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Dive into the research topics where Ahmad Seyed-Mozaffari is active.

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Featured researches published by Ahmad Seyed-Mozaffari.


Biochemical Pharmacology | 1995

Identification and quantification of the indole alkaloid ibogaine in biological samples by gas chromatography-mass spectrometry

Carol A. Gallagher; Lindsay B. Hough; Sandra M. Keefner; Ahmad Seyed-Mozaffari; Sydney Archer; Stanley D. Click

A sensitive and highly selective analytical chemical method for measuring the indole alkaloid ibogaine in biological samples has been developed. The method utilizes organic extraction, derivatization with trifluoroacetic anhydride, and detection by combined gas chromatography-mass spectrometry. The deuterated analog of ibogaine, O-[Cd3]-ibogaine, was synthesized and used as an internal standard for the method. Standard curves, constructed from variable amounts of ibogaine (50-400 ng) and a fixed amount of internal standard (250 ng) were linear. The method has an approximate detection limit of at least 20 ng/mL of tissue extract (180 ng/g tissue), with a coefficient of variation of 8 to 12.5%. Chemical stability studies with the method found that aqueous ibogaine solutions (1-10 mg/mL) could be stored at 10 degrees for up to 7 months with no more than 10% loss. The method was also used to measure brain ibogaine levels in rats 1 and 19 hr after a single dose of drug (40 mg/kg, i.p.); the results suggest a rapid disappearance of the drug after i.p. dosing. The method will help reveal the pharmacokinetic properties of this putative anti-addictive agent in animals and humans.


Molecular and Biochemical Parasitology | 1990

Mode of action of the schistosomicide Hycanthone: site of DNA alkylation

Sydney Archer; Wageeh El-Hamouly; Ahmad Seyed-Mozaffari; Richard H. Butler; Livia Pica-Mattoccia; Donato Cioli

Condensation of hycanthone N-methylcarbamate (HNMC) with deoxyguanosine (dG) furnished a mixture of the N-1 and N2 adducts which were purified and characterized as their acetates. Condensation of HNMC with thymidine (T) gave the N-3 adduct in poor yield. Adenosine (A) and cytidine (C) did not react with HNMC. Incubation of schistosomes with either [3H]hycanthone (HC) or [3H]HNMC furnished DNA to which [3H]HC was covalently bound. The alkylated DNA was degraded enzymically and the radiolabeled nucleosides were separated using HPLC. Two major peaks were observed which coincided in retention time with the synthetic N-1 and N2 alkylated dG. Alkylated T was absent. Thus, the site of alkylation of DNA by either HC or HNMC is dG.


Journal of Immunological Methods | 1997

Fluorescent staining of κ opioid receptors using naltrexamine derivatives and phycoerythrin

Diane M.P. Lawrence; Ian Hutchinson; Ahmad Seyed-Mozaffari; Sydney Archer; Jean M. Bidlack

An immunofluorescent technique that is more sensitive than radioligand binding was developed in order to detect opioid receptors expressed on leukocytes. The current study was designed to optimize the method for fluorescently labeling kappa opioid receptors. For these experiments, the opioid antagonist naltrexamine was conjugated to either fluorescein (FITC-NTXamine) or biotin (biotin-NTXamine). One-step, two-step, and three-step protocols were compared to determine which procedure resulted in optimal staining of the kappa opioid receptor expressed on intact, unfixed R1E/TL8x.1.OUAr.1(R1EGO) cells, a thymoma known to express kappa opioid receptors. The one-step method involved incubating cells with FITC-NTXamine, and the fluorescein intensity was measured by flow cytometry. In the two-step method, cells were incubated with biotin-NTXamine, followed by extravidin-conjugated phycoerythrin, and the phycoerythrin fluorescence was measured. Finally, in the three-step protocol, cells were incubated with FITC-NTXamine, followed by biotin-conjugated anti-fluorescein IgG, then extravidin-phycoerythrin. The one-step protocol stained the cells, but the signal was not diminished in the presence of opioid competitors. The two-step approach did not stain cells significantly above background levels. Only the three-step approach yielded staining that was displaced by the kappa-selective antagonist nor-binaltorphimine. Thus, the addition of a secondary biotinylated antibody, resulting in the amplification of binding, which was detected using phycoerythrin as a fluorophore, was required to detect low levels of opioid receptor expression on leukocytes.


Biochemical Pharmacology | 1992

Synthesis and characterization of 7-nitrobenzo-2-oxa-1,3-diazole (NBD)-labeled fluorescent opioids.

Sydney Archer; Fedor Medzihradsky; Ahmad Seyed-Mozaffari; Paul J. Emmerson

Alkylation of sarcosine with 4-chloro-nitrobenzo-2-oxa-1,3-diazole (NBD-chloride) furnished a fluorescent tag that was coupled with a tetrahydrothebaine derivative and beta-naltrexamine, respectively, to yield the fluorescent opioids 7 alpha-(1R)-1-hydroxy-1-methyl-3-(4-hydroxyphenyl)-propyl]-6,14- endoethenotetrahydrothebaine NBD-sarcosinate (ASM-5-10) and N-cyclopropylmethyl-3-hydroxy-14 beta-hydroxy-6 beta-(NBD sarcosinyl)-amino-epoxymorphinan (ASM-5-67). The fluorescence intensity of the novel opioids allowed their detection at subnanomolar concentrations, and was dependent on the polarity of the solvent. Maximum quantum yield was obtained in ethyl acetate and ethanol, and minimal fluorescence in heptane and water. Compounds ASM-5-10 and ASM-5-67 displaced the opioid receptor binding of [3H]Tyr-D-Ala-Gly-(Me)Phe-Gly-ol in monkey brain membranes with IC50 values of 8.4 and 1.5 nM, respectively. Whereas ASM-5-67 bound to mu, delta, and kappa receptors with comparable affinities, ASM-5-10 was mu-selective, with selectivity indices (ratio of respective IC50 values) of 0.04 for both mu/delta and mu/kappa. The sodium response ratio in binding revealed a pronounced agonist property of ASM-5-10. Both opioids were lipophilic, with octanol-water partition coefficients (log Papp) of 2.8 (ASM-5-10) and 1.0 (ASM-5-67). ASM-5-10 exhibited particularly strong membrane retention that was not reversible by four washes. Their favorable characteristics in fluorescence, receptor binding, and membrane interaction make these newly developed ligands useful molecular probes to study opioid receptor mechanisms.


Life Sciences | 1993

Photoaffinity ligands for the MU opioid receptor

Eric J. Simon; Li-Qun Fan; Jacob M. Hiller; Ahmad Seyed-Mozaffari; Arthur G. Schultz; Sydney Archer

Two affinity ligands, 6 beta-(5-Azido-2-nitrophenacetamido) 14 beta-hydroxy-7,8-dihydromorphinone (4) and 6 beta-(5-azido-2-nitrophenacetamido) 14 beta-hydroxy-7,8-dihydro-N- cyclopropylmethylnormorphinone (5) bind reversibly to opioid receptors present in bovine caudate membranes and photolyse in a range of wavelengths centered about 366 nm to produce wash-resistant binding to the mu receptor. At these wavelengths very little if any photodestruction of the mu receptor occurs over the 20 minute period of irradiation at 0 degree C.


Bioorganic & Medicinal Chemistry Letters | 1995

N-Methyl and N-cyclopropylmethyl-14α,14′β-[dithiobis[(2-oxo-2,1-ethanediyl)imino]]bis(7,8-dihydro-5β-methyl-morphinone) MET-TAMO and N-CPM-MET-TAMO: Synthesis and opioid binding properties

Jean M. Bidlack; Richard A. Kaplan; Alice Sebastian; Ahmad Seyed-Mozaffari; Ian Hutchinson; Sydney Archer

Abstract MET-TAMO and N-CPM-MET-TAMO were prepared by the same procedure used for the corresponding 5-desmethyl compounds, TAMO and N-CPM-TAMO, except that a new procedure was employed to synthesize the intermediate, 14β-amino-7,8-dihydromorphinone. Both MET-TAMO and N-CPM-MET-TAMO produced wash-resistant inhibition of μ, δ and κ binding but were more potent at the μ receptor.


European Journal of Medicinal Chemistry | 1989

Opioid ligands related to tifluadom

Sydney Archer; Ahmad Seyed-Mozaffari; Eric J. Simon; Theresa L. Gioannini

In an effort to prepare ligands with kappa selective affinity suitable for use in affinity chromatography, we synthesized the haloacetamido derivatives 17 and 18 of tifluadom. These compounds showed modest opioid binding affinity but were more active at the mu than at the kappa site. The nitro compounds 7 and 16 were prepared as potential intermediates. The former, a weak mu agonist, was devoid of kappa affinity (IC50 > 10−6 M), whereas the latter was a mu selective agonist.Abstract In an effort to prepare ligands with kappa selective affinity suitable for use in affinity chromatography, we synthesized the haloacetamido derivatives 17 and 18 of tifluadom. These compounds showed modest opioid binding affinity but were more active at the mu than at the kappa site. The nitro compounds 7 and 16 were prepared as potential intermediates. The former, a weak mu agonist, was devoid of kappa affinity (IC 50 > 10 −6 M), whereas the latter was a mu selective agonist.


European Journal of Medicinal Chemistry | 1989

Original paperOpioid ligands related to tifluadomLigands opioïdes apparentés au tifluadom

Sydney Archer; Ahmad Seyed-Mozaffari; Eric J. Simon; Theresa L. Gioannini

In an effort to prepare ligands with kappa selective affinity suitable for use in affinity chromatography, we synthesized the haloacetamido derivatives 17 and 18 of tifluadom. These compounds showed modest opioid binding affinity but were more active at the mu than at the kappa site. The nitro compounds 7 and 16 were prepared as potential intermediates. The former, a weak mu agonist, was devoid of kappa affinity (IC50 > 10−6 M), whereas the latter was a mu selective agonist.Abstract In an effort to prepare ligands with kappa selective affinity suitable for use in affinity chromatography, we synthesized the haloacetamido derivatives 17 and 18 of tifluadom. These compounds showed modest opioid binding affinity but were more active at the mu than at the kappa site. The nitro compounds 7 and 16 were prepared as potential intermediates. The former, a weak mu agonist, was devoid of kappa affinity (IC 50 > 10 −6 M), whereas the latter was a mu selective agonist.


European Journal of Pharmacology | 1993

Antinociceptive evaluation of 14β-(bromoacetamido)-7,8-dihydro-N(cyclopropylmethyl)-normorphinone in mice

Qi Jiang; Ahmad Seyed-Mozaffari; Sydney Archer; Jean M. Bidlack

This study evaluated the supraspinal opioid effects of 14 beta-(bromoacetamido)-7,8-dihydro-N(cyclopropylmethyl)-normorphinone+ ++ (N-CPM-H2BAMO) in the mouse acetic acid-induced writhing and tail-flick assays. In the writhing test, N-CPM-H2BAMO produced a time- and dose-dependent antinociception after i.c.v. administration, with a 50% antinociceptive response being obtained with 0.28 (0.19-0.39) nmol when given 10 min before testing. The antinociceptive effect of N-CPM-H2BAMO was antagonized in a dose-dependent manner by the kappa-selective opioid receptor antagonist, nor-binaltorphimine. In the mouse tail-flick assay, N-CPM-H2BAMO failed to produce any antinociception after i.c.v. administration. N-CPM-H2BAMO produced a dose-dependent antagonism of morphine-induced antinociception but not antinociception induced by the delta-opioid receptor agonist [D-Pen2,D-Pen5]enkephalin. Nor-binaltorphimine (0.3 nmol) at dose that completely antagonized N-CPM-H2BAMO-induced antinociception in the writhing assay did not prevent the antagonistic effect of N-CPM-H2BAMO on morphine-induced antinociception. Therefore, these data indicate that N-CPM-H2BAMO produces antinociception by acting at supraspinal kappa-opioid receptors in the writhing assay, and also acts as a mu-opioid receptor antagonist.


Journal of Medicinal Chemistry | 1988

Preparation and antischistosomal and antitumor activity of hycanthone and some of its congeners. Evidence for the mode of action of hycanthone.

Sydney Archer; Livia Pica-Mattoccia; Donato Cioli; Ahmad Seyed-Mozaffari; Abdel Hadi Zayed

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Sydney Archer

Rensselaer Polytechnic Institute

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Qi Jiang

University of Rochester

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Alice Sebastian

Rensselaer Polytechnic Institute

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Ian Hutchinson

Rensselaer Polytechnic Institute

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Livia Pica-Mattoccia

Rensselaer Polytechnic Institute

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Donato Cioli

National Research Council

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