Akimitsu Kugimiya

Surface plasmon resonance sensor using molecularly imprinted polymer for detection of sialic acid

A surface plasmon resonance (SPR) sensor using a molecularly imprinted polymer-coated sensor chip for the detection of sialic acid was developed. The thinly coated polymer was prepared by co-polymerizing N,N,N-trimethylaminoethyl methacrylate, 2-hydroxyethyl methacrylate and ethyleneglycol dimethacrylate in the presence of p-vinylbenzeneboronic acid ester with sialic acid. The sensor showed a selective response to ganglioside of which sialic acid is located at the non-reducing end and gave a linear relationship from 0.1 to 1.0 mg of ganglioside.

A Biomimetic Receptor System for Sialic Acid Based on Molecular Imprinting

Abstract A new type of a fluorescent receptor system specific for sialic acid has been prepared. For the preparation of this artificial receptor a polymerization reaction using allylamine, vinylphenylboronic acid and ethylene glycol dimethacrylate in the presence of sialic acid as template was carried out. After splitting off the template molecules, a suspension of the polymer was used in reaction with OPA reagents, both in the presence and absence of sugars. It was found, that sialic acid promoted the fluorescence development in this system, probably as a result of the so-called “gate effect”, resembling natural occurring receptors. Rapid detection of sialic acid in the concentration range of 0.5–10 μM was shown possible within 40 minutes. Our method seems applicable for the development of optical sensors; combining the high sensitivity and selectivity of biological receptors and high stability of synthetic polymeric materials.

Molecularly Imprinted Polymer-Coated Quartz Crystal Microbalance for Detection of Biological Hormone

A piezoelectric sensor with a molecularly imprinted synthetic polymer receptor was developed. A plant hormone, indole-3-acetic acid (IAA) was used as the model target molecule, and an IAA-imprinted polymer was coated onto a 9 MHz AT-cut quartz crystal microbalance. The sensor showed a selective response and gave a linear relationship between frequency shift and amount of IAA in the range from 10 to 200 nmol.

Recognition of sialic acid using molecularly imprinted polymer

Abstract The molecular imprinting technique was applied for the preparation of a polymer selective for sialic acid. To evaluate its binding ability the molecularly imprinted polymer obtained was used as a stationary phase in liquid chromatography. The polymer showed pH-dependent characteristics for binding: an optimum specificity to sialic acid at pH 8.1 and a higher affinity with group selectivity for cis-diol containing sugars at higher pH.

Preparation of sterol-imprinted polymers with the use of 2-(methacryloyloxy)ethyl phosphate

Steroid-selective polymers were prepared by the molecular imprinting technique, using 2-(methacryloyloxy)ethyl phosphate as functional monomer. The retentivity and selectivity of the obtained imprinted polymers were evaluated by liquid chromatography. The cholesterol-imprinted polymer showed higher affinity for cholesterol than that for cholesterol derivatives. The selectivity of the imprinted polymer was superior to the imprinted polymer prepared with the conventional functional monomer, 2-(trifluoromethyl)acrylic acid. Estradiol was also imprinted and gave similar results, demonstrating that 2-(methacryloyloxy)ethyl phosphate would be suitable for imprinted polymers of cholesterol and related compounds.

Recognition in Novel Molecularly Imprinted Polymer Sialic Acid Receptors in Aqueous Media

Abstract A molecularly imprinted polymer selective for sialic acid was prepared using two functional monomers, p-vinylbenzeneboronic acid and N, N, N-trimethylaminoethyl methacrylate chloride in 2-hydroxyethyl methacrylate/ethyleneglycol dimethacrylate based copolymers, and specific binding of the target compound sialic acid was achieved in aqueous solution. Present address: Laboratory of Synthetic Biochemistry, Faculty of Information Sciences, Hiroshima City University

Synthesis of castasterone selective polymers prepared by molecular imprinting

Castasterone-selective polymers were prepared by molecular imprinting using p-vinylbenzeneboronic acid or methacrylic acid as a functional monomer. Each molecularly imprinted polymer showed selectivity for castasterone in liquid chromatography.

Synthesis of 5-fluorouracil-imprinted polymers with multiple hydrogen bonding interactions.

The antitumor active compound 5-fluorouracil (5-FU) was used as a target molecule and 5-FU-imprinted polymers were synthesized using 2,6-bis(acrylamido)pyridine and/or 2-(trifluoromethyl)acrylic acid as functional monomers. The 5-FU-imprinted polymers showed a higher affinity for 5-FU than that for 5-FU derivatives. By using both functional monomers simultaneously, the affinity and separation for 5-FU were improved.

Application of indoleacetic acid-imprinted polymer to solid phase extraction

Indole-3-acetic acid-selective polymers were prepared by molecular imprinting, and the imprinted polymer was applied to the solid phase extraction. At first, the effect of functional monomers, N,N-dimethylaminoethyl methacrylate and methacrylic acid, on affinities in imprinted polymers prepared was examined chromatographically. The imprinted polymer prepared with N,N-dimethylaminoethyl methacrylate showed higher affinity for indole-3-acetic acid than the polymer prepared with methacrylic acid, and the imprinted polymer was applied to the solid phase extraction.

Sialic Acid Imprinted Polymer-Coated Quartz Crystal Microbalance

Molecularly imprinted polymer-coated sensors for sialic acid were developed using a 9 MHz AT-cut quartz crystal microbalance (QCM). Recognition chips of the sensors used in this study were prepared by coating the two types of sialic acid-imprinted polymers with the use of p-vinylbenzeneboronic acid and/or N,N,N-trimethylaminoethyl methacrylate onto QCM. Each piezoelectric sensor showed a selective response to sialic acid and gave a linear relationship between frequency shift and amount of sialic acid from 20 to 250 nmol.