Fazlı Demirtürk

Effect of Peroxisome Proliferator—Activated Receptor-γ Agonist Rosiglitazone on the Induction of Endometriosis in an Experimental Rat Model

Objective: To assess the effect of the peroxisome proliferator-activated receptor (PPAR)-γ agonist rosigliotazone on the induction of endometriosis in a rat model. Methods: Endometriosis was surgically induced in 28 rats by transplanting an autologous fragment of endometrial tissue onto the inner surface of the abdominal wall. Group I was assigned as control and no medication was administered. Starting 3 days before the operation and continuing for 4 weeks, 0.2 mg/kg/d rosiglitazone was administered to the study group orally. Four weeks later rats were killed and ectopic uterine tissues were evaluated morphologically and histologically. Scoring systems were used to evaluate preservation of epithelia. Results: Four rats in the study group and one rat in the control group died of complications related to surgery. There was a significant difference in post-treatment spherical volumes (64.00 mm3 [interquartile range (IQR): 354.42] vs 41.60 mm3 [IQR: 37.87], P = .018) and explant weights (77.97 mg [IQR: 431.27] vs 47.24 mg [IQR: 43.01], P = .005) between control and rosiglitazone-treated groups. The epithelia were found to be preserved significantly better in the control group when compared with the roziglitazone-treated group (2.00 [IQR:2.00] vs 0.00 [IQR:2.25], P = .014). Conclusions: Rosiglitazone was found to affect the induction of endometriosis negatively in this experimental rat model and seemed to interfere with the growth and maintenance of the uterine explant.

Peroxisome proliferator‐activated receptor‐gamma agonist rosiglitazone reduces the size of experimental endometriosis in the rat model

Background:  The effect of rosiglitazone, an activator of peroxisome proliferator‐activated receptor‐gamma, on the growth of ectopic uterine tissue was assessed.

Comparison of the use of electrothermal bipolar vessel sealer with harmonic scalpel in total laparoscopic hysterectomy

Aim:  The aim of the present study was to compare the use of electrothermal bipolar vessel sealer (EBVS) with harmonic scalpel (HS) during total laparoscopic hysterectomy with respect to operation time, estimated blood loss and related complications.

Evaluation of postural equilibrium and fall risk during pregnancy

OBJECTIVE The hormonal, anatomical and physiological states change during pregnancy. Due to these alterations, pregnant women are at increased risk of falling throughout this period. The aim of this study is to evaluate postural equilibrium and risk of falls during pregnancy by comparing dynamic postural stability between pregnant and non-pregnant control women. METHODS Eighty pregnant women (the first, second and third trimester of pregnancy; 25, 30 and 25, respectively) and thirty nonpregnant control women were evaluated and compared in terms of dynamic postural stability using the Biodex Balance System. Overall (OA), anterior-posterior (AP), medial-lateral (ML) stability index and fall risk test (FRT) scores were obtained from the mean scores of the three trials at platform stability of level 8. RESULTS No significant differences for OA, APSI and MLSI were found among the pregnant women in the first and second trimester and nonpregnant control subjects. Overall, anteroposterior and mediolateral index scores were significantly higher in pregnant women in the third trimester than nonpregnant controls (p<0.05). Fall risk test scores of third trimester patients were found to be significantly higher than the first and second trimester and nonpregnant control women (p<0.001). CONCLUSIONS Pregnancy has a negative effect on postural stability. Postural equilibrium decreases during pregnancy, particularly in the third trimester. Using postural stability tests may detect pregnant women with a high fall risk.

A novel antibiotic, linezolid, reduces intraperitoneal adhesion formation in the rat uterine horn model

Objective. To examine the effects of linezolid in prevention of adhesion formation in a rat uterine horn model. Design. Prospective randomized study. Setting. University Experimental Animal Research Laboratory Center. Sample. Ninety female Wistar albino rats. Methods. A dose response study was first conducted with 60 Wistar albino rats that were randomly assigned to six equal groups by administering vehicle (control), 5 mg/kg (Group 1), 15 mg/kg (Group 2), 50 mg/kg (Group 3), 100 mg/kg (Group 4), and 150 mg/kg (Group 5) linezolid starting three days before the adhesion inducing operation and continuing for 14 days postoperatively. Adhesion was scored clinically with a scoring system. The minimum effective dose was found to be 100 mg/kg/day. With this dose time response (starting three days before the operation and continuing for seven days), only preoperative and postoperative administration studies were conducted. Main outcome measures. Extent and severity of adhesions. Results. Total adhesion scores in the control and Groups 1 and 2 were significantly higher when compared with Groups 4 and 5, but not with Group 3. There were no significant differences in the adhesion scores between Groups 3, 4, and 5. In time response arm a total of 10 days treatment was not as effective as 17 days treatment. In postoperative and preoperative arms of the study, it was found that administration of linezolid only postoperatively or preoperatively did not affect adhesion formation significantly when compared with the control group. Conclusion. Linezolid was found to reduce intraperitoneal adhesion formation.

Prognostic Importance of the Nucleolar Organizer Region Score in Ovarian Epithelial Tumors

Objective: The score of the silver-stained nucleolar organizer region (AgNOR) is frequently found to be high in malignant tumors. We studied AgNOR in ovarian epithelial tumors diagnosed in our hospital between 1993 and 1998. Materials and Method: In our study 67 ovarian epithelial tumors (18 cystadenoma, 16 borderline type, 34 cystadenocarcinoma) were stained using the method previously described by Crocker. One-way ANOVA and logistic regression tests were used to find the statistical relationship between grade, recurrence, mortality rates and AgNOR scores in tumors (p values of <0.05 were considered statistically significant). Results: The mean AgNOR scores of 28 mucinous and 39 serous ovary tumors were calculated. The lowest AgNOR score of 1.33 was found in cystadenomas and the highest AgNOR score of 4.92 was found in serous and mucinous cystadenocarcinomas. In addition the relationship between mortality rate, recurrence and AgNOR score in carcinomas were studied. Conclusion: AgNOR scores in carcinomas were found to be higher than adenomas, and the highest AgNOR score was found in grade-III carcinomas. This shows that the AgNOR score can be used as a prognostic index in malignancies.

Comparison of the adhesion scoring systems used in animal models and assessment of interobserver reproducibility

Two commonly used adhesion scoring systems were compared and the interobserver variability was assessed in 30 Wistar albino rats. Thirty‐five milligrams per kilogram leflunomide was administered orally and intraperitoneally. Two weeks later, adhesion formation was assessed using the two scoring systems. The results showed that the two currently used adhesion scoring systems are subjective and the final results change depending on the observer and the type of the scoring system that has been used.

Cervical Partial Hydatidiform Molar Pregnancy

Background: Cervical hydatidiform molar pregnancy is an exceedingly rare occurrence that has been reported only twice before. Case: We report a 25-year-old, G4P1D&C2 woman with a positive pregnancy test and vaginal bleeding. A cervical pregnancy with hydatidiform mole was detected on transvaginal ultrasound and color Doppler examinations. Magnetic resonance imaging was used to localize the lesion. Dilatation and curettage was performed and bleeding that was initially brisk ceased after bimanual pressure. Histopathological examination revealed a partial hydatidiform molar pregnancy. Serial β-hCG measurements showed a decline without need for adjuvant chemotherapy. Conclusion: Conservative fertility-sparing management was successful in this potentially fatal rare case.

Role of first trimester total testosterone in prediction of subsequent gestational diabetes mellitus

To assess the role of first trimester maternal testosterone and dehydroepiandrosterone sulfate (DHEA‐S) levels in prediction of development of gestational diabetes mellitus (GDM).

Effects of ovarian drilling in middle Black Sea region Turkish women with polycystic ovary syndrome having normal and high body mass indices.

Aim:  To assess the effectiveness of laparoscopic ovarian drilling (LOD) in women with polycystic ovary syndrome (PCOS) with normal and high body mass indices (BMIs).