Tony L. Provin

Not just the usual suspects: insect herbivore populations and communities are associated with multiple plant nutrients

The relationship between plant nutrient content and insect herbivore populations and community structure has long interested ecologists. Insect herbivores require multiple nutrients, but ecologists have focused mostly on nitrogen (an estimate of plant protein content), and more recently phosphorus (P); other nutrients have received little attention. Here we document nutrient variation in grass and forb samples from grassland habitats in central Nebraska using an elemental approach; in total we measured foliar concentrations of 12 elements (N and P, plus S, B, Ca, Mg, Na, K, Zn, Fe, Mn, and Cu). We detected significant variability among sites for N, P, Mg, Na, K, and Cu. We next used a model selection approach to explore how this nutritional variation and plant biomass correlate with grasshopper densities (collectively and at the feeding-guild level), and principal component analysis to explore nutrient correlations with grasshopper community species composition. When all grasshoppers were pooled, densities varied among sites, but only P was associated with abundance of the elements shown to vary between sites. Different responses occurred at the feeding-guild level. For grass specialists, densities were associated with N, plus P, Mg, and Na. For forb specialists, N and P were often associated with density, but associations with Na and K were also observed. Finally, mixed-feeder abundance was strongly associated with biomass, and to a lesser extent P, Mg, Na, and Cu. At the community level, B, Ca, Zn, and Cu, plus biomass, explained > 30% of species composition variation. Our results confirm the positive association of N and P with insect herbivore populations, while suggesting a potential role for Mg, Na, and K. They also demonstrate the importance of exploring effects at the feeding-guild level. We hope our data motivate ecologists to think beyond N and P when considering plant nutrient effects on insect herbivores, and make a call for studies to examine functional responses of insect herbivores to dietary manipulation of Mg, Na, and K. Finally, our results demonstrate correlations between variation in nutrients and species assemblages, but factors not linked to plant nutrient quality or biomass likely explain most of the observed variation.

SIMULATING WATER QUALITY IMPROVEMENTS IN THE UPPER NORTH BOSQUE RIVER WATERSHED DUE TO PHOSPHORUS EXPORT THROUGH TURFGRASS SOD

The Upper North Bosque River (UNBR) watershed is under a total maximum daily load (TMDL) mandate to reduce loading of soluble phosphorus (P) in impaired river segments. To address these problems, Texas A&M University researchers have developed a Best Management Practice (BMP) that removes excess nutrients from impaired watersheds through turfgrass sod. Harvest of manure-grown sod removes a thin layer of topsoil along with any residual P in this soil layer. In order to assess the impact of the turfgrass BMP on a watershed scale, the Soil and Water Assessment Tool (SWAT) was used to predict water quality changes among four scenarios in the UNBR watershed. The SWAT model was modified to incorporate turfgrass harvest routines for simulation of manure and soil P export during harvest of turfgrass sod. SWAT simulations of the four BMP scenarios predicted reductions of 20% to 36% for instream P loads in the UNBR depending on manure P rate and areas allotted to sod. In addition, total N load was reduced on average by 31% and sediment load declined on average 16.7% at the watershed outlet. The SWAT model predicted up to 176 kg/ha P was removed per harvest of sod top-dressed with 100 kg manure P/ha. Export increased to 258 kg/ha of P per harvest for the manure P application rate of 200 kg/ha. Depending on the implementation scenario, simulations indicated the turfgrass BMP could export between 262 and 784 metric tons of P out of the UNBR watershed every year.

Capacity of biochar application to maintain energy crop productivity: soil chemistry, sorghum growth, and runoff water quality effects.

Pyrolysis of crop biomass generates a by-product, biochar, which can be recycled to sustain nutrient and organic C concentrations in biomass production fields. We evaluated effects of biochar rate and application method on soil properties, nutrient balance, biomass production, and water quality. Three replications of eight sorghum [ (L.) Moench] treatments were installed in box lysimeters under greenhouse conditions. Treatments comprised increasing rates (0, 1.5, and 3.0 Mg ha) of topdressed or incorporated biochar supplemented with N fertilizer or N, P, and K fertilizer. Simulated rain was applied at 21 and 34 d after planting, and mass runoff loss of N, P, and K was measured. A mass balance of total N, P, and K was performed after 45 d. Returning 3.0 Mg ha of biochar did not affect sorghum biomass, soil total, or Mehlich-3-extractable nutrients compared to control soil. Yet, biochar contributed to increased concentration of dissolved reactive phosphorus (DRP) and mass loss of total phosphorus (TP) in simulated runoff, especially if topdressed. It was estimated that up to 20% of TP in topdressed biochar was lost in surface runoff after two rain events. Poor recovery of nutrients during pyrolysis and excessive runoff loss of nutrients for topdressed biochar, especially K, resulted in negative nutrient balances. Efforts to conserve nutrients during pyrolysis and incorporation of biochar at rates derived from annual biomass yields will be necessary for biochar use in sustainable energy crop production.

Impact of Indian Mustard (Brassica juncea) and Flax (Linum usitatissimum) Seed Meal Applications on Soil Carbon, Nitrogen, and Microbial Dynamics

There is a critical need to investigate how land application of dedicated biofuel oilseed meals affects soil ecosystems. In this study, mustard (Brassica juncea) and flax (Linum usitatissimum) seed meals and sorghum-sudangrass (Sorghum bicolor) were added to soil at levels of 0, 1, 2.5, and 5% (w/w). Both the type of amendment and application rate affected soil organic C, total C & N, and C & N mineralization. Mustard meal amendment initially inhibited C mineralization as compared to flax, but >50% of mustard and flax organic C was mineralized within 51 d. Nitrogen mineralization was similar for flax and mustard, except for the 2.5% rate for which a lower proportion of mustard N was converted to nitrate. The mustard meal greatly impacted microbial community composition, appearing to select for specific fungal populations. The potential varying impacts of different oilseed meals on soil ecosystems should be considered when developing recommendations for land application.

The relationship between body mass and elemental composition in nymphs of the grasshopper Schistocerca americana

Abstract Grasshoppers, like all other organisms, are composed of a combination of elements, but little is known about the extent to which the elemental composition in grasshoppers changes over the course of nymphal development. In this study the grasshopper Schistocerca americana was reared on a diet of seedling wheat and wheat germ; nymphs of various mass were collected and analyzed for elemental composition. In total, 12 different elements, including the macroelements carbon, nitrogen, and phosphorus, were quantified. Results show that the amount of a given element increased linearly with increasing body mass, but that the rate of increase differed depending on the element. Results also show that the concentration of some elements dropped dramatically over the course of development. We discuss our results in the context of limiting nutrients and ecological stoichiometry.

Compost Source and Rate Effects on Soil Macronutrient Availability Under Saint Augustine Grass and Bermuda Grass Turf

Compost application to turf grasses can increase availability of nutrients in soil and improve growth, but can potentially lead to accumulation of macronutrients in soil and contribute to leaching and runoff losses. The objectives of this study were to investigate the influence of compost source and application rate on concentrations of plant-available macronutrients in soil over 29 months after a one-time application to saint augustine grass [Stenotaphrum secundatum (Walt.) Kuntze] and Bermuda grass [Cynodon dactylon (L.) Pers.] turf. Compost application increased soil organic C, P, Ca, and S concentrations by 3 months after addition, but further increases from 3 to 29 months were seldom observed. In contrast, NO3-N and K levels declined while Mg levels increased slightly from 3 to 29 months. Seasonal or cyclical patterns of soil macronutrient levels were apparent, as lower concentrations were observed during dormant stages of Bermuda grass growth in winter. Initial macronutrient concentrations of compost sources strongly influenced macronutrient dynamics in surface soil, while higher application rates resulted in higher levels of P, K, Ca, Mg, but not NO3-N and S. Higher levels of macronutrients in Bermuda grass than saint augustine grass turf suggested plant-mediated uptake and assimilation differed between turf grass species. Utilization of turf grass systems for compost application should take into account plant species composition and the related impacts of plant uptake. Macronutrient concentrations were significantly correlated with both total organic C and dissolved organic C (DOC). Formation of organic matter-cation complexes appeared to influence macronutrient dynamics in soil, and may contribute to leaching and runoff losses.

Compost Impacts on Sodicity and Salinity In a Sandy Loam Turf Grass Soil

Compost application to turf grass may influence soil sodicity and salinity and eventually the establishment and growth of turf grass. The objectives of this study were to determine effects of compost source and application rate on soil sodicity and salinity during 29 months after a one-time application to Saint Augustine grass and Bermuda grass turf grown on a sandy loam soil. Extractable soil Na, electrical conductivity (EC), and pH did not differ among compost sources having variable Na and nutrient levels. However, compost application decreased soil Na, EC, and pH compared to unamended soil likely due to high applications of Ca, Mg, and K, which occupied cation-exchange sites on soil particles, minimizing adsorption of Na and enhancing Na leaching losses during precipitation events. Furthermore, high application of composts increased soil dissolved organic C (DOC) levels, which may have coated soil particles and limited adsorption of Na. Complexation of extractable cations with DOC, followed by potential leaching of DOC-associated cations, tended to decrease soil EC. Thus, composts may actually serve to alleviate soil sodicity and salinity problems. Seasonal variation of extractable soil Na and EC were related to growth stages of turf grass, which influenced DOC levels, and precipitation patterns, which influenced vertical movement of DOC-associated cations.

Effect of Turfgrass Establishment Practices and Composted Biosolids on Water Quality

Land application of composted municipal biosolids (CMB) enhances soil physical properties and turf establishment. Yet large, volume-based rates of CMB can increase nonpoint source losses of sediment and nutrients from urban soils to surface waters. The objectives were (i) to compare runoff losses of sediment, N, P, and organic C among contrasting establishment treatments for bermudagrass [Cynodon dactylon (L.) Pers. x C. transvaalensis Burtt-Davy, var. Tifway] and (ii) to evaluate relationships between runoff and soil measurements of N, P, and organic C. Three replications of seven establishment treatments were installed on an excavated slope (8.5%) under field conditions. Five treatments comprised sod transplanted from Tifway bermudagrass grown with and without CMB on soil with and without incorporation of CMB. Two other treatments were composed of Tifway sprigged in soil with and without CMB. Runoff from seven natural rain events was channeled into collection tanks for sampling and analysis. Runoff concentrations and mass loss of dissolved P and organic C forms were greater for CMB-amended sod than for sprigs planted in soil with or without CMB or treatments comprising sod established without CMB. In addition, a linear relationship (R(2) = 0.87) was observed between water extractable soil P of sodded and sprigged treatments and concentrations and mass losses of dissolved P in runoff. Transplanted sod reduced sediment loss compared with sprigged treatments and incorporation of CMB reduced sediment loss from sprigged treatments. Incorporation of CMB within soil on which sod grown without CMB was transplanted proved the best option for achieving benefits of CMB while reducing nutrient runoff loss compared to sod transplanted from Tifway grown with CMB.

Phosphorus Fertilization of Annual Ryegrass and Comparison of Soil Phosphorus Extractants

ABSTRACT It is unclear how much phosphorus (P) fertilizer is needed to maximize annual ryegrass (Lolium multiflorum Lam.cv. ‘TAM90’) forage yield and nutritive value in lower-rainfall climates. It is equally unclear which soil-P extractant is most effective when making P fertilization decisions. A 3-yr field study addressed these issues using small replicated plots during the winter months in north-central Texas. The addition of P increased yields every year of the trial, but optimum levels depended on rainfall distribution: 19 kg P ha− 1 yr− 1 was optimum two years, while 28 kg P ha− 1 yr− 1 increased yields 51% the year with good mid-season moisture. The addition of P fertilizer did not increase forage crude protein or affect fiber concentrations but did increase P yields. Percent relative yield of ryegrass was correlated with Mehlich III soil test P, soil pH, and P application rate. Conversely, the acidified NH4OAc-EDTA soil P extractant failed to relate to percent relative yield.

Biosolid and Alum effects on runoff losses during turfgrass establishment

Large, volume-based rates of composted biosolids (CB) enhance turfgrass establishment and soil properties, but nonpoint-source runoff losses could occur during production and after transplanting of sod. The objective was to evaluate runoff losses of N, P, sediment, and organic C during establishment of sprigs or transplanted sod of Tifway bermudagrass (Cynodon dactylon L. Pers. X C. transvaalensis Burtt-Davey) with and without CB and aluminum sulfate (Alum). Four treatments comprised Tifway sprigged in a sandy loam soil with and without incorporation of 0.25 m(3) CB m(-3) soil and Alum. In four additional treatments, sod transplanted from Tifway grown with and without CB was established with and without a surface spray of Alum. During early establishment, CB incorporated in soil before sprigging reduced runoff loss of sediment and total N to amounts comparable to transplanted sod. In contrast, mean runoff losses of total dissolved P and soluble-reactive P (SRP) were more than 50% greater for CB-amended sod than for fertilizer-grown sod or Tifway sprigged in soil with or without CB. Yet, the surface spray of Alum reduced runoff loss from sod more than 88% for SRP and 41% for dissolved organic C. Both surface sprays and incorporation of Alum effectively reduced SRP runoff loss from CB, soil, and turfgrass sources during turfgrass establishment.