Abu Hassan Ahmad

An update on the incidence of dengue gaining strength in Saudi Arabia and current control approaches for its vector mosquito

BackgroundThe cases of dengue reported earlier in the late 1990s from the Kingdom of Saudi Arabia (KSA) occurred in the cities of Jeddah and Makkah. Although the kingdom has ample financial resources to establish effective control measures for the dengue vector, numerous cases of dengue occur and fluctuate in numbers from year to year. This necessitates a serious review of the current vector control strategies being practiced in order to identify the existing shortcomings. This short report provides an update on epidemiology of dengue in KSA (specifically in cities of Jeddah and Makkah) with a critical look at the current vector control strategies.FindingsIn 2013, 4411 cases of dengue were reported, with 8 cases of mortality. This number of dengue incidence was four times higher compared to 2012. In 2013, the highest number of 1272 dengue cases was reported in May, while the lowest number (37) of cases was reported in September.ConclusionsIt is evident that the control strategies of the dengue vector presently employed are inadequate. There seems to be serious deficiencies in following proper scientific procedures during field application(s) of control materials against the vector as is evident by the increases in the number of dengue cases as well as frequent outbreaks of the vector mosquito populations. In this review, some specific suggestions are made to draw attention to the relevant KSA authorities of the possible reasons behind unsuccessful control results and as to how to improve the strategy of dengue vector control in the kingdom.

Morphological Deformities in Chironomus spp. (Diptera: Chironomidae) Larvae as a Tool for Impact Assessment of Anthropogenic and Environmental Stresses on Three Rivers in the Juru River System, Penang, Malaysia

ABSTRACT Morphological deformities in parts of the head capsule of Chironomus spp. larvae inhabiting three polluted rivers (Permatang Rawa [PRR], Pasir [PR], and Kilang Ubi [KUR]) in the Juru River Basin, northeastern peninsular Malaysia, were studied. Samples of the fourth-instar larvae at one location in each river were collected monthly from November 2007 to March 2008 and examined for deformities of the mentum, antenna, mandible, and epipharyngis. At each sample location, in situ measurements of water depth, river width, water pH, dissolved oxygen, and water temperature were made. Samples of river water and benthic sediments were also collected monthly from each larval sample location in each river and taken to the laboratory for appropriate analysis. Total suspended solids (TSSs), ammonium-N, nitrate-N, phosphate-P, chloride, sulfate, and aluminum content in water were analyzed. Total organic matter and nonresidual metals in the sediment samples were also analyzed. Among the three rivers, the highest mean deformity (47.17%) was recorded in larvae collected from KUR that received industrial discharges from surrounding garment and rubber factories, followed by PRR (33.71%) receiving primarily residues of fertilizers and pesticides from adjacent rice fields, and PR (30.34%) contaminated primarily by anthropogenic wastes from the surrounding residential areas. Among the various head capsule structures, deformity of the mentum was strongly reflective of environmental stress and amounted to 27.9, 20.87, and 30.19% in the PRR, PR, and KUR, respectively. Calculated Lenats toxic score index satisfactorily explained the influence of prevailing environmental variables on the severity of mentum deformities. Redundancy analysis and forward selection selected TSSs, sediment Zn, Mn, Cu, and Ni, and water pH, dissolved oxygen, water temperature, total organic matter, nitrate-N, chloride, phosphate-P, ammonium-N, sulfate, and aluminum as parameters that significantly affected some proportion of deformities. The total deformities correlated closely with deformities of mentum but only weakly with deformities in other parts of head. The total deformity incidence was strongly correlated with high contents of sediment Mn and Ni. The mentum and epipharyngis deformities incidence was highly correlated with an increase of TSSs, total aluminum, and ammonium-N and a decrease in pH and dissolved oxygen.

First records of two species of Oriental scuttle flies (Diptera: Phoridae) from forensic cases

In Penang, Malaysia, the Oriental and Afrotropical Megaselia curtineura (Brues) and the Oriental and Japanese Megaselia spiracularis Schmitz are reported from human corpses, these being the first reports of these species in such forensic cases.

Genotoxicity of heavy metals to the larvae of Chironomus kiiensis Tokunaga after short-term exposure.

The genotoxic effects of increasing concentrations (below lethal concentration [LC50]) of cadmium ([Cd] 0.1, 1 and 10 mg/L), copper ([Cu] 0.2, 2 and 20 mg/L) and zinc ([Zn] 0.5, 5 and 50 mg/L) on Chironomus kiiensis were evaluated using alkaline comet assay after exposure for 24 h. Both the tail moment and the olive tail moment showed significant differences between the control and different concentrations of Cd, Cu and Zn (Kruskal–Wallis, p < 0.05). The highest concentration of Cd was associated with higher DNA damage to C. kiiensis larvae compared with Cu and Zn. The potential genotoxicity of these metals to C. kiiensis was Cd > Cu > Zn.

Colonized Aedes albopictus and its sexual performance in the wild: implications for SIT technology and containment

BackgroundMating is a physiological process of crucial importance underlying the size and maintenance of mosquito populations. In sterile and incompatible insect technologies (SIT and IIT), mating is essential for mass production, persistence, and success of released individuals, and is a central parameter for judging the effectiveness of SIT/IIT programs. Some mosquitoes have an enormous reproductive potential for both themselves and pathogens and mating may contribute to persistence of infection in nature. As Aedes albopictus can transmit flaviviruses both sexually and horizontally, and as infected insects are usually derived from laboratory colonies, we investigated the implications of mating between a long-term laboratory colony of Ae. albopictus and wild populations.MethodsThrough a series of mating experiments, we examined the reproductive outcomes of sexual cross-affinity between laboratory-raised and wild adults of Ae. albopictus.ResultsThe results indicated appreciable mating compatibility between laboratory-reared and wild adults, and equivalent levels of egg production among reciprocal crosses. We also observed comparable larval eclosion in lab females mated with wild males, and increased adult longevity in female offspring from wild females|×|laboratory males crosses.ConclusionsTaken together, these data suggest that Ae. albopictus can preserve its reproductive fitness over a long period of time in the laboratory environment and has valuable attributes for SIT application. These observations together with the ability to successfully inseminate heterospecific females indicate the potential of Ae. albopictus to act as an ecological barrier if non-sterilized males are massively released in areas occupied by Aedes aegypti. The observed substantial reproductive fitness combined with the capability to reproduce both, itself and viruses illustrates the potential of Ae. albopictus to pose a serious threat if infected and released accidentally.

Coupling of remote sensing data and environmental-related parameters for dengue transmission risk assessment in Subang Jaya, Malaysia

In Malaysia, the endemic level of dengue fever (DF) has already changed morbidity indicators, and the magnitude of these incidences in the last few years has surpassed the incidences of all other diseases of compulsory notification. The reasons for the dramatic emergence of DF are complex and not well understood. There are many factors that contribute to the epidemiological conditions that favour viral transmission by the main mosquito vector. This study, therefore, is filling this gap by analysing the impact of dengue incidence at a local (Subang Jaya) scale using environmental factors. Meteorological data and land-use pattern were consolidated using geographic information system (GIS) and its components as an analytical tool. We have shown that weather variables (relative humidity, temperature and precipitation) have significant correlation with DF incidence with seasonal variation. Besides land-use pattern, DF incidence shows the higher distribution in the residential area, followed by commercial and industrial area. This is due to the higher population density in residential area as well as favourable places for the breeding of dengue-carrying Aedes mosquitos created by humans in the residential area, especially one-storey houses. The analysis on the trends of DF incidence towards various housing types indicate that most of the victims’ houses fall into interconnection houses and mixed houses types compared to the independent houses area. The outcome driven from this analysis suggested that each character of the environmental factors has their own risk towards dengue incidence. In line with that, it is possible to develop a dynamic model of DF transmission using the knowledge produced by this comprehensive time series data and the results provided by the different analyses.

Biodiversity of stream insects in the Malaysian Peninsula: spatial patterns and environmental constraints

The spatial variation in Ephemeroptera, Plecoptera, and Trichoptera (EPT) taxonomic composition in headwater forested streams of peninsular Malaysia was studied. Several environmental parameters were assessed for their importance in regulating the composition of assemblages across the spatial extent of the Malaysian bioregion. The spatial variables were expressed as vectors based on principal coordinates of neighbour matrices (PCNM). The forward selection based on two stopping criteria was used to identify the key environmental and spatial variables controlling the EPT taxonomic composition. Eigenvector‐based spatial filtering was employed to investigate spatial patterns in EPT composition, and the variation partitioning technique was used to determine the relative importance of environmental and spatial factors in application on EPT composition. The forward selection retained five parameters [altitude, biochemical oxygen demand (BOD), water velocity, ammonia, and dissolved oxygen (DO)] which explained 13.2% of the total variance in EPT composition. The three selected spatial variables (PCNM vectors) accounted for 8.4% of the composition variation. However, the spatially‐structured environmental variables explained 7.5% of the total variance in EPT composition. The variation in altitude correlated with broad‐scale spatial extent and was the most important factor regulating the EPT composition in peninsular Malaysia. Our study revealed spatially‐structured patterns in the EPT composition, showing a richness decrease from the south to the north of peninsular Malaysia.

Household survey of container-breeding mosquitoes and climatic factors influencing the prevalence of Aedes aegypti (Diptera: Culicidae) in Makkah City, Saudi Arabia

OBJECTIVE To investigate the prevalence of container breeding mosquitoes with emphasis on the seasonality and larval habitats of Aedes aegypti (Ae. aegypti) in Makkah City, adjoining an environmental monitoring and dengue incidence. METHODS Monthly visits were performed between April 2008 and March 2009 to randomly selected houses. During each visit, mosquito larvae were collected from indoors and outdoors containers by either dipping or pipetting. Mosquitoes were morphologically identified. Data on temperature, relative humidity, rain/precipitations during the survey period was retrieved from governmental sources and analyzed. RESULTS The city was warmer in dry season (DS) than wet season (WS). No rain occurred at all during DS and even precipitations did fall, wetting events were much greater during WS. Larval survey revealed the co-breeding of Aedes, Culex and Anopheles in a variety of artificial containers in and around homes. 32 109 larvae representing 1st , 2nd, 3rd, and 4th stages were collected from 22 618 container habitats. Culicines was far the commonest and Aedes genus was as numerous as the Culex population. Ae. aegypti larval abundance exhibited marked temporal variations, overall, being usually more abundant during WS. Ten types of artificial containers were found with developing larvae. 70% of these habitats were located indoors. 71.42% of indoor containers were permanent and 28.58% was semi-permanent during WS. Cement tanks was the only container type permanent during DS. Ae. aegypti larval indices (CI, HI, BI) recorded were greater during WS. CONCLUSIONS Taken together, these results indicate a high risk of dengue transmission in the holy city.

Unusual developing sites of dengue vectors and potential epidemiological implications

OBJECTIVE To identify the unusual breeding sites of two dengue vectors, i.e. Aedes albopictus (Ae. albopictus) and Aedes aegypti (Ae. aegypti). METHODS During the second half of 2010, we performed an occasional survey in rural (Teluk Tempoyak) and urban (Gelugor) areas of Penang Island, Malaysia, to identify cryptic breeding sites. RESULTS In the rural area, we found heterogeneous immature stages of Ae. albopictus in the water bowl of an encaged bird. We also observed Ae. aegypti eggs deposited in the flush tank of a toilet in the urban area. CONCLUSIONS It can be concluded that both breeding patterns can increase contact with hosts (humans and birds) and presumably population densities of Ae. albopictus and Ae. aegypti, thereby potentially boosting the risks for spread and transmission of arboviral diseases.

The biology and demographic parameters of Aedes albopictus in northern peninsular Malaysia

OBJECTIVE To generate life table characteristics for the dengue vector Aedes albopictus (A. albopictus) under uncontrolled conditions, incorporating both the aquatic and the adult stages. METHODS Ten females derived from wild pupae were allowed to fully blood-feed on restrained mice. 774 eggs were hatched in seasoned water. F1 larvae were followed for development until their F2 counterparts emerged as adults. Some population parameters were monitored (F1) or estimated (F2). RESULTS A. albopictus exhibited increased fecundity and egg hatch success. Immature development was quick. Immature survival was high, with lowest rate in the pupal stage. Adult emergence was about 81% and sex ratio was close to 1:1. Generational mortality (K) was about 28%. A high proportion of females completed a reproductive cycle and the obtained parity rate was predicted to lead to higher fecundity in the next generation. CONCLUSIONS It can be concluded that natural A. albopictus populations in Penang seem largely determined by quick development in combination with low immature loss and increased oviposition.