A. Val del Río

Thermal pre-treatment of aerobic granular sludge: Impact on anaerobic biodegradability

The aerobic granular systems are a good alternative to the conventional activated sludge (AS) ones to reduce the production of sludge generated in wastewater treatment plants (WWTP). Although the quantity of produced sludge is low its post-treatment is still necessary. In the present work the application of the anaerobic digestion combined with a thermal pre-treatment was studied to treat two different aerobic granular biomasses: one from a reactor fed with pig manure (G1) and another from a reactor fed with a synthetic medium to simulate an urban wastewater (G2). The results obtained with the untreated aerobic granular biomasses showed that their anaerobic biodegradability (BD) (33% for G1 and 49% for G2) was similar to that obtained for an activated sludge (30-50%) and demonstrate the feasibility of their anaerobic digestion. The thermal pre-treatment before the anaerobic digestion was proposed as a good option to enhance the BD when this was initially low (33% G1) with an enhancement between 20% at 60 °C and 88% at 170 °C with respect to the untreated sludge. However when the initial BD was higher (49% G2) the thermal pre-treatment produced a slight improvement in the methane production (14% and 18%) and at high temperatures (190 and 210 °C) which did not justify the application of such a treatment.

Aerobic granular SBR systems applied to the treatment of industrial effluents

Four lab scale sequencing batch reactors (SBRs) were operated to remove organic matter and nitrogen from four different industrial wastewaters. The biomass grew in the reactors in the form of aerobic granules characterized by good settling properties. The high biomass concentrations achieved inside the reactors allowed reducing the solids concentration in the effluent down to 0.2 g VSS L(-1). The organic loading rates (OLR) applied to reactors ranged between 0.7 and 5.0 g CODL(-1)d(-1) with removal efficiencies of 60-95%. The nitrogen loading rates (NLR) applied varied between 0.15 and 0.65 g NH(4)(+)-NL(-1)d(-1) with variable removal efficiencies in the four systems (between 15% and 76%).

Greenhouse Gases Emissions from Wastewater Treatment Plants: Minimization, Treatment, and Prevention

The operation of wastewater treatment plants results in direct emissions, from the biological processes, of greenhouse gases (GHG) such as carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O), as well as indirect emissions resulting from energy generation. In this study, three possible ways to reduce these emissions are discussed and analyzed: minimization through the change of operational conditions, treatment of the gaseous streams, and prevention by applying new configurations and processes to remove both organic matter and pollutants. In current WWTPs, to modify the operational conditions of existing units reveals itself as possibly the most economical way to decrease N2O and CO2 emissions without deterioration of effluent quality. Nowadays the treatment of the gaseous streams containing the GHG seems to be a not suitable option due to the high capital costs of systems involved to capture and clean them. The change of WWTP configuration by using microalgae or partial nitritation-Anammox processes to remove ammonia from wastewater, instead of conventional nitrification-denitrification processes, can significantly reduce the GHG emissions and the energy consumed. However, the area required in the case of microalgae systems and the current lack of information about stability of partial nitritation-Anammox processes operating in the main stream of the WWTP are factors to be considered.

Influence of dissolved oxygen concentration on the start-up of the anammox-based process: ELAN®

The anammox-based process ELAN® was started-up in two different sequencing batch reactor (SBR) pilot plant reactors treating municipal anaerobic digester supernatant. The main difference in the operation of both reactors was the dissolved oxygen (DO) concentration in the bulk liquid. SBR-1 was started at a DO value of 0.4 mg O2/L whereas SBR-2 was started at DO values of 3.0 mg O2/L. Despite both reactors working at a nitrogen removal rate of around 0.6 g N/(L d), in SBR-1, granules represented only a small fraction of the total biomass and reached a diameter of 1.1 mm after 7 months of operation, while in SBR-2 the biomass was mainly composed of granules with an average diameter of 3.2 mm after the same operational period. Oxygen microelectrode profiling revealed that granules from SBR-2 where only fully penetrated by oxygen with DO concentrations of 8 mg O2/L while granules from SBR-1 were already oxygen penetrated at DO concentrations of 1 mg O2/L. In this way granules from SBR-2 performed better due to the thick layer of ammonia oxidizing bacteria, which accounted for up to 20% of all the microbial populations, which protected the anammox bacteria from non-suitable liquid media conditions.

Enhanced ammonia removal at room temperature by pH controlled partial nitrification and subsequent anaerobic ammonium oxidation

The Anammox-based processes are suitable for the treatment of wastewaters characterized by a low carbon to nitrogen (C/N) ratio. The application of the Anammox process requires the availability of an effluent with a NO 2−‒ N/NH 4+‒ N ratio composition around 1 g g−1, which involves the necessity of a previous step where the partial nitrification is performed. In this step, the inhibition of the nitrite-oxidizing bacteria (NOB) is crucial. In the present work, a combined partial nitrification-ANaerobic AMmonia OXidation (Anammox) two-units system operated at room temperature (20°C) has been tested for the nitrogen removal of pre-treated pig slurry. To achieve the successful partial nitrification and inhibit the NOB activity, different ammonium/inorganic carbon (NH 4+/IC ) ratios were assayed from 1.19 to 0.82 g NH 4+-N g−1 HCO3‒ C. This procedure provoked a decrease of the pH value to 6.0 to regulate the inhibitory effect over ammonia-oxidizing bacteria caused by free ammonia. Simultaneously, the NOB experienced the inhibitory effect of free nitrous acid which avoided the presence of nitrate in the effluent. The NH 4+/IC ratio which allowed the obtaining of the desired effluent composition (50% of both ammonium and nitrite) was 0.82±0.02 g NH 4+‒ N g−1 HCO 3−‒ C. The Anammox reactor was fed with the effluent of the partial nitrification unit containing a NO 2−‒ N/NH 4+‒ N ratio of 1 g g−1 where a nitrogen loading rate of 0.1 g N L−1 d−1 was efficiently removed.

Bacterial community dynamics in long-term operation of a pilot plant using aerobic granular sludge to treat pig slurry.

Aerobic granular sludge represents an interesting approach for simultaneous organic matter and nitrogen removal in wastewater treatment plants. However, the information about microbial communities in aerobic granular systems dealing with industrial wastewater like pig slurry is limited. Herein, bacterial diversity and dynamics were assessed in a pilot scale plant using aerobic granular sludge for organic matter and nitrogen elimination from swine slurry during more than 300 days. Results indicated that bacterial composition evolved throughout the operational period from flocculent activated sludge, used as inoculum, to mature aerobic granules. Bacterial diversity increased at the beginning of the granulation process and then declined due to the application of transient organic matter and nitrogen loads. The operational conditions of the pilot plant and the degree of granulation determined the microbial community of the aerobic granules. Brachymonas, Zoogloea and Thauera were attributed with structural function as they are able to produce extracellular polymeric substances to maintain the granular structure. Nitrogen removal was justified by partial nitrification (Nitrosomonas) and denitrification (Thauera and Zoogloea), while Comamonas was identified as the main organic matter oxidizing bacteria. Overall, clear links between bacterial dynamics and composition with process performance were found and will help to predict their biological functions in wastewater ecosystems improving the future control of the process.

Effects of the cycle distribution on the performance of SBRs with aerobic granular biomass.

The aerobic granular systems are mainly sequencing batch reactors where the biomass is submitted to feast–famine regimes to promote its aggregation in the form of granules. In these systems, different cycle distributions can be applied for the simultaneous removal of organic matter, nitrogen and phosphorus. In this work two strategies were followed in order to evaluate the effects of the cycle distribution. In the first experiment, the length of the operational cycle was decreased in order to maximize the treatment capacity and consequently the famine/feast ratio was also decreased. In the second experiment, an initial anoxic phase was implemented to improve nitrogen removal efficiency. The results obtained showed that to reduce the famine/feast ratio from 10 to 5 was possible by increasing the treated organic and nitrogen loading rates in the system to 33%, without affecting the removal efficiencies of organic matter (97%) and nitrogen (64%) and producing a slight detriment of the granules characteristics. On the other hand, the implementation of an anoxic phase of 30 min previous to the aerobic one with a pulse-fed mode increased the nitrogen removal of pig manure from 20 to 60%, while the cycle configuration comprising a continuous feeding simultaneous with an anoxic phase of 60 min did not enhance the nitrogen removal and even worsen the ammonia oxidation.

Biomass aggregation influences NaN3 short-term effects on anammox bacteria activity

The main bottleneck to maintain the long-term stability of the partial nitritation-anammox processes, especially those operated at low temperatures and nitrogen concentrations, is the undesirable development of nitrite oxidizing bacteria (NOB). When this occurs, the punctual addition of compounds with the capacity to specifically inhibit NOB without affecting the process efficiency might be of interest. Sodium azide (NaN3) is an already known NOB inhibitor which at low concentrations does not significantly affect the ammonia oxidizing bacteria (AOB) activity. However, studies about its influence on anammox bacteria are unavailable. For this reason, the objective of the present study was to evaluate the effect of NaN3 on the anammox activity. Three different types of anammox biomass were used: granular biomass comprising AOB and anammox bacteria (G1), anammox enriched granules (G2) and previous anammox granules disaggregated (F1). No inhibitory effect of NaN3 was measured on G1 sludge. However, the anammox activity decreased in the case of G2 and F1. Granular biomass activity was less affected (IC50 90 mg/L, G2) than flocculent one (IC50 5 mg/L, F1). Summing up, not only does the granular structure protect the anammox bacteria from the NaN3 inhibitory effect, but also the AOB act as a barrier decreasing the inhibition.

Short- and long-term orange dye effects on ammonium oxidizing and anammox bacteria activities

The effects of orange azo dye over ammonia oxidizing bacteria (AOB) and anammox bacteria activities were tested. Performed batch tests indicated that concentrations lower than 650 mgorange/L stimulated AOB activity, while anammox bacteria activity was inhibited at concentrations higher than 25 mgorange/L. Long-term performance of a continuous stirred tank reactor (CSTR) for the partial nitritation and a sequencing batch reactor (SBR) for the anammox process was tested in the presence of 50 mgorange/L. In the case of the partial nitritation process, both the biomass concentration and the specific AOB activity increased after 50 days of orange azo dye addition. Regarding the anammox process, specific activity decreased down to 58% after 12 days of operation with continuous feeding of 50 mgorange/L. However, the anammox activity was completely recovered only 54 days after stopping the dye addition in the feeding. Once the biomass was saturated the azo dye adsorption onto the biomass was insignificant in the CSTR for the partial nitritation process fed with 50 mgorange/L. However, in the SBR the absorption was determined as 6.4 mgorange/g volatile suspended solids. No biological decolorization was observed in both processes.

Fish-Canning Wastewater Treatment by Means of Aerobic Granular Sludge for C, N and P Removal

This research work analyses the development of aerobic granular sludge to simultaneously remove organic matter (COD), nitrogen (N) and phosphorous (P), from saline fish-canning wastewater. A 1.6 L sequencing batch reactor (SBR) with volumetric exchange ratio (VER) of 50% and a hydraulic retention time (HRT) of 0.25 d, was used. The SBR was operated in 3-hours cycles comprising: 60 min anaerobic feeding, 112 min aeration, 7–1 min settling and 1–7 min effluent discharge. The salt concentration was approximately 10.4 ± 0.8 g NaCl/L, and the applied organic loading rate (OLR) of 5.4 ± 1.9 kg COD/(m3∙d). Under these working conditions, aerobic granules were observed after 34 days of operation, although some filamentous bacteria were present on the surface of the aggregates. The granular biomass had a concentration of volatile suspended solids (VSS) of 1.34 g VSS/L, a mean diameter of 1.35 mm and a density next to 11.5 g VSS/Lgranule. However, after 41 days of operation a fluffy-flocculent suspension was formed, together with granules, probably due to the salinity and the fraction of slowly biodegradable COD of the feeding (≈ 35% of total COD). Good removal efficiencies of soluble COD were observed (≈ 80%), while ammonium and phosphorous were mainly removed to cover the minimum metabolic demand of heterotrophic strains. In fact, the enrichment of the biomass with slow growing autotrophic and phosphorous accumulating bacteria, especially in a saline environment, require a longer time of operation.