameters of collagen molecules in aqueous solutions were assessed by the photon-correlation spectroscopy method. The dependences of translational diffusion coefficients as a function of pH in pure aqueous collagen solutions and in solutions containing salts of different metals such as Na, Ca, K, Pb were established. It has been reported that the size of a metal’s ionic radius affects intermolecular interactions and the mobility of collagen molecules in solution.

Static and dynamic light scattering studies of collagen solutions

Static and dynamic light scattering are used to measure molecular parameters of collagen in solutions. Isoelectric point, coefficients of intermolecular interactions and molecular weight of type I collagen were determined by static light scattering. Using photon correlation spectroscopy we obtained the dependence of the translational diffusion coefficient as a function of pH and concentration of collagen in solutions.

Protein aggregation processes in solutions containing heavy metal and chelate ions: studying by laser light scattering and polarized fluorescence methods

The formation of macromolecular protein clusters in the presence of some heavy ions was observed by the Rayleigh light scattering and polarized fluorescence methods. Luminescence spectra of europium chelate and BAS-europium chelate water solutions were obtained at different pH and concentrations of components. The rotational correlation time of particles in the solutions containing heavy ions increase significantly in comparsion with rotational correlation time of protein macromolecule. This result can be explained by macromolecule clusters formation.

Laser light-scattering diagnostic of blood protein solutions

Molecular methods of diagnostics of widespread diseases including vascular pathology on the base static and dynamic laser light scattering in serum blood solution are testified. The alterations of molecular parameters of blood serum of animal species (rats) after experimentally induced cerebral ischemia (hypoxia) and haemorrhagic stroke relative to controls were studied. It was obtained that effective mass of scattering particles in blood serum solutions is diminished for haemorrhagic and ischemic rats in comparison to control. The relative protein concentrations in blood serum also change both after false operation and in case of induced ischemia.

Laser light scattering in diagnostics of widespread diseases

The multiparametric physical method for diagnosing of widespread diseases including oncological disease based on Rayleigh light scattering is proposed. There was studied simultaneously dynamic and static parameters of blood plasma proteins. This method is the product of extensive research conducted on a molecular level on the indicative fields of the human body such as blood serum or lymph. Comprehensive measurements at molecular level and determinations of parameters related to these fluids, especially blood serum, have revealed significant differences in the values of certain parameters in patients with oncological diseases and for healthy individuals.

Optical diagnostics of toxic heavy metal ion's effect on plasma blood proteins

For the first time, the formation of macromolecular protein clusters in water solutions in the presence of heavy metal ions were observed by the light scattering method. Conditions of the formation and destruction of such clusters were investigated. The clusters mass has a maximum value at the isoelectric point of the protein and increases with the ionic strength of the solution. Formation of clusters in the presence of toxic heavy metals in the blood and living cells is believed to have an essential physiological meaning. The molecular mechanisms of clusters formation based on dipole- dipole interactions of the macromolecules are discussed.

Laser investigation of metal ion adsorption on protein charge surface

The behavior of the protein macromolecules in the solution, which can absorb the metal ions on its surface, was considered. The newly found phenomenon consists in anomalies adsorption of heavy metal ions on the protein surface, connecting with a strong binding of its on the protein surface. In this case dipole-dipole interaction became the main interaction force, so when the protein macromolecules approach one to another, a macromolecule cluster can be formed. The light scattering method enables one to calculate the number of absorption centers on the protein surface.

Scattering of laser radiation as a method for diagnosing widespread diseases including oncology

Rayleigh light scattering brings a very rich information about static and dynamic parameters of macromolecules in the solutions including aqueous solutions of proteins. It is known that protein molecule have a big electric charge and great dipole moment connected with it. In our investigation it was shown that optic anisotropy, molecular mass, coefficient of molecular interaction, molecular mobility are varied non-linearly with changing of net charge of macromolecules. On the base of our researches we proposed a simple, affordable optical method of diagnostic of oncological diseases and predisposition.