Ahmad B. Cader

Chlamydia Pneumoniae-Infected Astrocytes Alter Their Expression of ADAM10, BACE1, and Presenilin-1 Proteases

brain. To investigate this, we are characterising the effects of CD2AP knockdown in an in vitro model of the BBB. Methods: Endogenous CD2AP expression was knocked down in H4 and hMEC/D3 cells using siRNA. Knockdown was quantified by western blot and Image J analysis. Levels of APP, Ab, and APP metabolites were quantified using ELISAs. The rate of receptor-mediated endocytosis was measured by a transferrin uptake assay. Co-localisation of CD2AP with APP and endosomal markers was assessed using immunocytochemistry. Transcytosis in hMEC/D3 cells will be assessed using Transwell assays. Results:We observed a significant 30.2760.07 % increase in Ab40 expression (p1⁄40.0046). Expression levels of APP and APP processing metabolites was not significantly altered. Results from our experiments in progress will be made available at AAIC 2016. Conclusions:Knockdown of CD2AP expression resulted in a significant increase in extracellular amyloid-B (Ab) levels, which did not appear to be directly related to changes in APP processing. This study is ongoing and novel data will be presented at AAIC 2016. Overall, our study provides valuable insights into how CD2AP contributes to LOAD susceptibility.