Adrien Plecis
Centre national de la recherche scientifique
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Publication
Featured researches published by Adrien Plecis.
Analytical Chemistry | 2008
Adrien Plecis; Clément Nanteuil; Anne-Marie Haghiri-Gosnet; Yong Chen
We report on the systematic investigation of electropreconcentration phenomena in hybrid micro/nanofluidic devices. The competition between the electroosmotic dragging force and the highly nonlinear electrophoretic forces induced by the polarization effect is responsible for four preconcentration regimes within such structures that can arise at both cathodic and anodic sides of the nanochannel. Numerical calculations on the spatiotemporal concentration of charged molecules confirm such a classification, showing a general agreement with the reported experimental data at low and moderate ionic strengths. The results also suggest that both the mobility and the valence of the species of interest are important parameters in the determination of the preconcentration rates.
Analytical Chemistry | 2013
Anne-Claire Louër; Adrien Plecis; Antoine Pallandre; Jean-Christophe Galas; André Estevez-Torres; Anne-Marie Haghiri-Gosnet
We investigate the preconcentration profiles of a fluorescein and bovine serum albumin derivatized with this fluorescent tag in a microfluidic chip bearing a nanoslit. A new preconcentration method in which a hydrodynamic pressure is added to both electroosmotic and electrophoretic contributions is proposed to monitor the location of the preconcentration frontline. A simple predictive model of this pressure-assisted electropreconcentration is proposed for the evolution of the flow profile along this micro/nano/microfluidic structure. We show with a small analyte such as fluorescein that the additional hydrostatic pressure mode enables to stabilize the concentration polarization (CP) effect, resulting in better control of the cathodic focusing (CF) peak. For BSA (bovine serum albumin), we exhibit that the variation of the hydrodynamic pressure can have an even more drastic effect on the preconcentration. We show that, depending on this hydrodynamic pressure, the preconcentration can be chosen, either in the cathodic side or in the anodic one. For the first time, we prove here that both anodic focusing (AF) and cathodic focusing (CF) regimes can be reached in the same structures. These results also open new routes for the detection and the quantification of low abundance biomarkers.
Analytical Chemistry | 2008
Adrien Plecis; Yong Chen
A microfluidic analogy of the electric Wheatstone Bridge has been developed for electrokinetic study of miscellaneous liquid-solid interfaces. By using an optimized glass-PDMS-glass device technology, microfluidic channels with well-controlled surface properties can be fabricated, forming an H shaped fluidic network. After solving a set of linear equations, the electro-osmotic flow rate in the center channel can be deduced from indirect measurement of flow rates in the lateral channels. Experimentally, we demonstrate that the electro-osmotic mobility can be monitored every 30 s with accuracy better than 3% for a large dynamic range of electric fields. The results obtained with a borosilicate glass (D-263) and several standard biological buffers are also shown to illustrate the capability of this high throughput method.
Journal of Applied Physics | 2008
Adrien Plecis; Laurent Malaquin; Yong Chen
Fast monitoring of flow rates in microchannels is needed for a variety of microfluidic investigations. We propose a simplified approach of conventional particle image velocimetry by using a full-frame correlation function based image algorithm. Experimentally, flow rates in microfluidic channels could be determined in a few seconds for a maximum flow velocity ranging from 10u2002μmu2009s−1 to 1u2002mmu2009s−1 with a standard deviation of less than 4%. The processing parameters have also been studied both experimentally and theoretically, in order to evaluate the validation range of the proposed method.
Langmuir | 2015
Antoine Pallandre; Sonia Korchane; Isabelle Le Potier; Jean Gamby; Benjamin Lassus; Sébastien Méance; Syrine Chebil; Adrien Plecis; Bo Xiong; Catherine Ringard-Lefebvre; Véronique Rosilio; Myriam Taverna; Anne-Marie Haghiri-Gosnet
This paper describes the measurement of the electroosmotic mobility (EOF) in a Wheatstone fluidic bridge (μFWB) as a direct probe of the surface instability. The variation of EOF known as one major contribution of the electrokinetic migration has been determined with a real-time measurement platform after different conditionings on chips. We also scan the pH of the background electrolytes with three different ionic strengths to evaluate the dependencies of the EOF as a function of the pH. A hysteresis methodology has been developed for probing the surface charge instabilities. EOF mobility has been recorded during on-a-chip electrophoresis to estimate the effect of such instability on the analytical performance. As expected, our experimental curves show that a decrease in the ionic strength increases the surface charge stability of the hybrid microchip. This result demonstrates that ionic exchanges between the surface and the fluid are clearly involved in the stability of the surface charge. With this original method based on real-time EOF measurement, the surface state can be characterized after hydrodynamic and electrophoresis sequences to mimic any liquid conditioning and separation steps. Finally, as a demonstrative application, isotherms of the adsorption of insulin have been recorded showing the change in surface charge by unspecific adsorption of this biomolecule onto the microfluidic channels wall. These methodologies and findings could be particularly relevant to investigating various analytical pathways and to understanding the molecular mechanisms at solid/liquid interfaces.
Microelectronic Engineering | 2007
Adrien Plecis; Yong Chen
Lab on a Chip | 2011
Guilhem Velvé Casquillas; Chuanhai Fu; Maël Le Berre; Jeremy Cramer; Sébastien Méance; Adrien Plecis; Damien Baigl; Jean-Jacques Greffet; Yong Chen; Matthieu Piel; Phong T. Tran
Microelectronic Engineering | 2008
Adrien Plecis; Yong Chen
Lab on a Chip | 2010
Adrien Plecis; J. Tazid; Antoine Pallandre; P. Martinhon; C. Deslouis; Yong Chen; Anne-Marie Haghiri-Gosnet
Lab on a Chip | 2011
Adrien Plecis; Antoine Pallandre; Anne-Marie Haghiri-Gosnet