Ahmet C. Caliskan

Effect of Peroxisome Proliferator—Activated Receptor-γ Agonist Rosiglitazone on the Induction of Endometriosis in an Experimental Rat Model

Objective: To assess the effect of the peroxisome proliferator-activated receptor (PPAR)-γ agonist rosigliotazone on the induction of endometriosis in a rat model. Methods: Endometriosis was surgically induced in 28 rats by transplanting an autologous fragment of endometrial tissue onto the inner surface of the abdominal wall. Group I was assigned as control and no medication was administered. Starting 3 days before the operation and continuing for 4 weeks, 0.2 mg/kg/d rosiglitazone was administered to the study group orally. Four weeks later rats were killed and ectopic uterine tissues were evaluated morphologically and histologically. Scoring systems were used to evaluate preservation of epithelia. Results: Four rats in the study group and one rat in the control group died of complications related to surgery. There was a significant difference in post-treatment spherical volumes (64.00 mm3 [interquartile range (IQR): 354.42] vs 41.60 mm3 [IQR: 37.87], P = .018) and explant weights (77.97 mg [IQR: 431.27] vs 47.24 mg [IQR: 43.01], P = .005) between control and rosiglitazone-treated groups. The epithelia were found to be preserved significantly better in the control group when compared with the roziglitazone-treated group (2.00 [IQR:2.00] vs 0.00 [IQR:2.25], P = .014). Conclusions: Rosiglitazone was found to affect the induction of endometriosis negatively in this experimental rat model and seemed to interfere with the growth and maintenance of the uterine explant.

Peroxisome proliferator‐activated receptor‐gamma agonist rosiglitazone reduces the size of experimental endometriosis in the rat model

Background:  The effect of rosiglitazone, an activator of peroxisome proliferator‐activated receptor‐gamma, on the growth of ectopic uterine tissue was assessed.

Comparison of the use of electrothermal bipolar vessel sealer with harmonic scalpel in total laparoscopic hysterectomy

Aim:  The aim of the present study was to compare the use of electrothermal bipolar vessel sealer (EBVS) with harmonic scalpel (HS) during total laparoscopic hysterectomy with respect to operation time, estimated blood loss and related complications.

A novel antibiotic, linezolid, reduces intraperitoneal adhesion formation in the rat uterine horn model

Objective. To examine the effects of linezolid in prevention of adhesion formation in a rat uterine horn model. Design. Prospective randomized study. Setting. University Experimental Animal Research Laboratory Center. Sample. Ninety female Wistar albino rats. Methods. A dose response study was first conducted with 60 Wistar albino rats that were randomly assigned to six equal groups by administering vehicle (control), 5 mg/kg (Group 1), 15 mg/kg (Group 2), 50 mg/kg (Group 3), 100 mg/kg (Group 4), and 150 mg/kg (Group 5) linezolid starting three days before the adhesion inducing operation and continuing for 14 days postoperatively. Adhesion was scored clinically with a scoring system. The minimum effective dose was found to be 100 mg/kg/day. With this dose time response (starting three days before the operation and continuing for seven days), only preoperative and postoperative administration studies were conducted. Main outcome measures. Extent and severity of adhesions. Results. Total adhesion scores in the control and Groups 1 and 2 were significantly higher when compared with Groups 4 and 5, but not with Group 3. There were no significant differences in the adhesion scores between Groups 3, 4, and 5. In time response arm a total of 10 days treatment was not as effective as 17 days treatment. In postoperative and preoperative arms of the study, it was found that administration of linezolid only postoperatively or preoperatively did not affect adhesion formation significantly when compared with the control group. Conclusion. Linezolid was found to reduce intraperitoneal adhesion formation.

Comparison of the adhesion scoring systems used in animal models and assessment of interobserver reproducibility

Two commonly used adhesion scoring systems were compared and the interobserver variability was assessed in 30 Wistar albino rats. Thirty‐five milligrams per kilogram leflunomide was administered orally and intraperitoneally. Two weeks later, adhesion formation was assessed using the two scoring systems. The results showed that the two currently used adhesion scoring systems are subjective and the final results change depending on the observer and the type of the scoring system that has been used.

Cervical Partial Hydatidiform Molar Pregnancy

Background: Cervical hydatidiform molar pregnancy is an exceedingly rare occurrence that has been reported only twice before. Case: We report a 25-year-old, G4P1D&C2 woman with a positive pregnancy test and vaginal bleeding. A cervical pregnancy with hydatidiform mole was detected on transvaginal ultrasound and color Doppler examinations. Magnetic resonance imaging was used to localize the lesion. Dilatation and curettage was performed and bleeding that was initially brisk ceased after bimanual pressure. Histopathological examination revealed a partial hydatidiform molar pregnancy. Serial β-hCG measurements showed a decline without need for adjuvant chemotherapy. Conclusion: Conservative fertility-sparing management was successful in this potentially fatal rare case.

Effects of ovarian drilling in middle Black Sea region Turkish women with polycystic ovary syndrome having normal and high body mass indices.

Aim:  To assess the effectiveness of laparoscopic ovarian drilling (LOD) in women with polycystic ovary syndrome (PCOS) with normal and high body mass indices (BMIs).

Altered expression of COP9 signalosome proteins in preeclampsia

Objective: The presumptive factors that are released by the preeclamptic placenta to cause maternal disease are less well known. The constitutive photomorphogenic-9 (COP9) signalosome (CSN) complex, a multifunctional protein complex involved in modulating signal transduction, gene transcription, and protein stability in cells. Although the roles of most CSN components in early embryonic development have been studied, their role in preeclamptic human placentas is not known. Thus, this study was aimed to show the localization and the protein expression of CSN1 and CSN5 in normal and preeclamptic placenta. Study design: The distribution and the protein expression of CSN1 and CSN5 were analyzed in normal (n: 15) and preeclamptic (n: 15) human placenta by using immunohistochemistry (IHC) and Western blotting. Results: CSN1 and CSN5 were mainly localized in the vascular endothelium, syncytiotrophoblast, stromal and Hofbauer cells in normal and preeclamptic placentas. However, a stronger immunoreactivity and protein expression for CSN1 and CSN5 were observed in preeclamptic placentas compared to normal term placentas. Western blotting of the tissue extracts confirmed the IHC results. Conclusions: Our results suggest that an increased level of CSN1 and CSN5 as an important part of the ubiquitin proteasome system (UPS) might be associated with the pathophysiology of preeclampsia.

A preliminary retrospective study about the relationship between ductus venosus Doppler indices, nuchal translucency (NT) and biochemical markers in the first and second trimester screening tests

In our study, we tried to assess the relation between ductus venosus Doppler indices [pulsatility index (PI), resistance index (RI) and S/D] and first-trimester screening markers (MoM of serum pregnancy-associated plasma protein A, pappalysin 1 (PAPP-A), MoM of serum free β-human chorionic gonadotrophin (β-hCG), and nuchal translucency (NT) and second trimester screening markers (MoM of serum α-fetoprotein, MoM of serum total β-hCG and MoM of serum estriol). We analyzed the data of 121 singleton pregnancies. Roche cobas e 601ECLIA (electrochemiluminescence immunoassay) was used to measure MoM of serum PAPP-A and Roche cobas e 602 ECLIA (electrochemiluminescence immunoassay) was used to measure MoM of serum free β-hCG in the first trimester. Beckman Coulter Access 2 Immunoassay was used to measure MoM of serum α-fetoprotein, MoM of serum total β-hCG and MoM of serum estriol in the second trimester. The first author performed all ultrasound screenings and ductus venosus Doppler studies. What we found new in our study is presented as following; MoM of serum α-fetoprotein had a negative correlation with RI of ductus venosus Doppler, MoM of serum estriol had a negative correlation with RI of ductus venosus Doppler and MoM of serum estriol had a negative correlation with S/D of ductus venosus doppler. The results of our study suggest that ductus venosus Doppler can be used to increase the effectiveness of the second trimester screening test.