Ahmed Nassar

Camel (Camelus dromedarius) and sheep (Ovis aries) meat as a source of dog infection with some coccidian parasites

Experimental infection of dogs with camel (Camelus dromedarius) meat resulted in infection of the dogs with Isospora canis, Hammondia heydorni and Sarcocystis cameli. The dogs fed sheep (Ovis aries) meat passed oocysts of Isospora canis, Isospora ohioensis and sporocyts of Sarcocystis spp. Extraintestinal stages were detected in the intestinal lymph node of a rabbit killed 4 days following inoculation with Isospora ohioensis oocysts. Dogs fed the rabbit (killed 4 days after inoculation with I. ohioensis) passed I. ohioensis oocysts in their faeces 8 days post-infection.

OBSERVATIONS ON THE USE OF ELISA FOR DETECTION OF BABESIA BIGEMINA SPECIFIC ANTIBODIES

An indirect enzyme-linked immunosorbent assay (ELISA) was evaluated to study the cause of the high level of background reactions which hinders the application of ELISA as a field diagnostic test for Babesia bigemina. Different blockers to improve the specificity of the ELISA were compared. THe use of soya milk (25%), gelatin (2.5%) and chicken serum (2%) did not significantly improve the specificity of the test. It was noted that the presence of fibrinogen contributed to the positive ELISA results more than the presence of B. bigemina specific antigen. This conclusion was confirmed by testing bovine fibrinogen as a host protein antigen in ELISA which strongly responded against B. bigemina positive control sera. It is suggested that application of ELISA for B. bigemina is still unreliable until a more purified Babesia-specific antigen or specific monoclonal antibodies are available.

Haematological and biochemical changes in buffalo calves inoculated with Sarcocystis fusiformis from cats

Two groups of buffalo calves were infected with Sarcocystis fusiformis sporocysts. Animals of the first group received each 5 X 10(5) sporocysts, those of the second group 5 million sporocysts. All calves were clinically normal during 6 weeks after infection. Minor changes were observed in the blood cytology, serum alkaline phosphatase, glutamic oxalacetic transaminase, glutamic pyruvic transaminase, total proteins, urea and glucose of infected buffalo calves.

Eosinophilic granuloma of the temporal bone in children.

AbstractEosinophilic granuloma (EG) is a bony destructive disease that frequently occurs in children; it is a subtype of Langerhans cell histiocytosis. The aims of this study were to detect the presenting features of temporal bone lesions in children and to evaluate the efficacy of surgery combined with radiotherapy in treatment of the disease. A retrospective study on 12 children with EG of the temporal bone was done. Computed tomography and hearing assessment were performed for all patients. All patients were treated with cortical mastoidectomy followed by postoperative radiotherapy. Follow-up was carried out for at least 2 years. The patients’ presenting symptoms were external ear canal mass in 10 patients (83.3%), postauricular swelling in 8 patients (66.7%), and persistent otorrhea in 4 patients (33.3%). Ten patients (83.3%) showed conductive hearing loss, whereas 2 patients (16.7%) showed mixed hearing loss on the affected side. Computed tomography showed osteolytic defects without sclerotic margins filled with soft tissue masses involving the mastoid bone. Histopathologic examination showed eosinophils and Langerhans cells that were immune reactive for CD1 antigen and S-100 protein. Postoperative follow-up showed complete cure of the disease in 10 children (83.3%), with recurrence detected in 2 patients (16.7%) who needed second surgical intervention. We concluded that temporal bone EG in children may present with features that mimic the features of chronic suppurative otitis media. However, computed tomography and histopathologic examination are diagnostic. Cortical mastoidectomy together with postoperative radiotherapy is an achievable treatment in most cases.

Ultrastructure of Sarcocystis spp. from donkeys (Equus asinus) in Egypt

The fine structure of Sarcocystis spp. from donkeys (Equus asinus) in Egypt is described. Sarcocysts were found in the oesophagus, diaphragm and heart of 18 of 20 donkeys. Only one type of mature muscle cyst was found. Sarcocysts were 120-410.6 X 48.4-50.2 microns. The primary cyst wall had numerous 3.3-3.7 microns villi. Each villus contained 20-60 fibrillar elements which extended from the ends and sides of the villi throughout the ground substance, where they became tightly packed. The bundles of fibrillar elements formed junctions with the pellicles of the metrocytes. Ultrastructurally, Sarcocystis spp. of the donkey was similar to sarcocysts previously described from the horse.

Mucocele of the hard palate in children

OBJECTIVE Mucus retention cyst of the hard palate may result from obstruction of the ducts of the minor salivary glands, and it was defined as a mucocele. Although, the disease is not common in the hard palate, it was previously reported by many authors in the soft palate. The aim of our study was to present pediatric patients who were diagnosed to have mucocele of the hard palate, and to evaluate the outcome of the surgical excision of this lesion. METHODS This is a case series study included 8 pediatric patients who presented with cystic lesions on the hard palate which were removed surgically, and were diagnosed as mucoceles. Preoperative data, surgical procedures, and postoperative outcome were presented. Follow up of patients was performed for at least one year. RESULTS The swelling was detected as a single isolated lesion, on the side of the hard palate, covered with healthy mucosa, not tender, oval or round in shape, and measuring 0.4 to 1.7cm in its greatest dimension. Computed tomography showed a well defined cavity which was not invading the bone, and not disrupting the muscles of the palate. Histopathological examination confirmed that the lesion was a cavity that is lined with an epithelial layer with pseudoepitheliomatous hyperplasia. No patients developed intraoperative or postoperative complications, and no recurrence was detected in any patient. CONCLUSIONS Oral mucoceles can develop on the hard palate of the children, the lesions are mucus retention cysts. Complete surgical removal of the lesions with their cystic wall is a good treatment options, it carries no risk of recurrence.

Furlow palatoplasty for previously repaired cleft palate with velopharyngeal insufficiency.

OBJECTIVE Velopharyngeal insufficiency (VPI) is a common complication after cleft palate repair, it may be due to lack of levator sling reconstruction and/or palatal shortening. Furlow palatoplasty has the advantages of retro-positioning of levator palati muscles and palatal lengthening. The aim of this study was to assess the efficacy of Furlow palatoplasty in the treatment of VPI in patients who undergone previous palatoplasty. METHODS Twenty-three children with post-palatoplasty VPI were included in the study. Furlow technique which was not used in the primary repair, has been used as a secondary corrective surgery. Preoperative and postoperative evaluation of velopharyngeal function was performed, using auditory perceptual assessment (APA) and nasometry for speech, and flexible nasopharyngoscopy for velopharyngeal closure. RESULTS Significant improvement of APA and nasalance score for oral and nasal sentences was achieved. Flexible nasopharyngoscopy showed complete velopharyngeal closure in 19 patients (82%) postoperatively. CONCLUSION Furlow palatoplasty is considered a useful treatment option for VPI in patients with previously repaired cleft palate, it improves the speech and velopharyngeal closure.

Multichannel Clock and Data Recovery: A Synchronous Approach

This brief proposes a scalable multichannel clock and data recovery architecture that exploits the synchrony of multiple point-to-point serial links and uses a single voltage-controlled oscillator (VCO) to drive multiple phase detection loops. The proposed architecture can be naturally reduced by design to an ensemble of weakly interacting delay-locked loops. As a result, the jitter peaking problem is asymptotically eliminated, which makes this architecture well suited for use in long-haul repeater chains. Moreover, it allows controlling VCO jitter transfer to the recovered clock without affecting data jitter transfer. The architecture is demonstrated both by a Verilog-A behavioral model along with a rigorous system and statistical analysis.

Effectiveness of modified cautery-assisted palatal stiffening operation in controlling snoring

Objective The aim of the study was to assess the effectiveness of modified cautery-assisted palatal stiffening operation (CAPSO) to treat snoring. Patients and methods Fifty patients with simple snoring underwent the modified CAPSO; patients underwent polysomnography, preoperatively and at 3 months postoperatively. Results All patients had improvement in their snoring, and the patients and their sleep partners were satisfied with the result at 3 months postoperatively. Conclusion The modified CAPSO technique has shown promising and encouraging results in a small cohort of patients with simple snoring.