Alan L. Wright

OIL BIOREMEDIATION IN SALT MARSH MESOCOSMS AS INFLUENCED BY N AND P FERTILIZATION, FLOODING, AND SEASON

Bioremediation of crude oil in salt marsh mesocosms growingSpartina alterniflora was investigated during winter and summer to determine the influence of nitrogen (N) and phosphorus (P) fertilization, flooding, and season. Fertilization with urea and ammonium (NH4+) applied at 75 or 150 kg N ha−1 with or without P did not significantly (p=0.05) increase oil or hydrocarbon degradation in continuously flooded mesocosms over an 82 day period during winter (temperature range of 17 to 30 °C). Phosphorus applied at 40 kg P ha−1 significantly (p = 0.05) increased oil and hydrocarbon degradation. Nitrate (NO3−) ) added alone did not increase oil or hydrocarbon degradation, but when added with P, it significantly (p = 0.05) increased degradation above that for P alone. Up to 70% of applied oil and 75% of applied hydrocarbons were degraded in P supplemented treatments. Inipol, an oleophilic fertilizer containing N, P, and a dispersant, significantly increased oil and hydrocarbon degradation. During a 40 day summer experiment (temperature range of 27–42 °C), N and P fertilization did not increase oil or hydrocarbon degradation. For continuously flooded treatments, 72% of applied hydrocarbons were degraded while 51% were degraded in alternately flooded treatments. Mesocosms provided conditions suitable for quantitative recovery of oil and results indicated that N and P fertilization, flooding, and season interacted to influence oil bioremediation. Even under the most favorable conditions, more than 1 month was required for most of the oil to disappear.

Loss‐on‐Ignition Method to Assess Soil Organic Carbon in Calcareous Everglades Wetlands

Measurement of soil carbon (C) is important for determining the effects of Everglades restoration projects on C cycling and transformations. Accurate measurement of soil organic C by automated carbon–nitrogen–sulfur (CNS) analysis may be confounded by the presence of calcium carbonate (CaCO3) in Everglades wetlands. The objectives of this study were to compare a loss‐on‐ignition (LOI) method with CNS analysis for assessment of soil C across a diverse group of calcareous Everglades wetlands. More than 3168 samples were taken from three soil depths (floc, 0–10, 10–30 cm) in 14 wetlands and analyzed for LOI, total C, and total calcium (Ca). The LOI method compared favorably to CNS analysis for LOI contents ranging from 0 to 1000 g kg−1 and for soil total Ca levels from 0 to 500 g Ca kg−1. For all wetlands and soil depths, LOI was significantly related to total C (r2 = 0.957). However, LOI was a better predictor of total C when LOI exceeded 400 g kg−1 because of less interference by CaCO3. Total C measurement by CNS analysis was problematic in soils with high total Ca and low LOI, as the presence of CaCO3 confounded C analysis for LOI less than 400 g kg−1. Inclusion of total Ca in regression models with LOI significantly improved the prediction of total C. Estimates of total organic C by CNS analysis were obtained by accounting for C associated with CaCO3 by calculation, with results being similar to total organic C values obtained from LOI analysis. The proportion of C in organic matter measured by the LOI method (51%) was accurate and applicable across wetlands, soil depths, and total Ca levels; thus LOI was a suitable indicator of total organic C in Everglades wetlands.

Crop Species And Tillage Effects On Carbon Sequestration In Subsurface Soil

Crop species and conservation tillage may enhance carbon (C) and nitrogen (N) sequestration potential in subsurface soils. The objectives of this study were to determine the effects of crop species and tillage on soil organic C (SOC) and total N distribution in six soil depth intervals from 0 to 105 cm after 20 years of treatment imposition. Tillage had the most influence on soil C and N at 0 to 5 cm, and impacts extended to the 15- to 30-cm depth for wheat and sorghum. Overall, SOC and total N for wheat were 18 and 15% higher than sorghum and soybean. Dissolved organic C (DOC) depth distribution was similar to SOC and total N. The proportion of SOC as DOC ranged from 1.3 to 3.3% and increased with soil depth. The highest soil C and N levels occurred for wheat under no tillage. The depth of soil impacted by crop species was shallower for conventional tillage than no tillage, and the depth distribution exhibited a logarithmic pattern. Soil organic C, total N, and DOC decreased 404, 507, and 205%, respectively from 0-5 to 80-105 cm. The maximum depth interval below which no further decreases in SOC and total N occurred was 30 to 55 cm for soybean, 55 to 80 cm for wheat, and 80 to 105 cm for sorghum, demonstrating the importance of subsurface soils for C sequestration. Crop management impacts below the depth of tillage demonstrate the importance of crop rooting and belowground biomass, or translocation of dissolved organic matter, to subsoil C sequestration.

Land‐Use Effects on Soil Nutrient Cycling and Microbial Community Dynamics in the Everglades Agricultural Area, Florida

Soil subsidence has become a critical problem since the onset of drainage of the organic soils in the Everglades Agricultural Area (EAA), which may impair current land uses in the future. The objectives of this study were to characterize soil microbial community‐level physiology profiles, extracellular enzymatic activities, microbial biomass, and nutrient pools for four land uses: sugarcane, turfgrass, pasture, and forest. Long‐term cultivation and management significantly altered the distribution and cycling of nutrients and microbial community composition and activity in the EAA, especially for sugarcane and turf fields. The least‐managed fields under pasture had the lowest microbial biomass and phosphorus (P) levels. Turf and forest had more microbial metabolic diversity than pasture or the most intensively managed sugarcane fields. Land‐use changes from sugarcane cropping to turf increased microbial activity and organic‐matter decomposition rates, indicating that changes from agricultural to urban land uses may further contribute to soil subsidence.

Fertilization and Bioaugmentation for Oil Biodegradation in Salt Marsh Mesocosms

Biodegradation of crude oil is often dependent on the population sizes and metabolic activity of hydrocarbon-degrading microorganisms in addition to nutrient supply. Fertilization with N and P and bioaugmentation of oil-contaminated soil with hydrocarbon-degrading microorganisms may serve to enhance oil biodegradation rates. Glasshouse experiments were conducted to determine the impacts of fertilization and commercial bioremediation products on crude oil biodegradation and on changes in nutrient concentrations and populations of hydrocarbon-degrading microorganisms in salt marsh mesocosms growing Spartina alterniflora. Experiments were conducted under continuously-flooded and alternately-flooded/drained conditions with and without N and P fertilization. MaxBac, a slow-release fertilizer, was applied at a rate of 100 kg N ha-1 and 20 kg P ha-1, while additional P was applied at 20 kg P ha-1. Commercial products failed to enhance total oil or total petroleum hydrocarbon (TPH) degradation under either continuously or alternately-flooded conditions. An average of 62% of TPH was degraded by 33 d under continuously-flooded conditions, while 59% was degraded by 41 d after oil application under alternately-flooded conditions. Products generally did not increase population sizes of heterotrophs orhydrocarbon-degrading microorganisms. Concentrations of NH4+ and P decreased during experimentation, and fertilization with N and P stimulated total oil and TPH degradation under continuously-flooded, but not under alternately-flooded conditions.

Dissolved and Soil Organic Carbon after Long‐Term Conventional and No‐Tillage Sorghum Cropping

Abstract Distribution of dissolved (DOC) and soil organic carbon (SOC) with depth may indicate soil and crop‐management effects on subsurface soil C sequestration. The objectives of this study were to investigate impacts of conventional tillage (CT), no tillage (NT), and cropping sequence on the depth distribution of DOC, SOC, and total nitrogen (N) for a silty clay loam soil after 20 years of continuous sorghum cropping. Conventional tillage consisted of disking, chiseling, ridging, and residue incorporation into soil, while residues remained on the soil surface for NT. Soil was sampled from six depth intervals ranging from 0 to 105 cm. Tillage effects on DOC and total N were primarily observed at 0–5 cm, whereas cropping sequence effects were observed to 55 cm. Soil organic carbon (C) was higher under NT than CT at 0–5 cm but higher under CT for subsurface soils. Dissolved organic C, SOC, and total N were 37, 36, and 66%, respectively, greater under NT than CT at 0–5 cm, and 171, 659, and 837% greater at 0–5 than 80–105 cm. The DOC decreased with each depth increment and averaged 18% higher under a sorghum–wheat–soybean rotation than a continuous sorghum monoculture. Both SOC and total N were higher for sorghum–wheat–soybean than continuous sorghum from 0–55 cm. Conventional tillage increased SOC and DOC in subsurface soils for intensive crop rotations, indicating that assessment of C in subsurface soils may be important for determining effects of tillage practices and crop rotations on soil C sequestration.

Sulfur Distribution and Transformations in Everglades Agricultural Area Soil as Influenced by Sulfur Amendment

Nutrient export from the Everglades Agricultural Area (EAA) has been implicated in causing sulfur (S) enrichment of Everglades wetlands. However, quantification of the S budget and transformations in EAA soils is inadequate. The objective of this study was to quantify various S fractions and investigate how elemental S amendment affects S dynamics in EAA soils. Reduced S compounds were not detected in soil before elemental S application. Organic S was the major form of S, comprising 87% of total S, followed by extractable SO4-S (13%). Extractable SO4-S for soils receiving 448 kg S ha−1 was 36%, 131%, 201%, and 270% higher than for unamended soils at 2, 6, 9, and 13 months, respectively. Elemental S was significantly higher in soils receiving 448 kg S ha−1 (482 mg kg−1) than in soils receiving 224 (111 mg kg−1) and 112 kg S ha-1 (55 mg kg−1) and unamended soil (0 mg kg−1) at 2 months after S application. Similar to extractable SO4-S, elemental S significantly decreased during the growing season. Sulfur application did not affect the sulfatase activity, however, mineralizable S increased concurrently with S application rate, and the effects continued throughout the growing season. This result was largely attributed to the oxidation of the applied elemental S. Our results suggest that large-scale S application in the EAA soils is likely to increase SO42− concentrations in soils, which poses a potential risk of SO42− export to sensitive Everglades wetlands.

Compost Source and Rate Effects on Soil Macronutrient Availability Under Saint Augustine Grass and Bermuda Grass Turf

Compost application to turf grasses can increase availability of nutrients in soil and improve growth, but can potentially lead to accumulation of macronutrients in soil and contribute to leaching and runoff losses. The objectives of this study were to investigate the influence of compost source and application rate on concentrations of plant-available macronutrients in soil over 29 months after a one-time application to saint augustine grass [Stenotaphrum secundatum (Walt.) Kuntze] and Bermuda grass [Cynodon dactylon (L.) Pers.] turf. Compost application increased soil organic C, P, Ca, and S concentrations by 3 months after addition, but further increases from 3 to 29 months were seldom observed. In contrast, NO3-N and K levels declined while Mg levels increased slightly from 3 to 29 months. Seasonal or cyclical patterns of soil macronutrient levels were apparent, as lower concentrations were observed during dormant stages of Bermuda grass growth in winter. Initial macronutrient concentrations of compost sources strongly influenced macronutrient dynamics in surface soil, while higher application rates resulted in higher levels of P, K, Ca, Mg, but not NO3-N and S. Higher levels of macronutrients in Bermuda grass than saint augustine grass turf suggested plant-mediated uptake and assimilation differed between turf grass species. Utilization of turf grass systems for compost application should take into account plant species composition and the related impacts of plant uptake. Macronutrient concentrations were significantly correlated with both total organic C and dissolved organic C (DOC). Formation of organic matter-cation complexes appeared to influence macronutrient dynamics in soil, and may contribute to leaching and runoff losses.

SEASONAL CHANGES IN NUTRIENT AVAILABILITY FOR SULFUR-AMENDED EVERGLADES SOILS UNDER SUGARCANE

The objective of this study was to evaluate effects of elemental sulfur (S) addition on soil pH and availability of macro- and micronutrients during the sugarcane growing season. Sulfur application did not significantly reduce soil pH when applied at 0 to 448 kg S ha−1 due to the high soil buffering capacity. Water extractable phosphorus (P) and potassium (K) for soils receiving the highest S rate were 188% and 71% higher than for unamended soils only at two months after application, indicating a short-term enhancement of macronutrient availability. Soil amended with 448 kg S ha−1 contained 134% more acetic acid-extractable zinc (Zn) than unamended soil, although stimulatory effects did not extend beyond two months. Sugar yield was not affected by S addition, averaging 17 Mg sugar ha−1. The failure of S to enhance nutrient availability throughout the growing season indicates the limited benefit of applying elemental S to reduce pH and increase nutrient availability to sugarcane.

Compost Impacts on Sodicity and Salinity In a Sandy Loam Turf Grass Soil

Compost application to turf grass may influence soil sodicity and salinity and eventually the establishment and growth of turf grass. The objectives of this study were to determine effects of compost source and application rate on soil sodicity and salinity during 29 months after a one-time application to Saint Augustine grass and Bermuda grass turf grown on a sandy loam soil. Extractable soil Na, electrical conductivity (EC), and pH did not differ among compost sources having variable Na and nutrient levels. However, compost application decreased soil Na, EC, and pH compared to unamended soil likely due to high applications of Ca, Mg, and K, which occupied cation-exchange sites on soil particles, minimizing adsorption of Na and enhancing Na leaching losses during precipitation events. Furthermore, high application of composts increased soil dissolved organic C (DOC) levels, which may have coated soil particles and limited adsorption of Na. Complexation of extractable cations with DOC, followed by potential leaching of DOC-associated cations, tended to decrease soil EC. Thus, composts may actually serve to alleviate soil sodicity and salinity problems. Seasonal variation of extractable soil Na and EC were related to growth stages of turf grass, which influenced DOC levels, and precipitation patterns, which influenced vertical movement of DOC-associated cations.